| 1. |
- Engstrom, P.E., et al.
(författare)
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Electrically mediated drug delivery for treatment of an adenocarcinoma transplanted into rat liver
- 2001
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Ingår i: Anticancer research. - 1791-7530. ; 21:3B, s. 1817-1822
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: In this study, electrochemotherapy (ECT), i.e. tumour treatment based on local augmentation of intracellular drug delivery from short, intense electric pulses, was evaluated in rats with an adenocarcinoma implanted into the liver. Tumour response and concentrations of macrophages and T-lymphocytes (CD4 and CD8) in and around the tumour were measured.MATERIALS AND METHODS: Rats were treated with permeabilizing electric pulses, bleomycin, or both, eight days after implantation of the tumour, while one group received sham treatment.RESULTS: Treatment with electric pulses and bleomycin resulted in a significantly reduced lesion volume and 92% cure rate (12 out of 13, p<0.0002 compared to the other treatment groups). The highest concentration of CD8 lymphocytes was found in tumours treated with electric pulses and bleomycin. Macrophages were found mainly in tumours treated with electric pulses, with or without bleomycin.CONCLUSION: Electrochemotherapy using millisecond exponential pulses and bleomycin is efficient in a rat liver tumour model and appears to stimulate the host's immune system.
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| 2. |
- Engström, P, et al.
(författare)
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Distribution of bleomycin in a rat model
- 2000
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Ingår i: Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery : Electrically Mediated Delivery of Molecules to Cells - Electrically Mediated Delivery of Molecules to Cells. - New Jersey : Humana Press. - 9780896036062 - 9781592590803 ; 37, s. 92-285
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Bokkapitel (refereegranskat)abstract
- Bleomycin has, in the years of developing electrochemotherapy (ECT), proven to be an extremely potent drug for this cancer treatment modality and is also the most frequently applied chemical agent. It is of importance to investigate the pharmacokinetics of bleomycin under normal conditions and particularly in combination with ECT.
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| 3. |
- Engström, P E, et al.
(författare)
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A new antitumour treatment combining radiation and electric pulses
- 2001
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Ingår i: Anticancer research. - 0250-7005. ; 21:3B, s. 15-1809
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Tidskriftsartikel (refereegranskat)abstract
- AIM: To investigate the antitumour effect of radiation in combination with electropermeabilization on subcutaneous rat glioma tumours.MATERIALS AND METHODS: Sub-optimal radiation treatment was administered separately or in combination with electric pulses of high voltage to subcutaneous rat brain tumours. The treatment was repeated on four consecutive days and evaluated by TGD and microscopical examination. The tumours were stained for Factor VlII/von Willebrand Factor to investigate the effects on the tumour vasculature.RESULTS: Radiation and electric pulses applied concomitantly resulted in a cure rate of 67% (tumour free >80 days after treatment). Radiation-treated animals showed progressive disease. Histological and immunohistochemical examination of electric impulse-treated tumours showed instant and severe deteriorating effects on tumour vasculature.CONCLUSION: A distinct antitumour effect of the combined treatment of electric pulses and radiation treatment was observed. We believe that the tumouricidal effect arises from destruction of the tumour vasculature but also from DNA related damage from reactive oxygen formed by the electric pulses and the radiation treatment.
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| 4. |
- Salford, L G, et al.
(författare)
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Treatment of rat glioma with electrochemotherapy
- 2000
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Ingår i: Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery : Electrically Mediated Delivery of Molecules to Cells - Electrically Mediated Delivery of Molecules to Cells. - New Jersey : Humana Press. - 9780896036062 - 9781592590803 ; 37, s. 7-313
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Bokkapitel (refereegranskat)abstract
- The first attempt to apply electrochemotherapy (ECT) to the brain was reported in 1993 by Salford et al. 1993 (1). They managed to significantly prolong the survival of RG2 glioma bearing Fischer-344 rats by 200% by iv administration of bleomycin followed by intracranial electrochemotherapy with exponential decaying pulses. In collaboration with the department of tumor immunology, Lund University, an ethyl-nitroso-urea induced rat glioma cell line (N32) is developed that produces glioma of malignant astrocytoma type with only half the growth rate of the RG2 cells (2). The N32 tumor implanted in rats was treated with intracranial electrochemotherapy and enhanced uptake of [111In]bleomycin from intracranial ECT was also demonstrated with a scintillation camera (3). (111)In-labeled bleomycin has been used to investigate the uptake and retention after ECT treatment of subcutaneous N32 tumors (4).
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