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- Morrell, Jane, et al.
(författare)
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Practical applications of sperm selection techniques for improving reproductive efficiency
- 2016
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Ingår i: Animal Reproduction. - 1806-9614 .- 1984-3143. ; 13, s. 340-345
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Konferensbidrag (refereegranskat)abstract
- Several selection techniques are available for processing spermatozoa. Apart from sperm washing to remove seminal plasma, only "swim-up" and colloid centrifugation have been used to any extent to prepare spermatozoa for in vitro fertilization, and only colloid centrifugation has been used to prepare sperm samples for artificial insemination. Single-layer centrifugation (SLC) through a species-specific colloid has been shown to be effective in selecting spermatozoa with good motility, normal morphology and intact chromatin in a range of species. This method is less time-consuming than swim-up, and has been scaled-up to allow whole ejaculates to be processed in a practical manner. The applications of SLC are as follows: to improve sperm quality in insemination doses or in samples for in vitro fertilization, to increase the shelf life of normal sperm doses, to remove pathogens ( viruses, bacteria), to improve cryosurvival by removing dead and dying spermatozoa before freezing or after thawing, to select spermatozoa for intracytoplasmic sperm injection, and to aid conservation breeding.
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- Sabés-Alsina, Maria, et al.
(författare)
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Heat stress has an effect on motility and metabolic activity of rabbit spermatozoa
- 2016
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Ingår i: Animal Reproduction Science. - : Elsevier BV. - 0378-4320 .- 1873-2232. ; 173, s. 18-23
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Tidskriftsartikel (refereegranskat)abstract
- In the warm months the function of the spermatozoa can be affected by the temperature of the reproductive tract of the female exposed to hyperthermic conditions. The aim of this study was to evaluate the impact of heat stress on sperm parameters in an in vitro model and to determine if there were seasonal effects on sperm heat tolerance. Sperm samples from 32 New Zealand White rabbits were collected in two seasons and incubated at scrotal (32.5 degrees C), body (37 degrees C) or hyperthermic (42 degrees C) temperatures for 3 h. Sperm viability and morphology were evaluated using nigrosin-eosin staining. Motility and metabolic activity parameters were determined using computer-assisted sperm analysis and the QBlue cell viability test, respectively. The incubation of spermatozoa at 42 degrees C decreased (P<0.05) the mean values of total motility, curvilinear (VCL) and mean velocity (VAP) as well as the metabolic activity with respect to the incubation at 32.5 degrees C and 37 degrees C.No seasonal effects were observed except for the highest percentages of bent and coiled tails in the cold season, and the highest mean values of VCL, linear velocity and VAP in the warm season (P<0.01). The interaction between in vitro heat stress and season was significant for metabolic activity (P=0.02). Our results suggest that rabbit spermatozoa parameters are largely modified by a short exposure to hyperthermic conditions, in terms of metabolic activity and motility parameters. Thus, a short exposure of spermatozoa to an environment of 42 degrees C in temperature for only 3 h may compromise sperm functionality. Additionally, sperm metabolic activity is influenced by season..
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