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- Harreither, E., et al.
(författare)
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Characterization of a novel cell penetrating peptide derived from human Oct4
- 2014
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Ingår i: Cell Regeneration. - : Springer Science and Business Media LLC. - 2045-9769. ; 3:2, s. 2-3:2
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Tidskriftsartikel (refereegranskat)abstract
- Oct4 is a transcription factor that plays a major role for the preservation of the pluripotent state in embryonic stem cells as well as for efficient reprogramming of somatic cells to induced pluripotent stem cells (iPSC) or other progenitors. Protein-based reprogramming methods mainly rely on the addition of a fused cell penetrating peptide. This study describes that Oct4 inherently carries a protein transduction domain, which can translocate into human and mouse cells.A 16 amino acid peptide representing the third helix of the human Oct4 homeodomain, referred to as Oct4 protein transduction domain (Oct4-PTD), can internalize in mammalian cells upon conjugation to a fluorescence moiety thereby acting as a cell penetrating peptide (CPP). The cellular distribution of Oct4-PTD shows diffuse cytosolic and nuclear staining, whereas penetratin is strictly localized to a punctuate pattern in the cytoplasm. By using a Cre/loxP-based reporter system, we show that this peptide also drives translocation of a functionally active Oct4-PTD-Cre-fusion protein. We further provide evidence for translocation of full length Oct4 into human and mouse cell lines without the addition of any kind of cationic fusion tag. Finally, physico-chemical properties of the novel CPP are characterized, showing that in contrast to penetratin a helical structure of Oct4-PTD is only observed if the FITC label is present on the N-terminus of the peptide.Oct4 is a key transcription factor in stem cell research and cellular reprogramming. Since it has been shown that recombinant Oct4 fused to a cationic fusion tag can drive generation of iPSCs, our finding might contribute to further development of protein-based methods to generate iPSCs.Moreover, our data support the idea that transcription factors might be part of an alternative paracrine signalling pathway, where the proteins are transferred to neighbouring cells thereby actively changing the behaviour of the recipient cell.
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- Malmqvist, Robert, et al.
(författare)
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A K-Band RF-MEMS-Enabled Reconfigurable and Multifunctional Low-Noise Amplifier Hybrid Circuit
- 2011
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Ingår i: Active and Passive Electronic Components. - : Hindawi Publishing Corporation. - 0882-7516 .- 1563-5031. ; 2011, s. 284767-
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Tidskriftsartikel (refereegranskat)abstract
- A K-band (18–26.5 GHz) RF-MEMS-enabled reconfigurable and multifunctional dual-path LNA hybrid circuit (optimised for lowest/highest possible noise figure/linearity, resp.) is presented, together with its subcircuit parts. The two MEMS-switched low-NF (higher gain) and high-linearity (lower gain) LNA circuits (paths) present 16.0 dB/8.2 dB, 2.8 dB/4.9 dB and 15 dBm/20 dBm of small-signal gain, noise figure, and 1 dB compression point at 24 GHz, respectively. Compared with the two (fixed) LNA subcircuits used within this design, the MEMS-switched LNA circuit functions show minimum 0.6–1.3 dB higher NF together with similar values ofP1 dBat 18–25 GHz. The gain of one LNA circuit path is reduced by 25–30 dB when the MEMS switch and active circuitry used within in the same switching branch are switched off to select the other LNA path and minimise power consumption.
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- Marin-Soler, Adoracion, et al.
(författare)
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Sample Selection Algorithms for Enhanced MIMO Antenna Measurements Using Mode-Stirred Reverberation Chambers
- 2012
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Ingår i: IEEE Transactions on Antennas and Propagation. - 0018-926X .- 1558-2221. ; 60:8, s. 3892-3900
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Tidskriftsartikel (refereegranskat)abstract
- Mode-stirred reverberation chambers (MSRCs) are a useful tool for measuring several wireless-related MIMO antenna parameters. In a conventional single-cavity MSRC, the emulated fading environment is isotropic and the amplitude of the signal is Rayleigh distributed. Previous contributions have enhanced the emulation capabilities of MSRCs so as to include the ability to emulate Rician- and non-isotropic fading environments. In this contribution, arbitrary amplitude probability density functions (PDF) emulation using a MSRC is presented by selecting parts of the sample set that forms different statistical ensembles. Several algorithms are presented and compared in terms of computation time and power accuracy using simulated as well as measured data from different MSRCs to obtain Rician, on-body and amplitude PDFs of standardized models. The technique is patent-protected by EMITE.
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- Pettersson, L, et al.
(författare)
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Compact integrated slot array antennas for the 79 GHz automotive band
- 2010
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Ingår i: International Journal of Microwave Wireless Technologies. ; 2:3, s. 305-
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents two compact slot array antennas for the 79 GHz automotive bintegrated into a 28 µm thick benzocyclobutene (BCB) substrate attached to a 325 µm thick 5€“20 Ω cm bulk resistivity silicon wafer. The two antennas are a transmit (TX) 1 × 8 slot array antenna with a size of 1 × 23 mm a receive (RX) 8 × 8 slot array antenna with a size of 15 × 23 mm. Promising performance has been measured with the TX RX subarray antennas (gain >4 dBi) with good matching to 50 „¦ (RL > 10 dB) in the frequency range 70€“90 GHz. By using a metal cavity, mounted on the back of the antenna, potential parallel plate or cavity modes could be reduced the gain could also be increased by 2 dB. The measured the simulated results are consistent._x000D_
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