| 1. |
- Sandler, H, et al.
(författare)
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Antiserum-induced neutropenia in the rat : characterization of a rabbit anti-rat neutrophil serum.
- 1987
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Ingår i: British journal of experimental pathology. - 0007-1021. ; 68:1, s. 71-80
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Tidskriftsartikel (refereegranskat)abstract
- A heterologous rabbit anti-rat neutrophil serum (ANS) based on peptone-stimulated peritoneal exudate neutrophils (PMNLs) from Sprague Dawley rats was prepared. Leucoagglutination and indirect immunofluorescence assays revealed high titres of antibodies to rat PMNLs (1/2560), lower titre of antibodies to rat lymphocytes (1/160) and a very low titre against rat platelets (1/20). ANS given intravenously (i.v.) to rats in doses of up to 42 mg of protein/kg b.w. caused transient neutropenia, lasting about 10 min after administration, and thrombocytopenia, lasting about 5 min. Two minutes after an i.v. injection of 21 mg of ANS/kg b.w. there was profound uptake of 51Cr-labelled PMNLs in the lung, increased release of 51Cr to plasma, an increased amount of 51Cr in the spleen and consumption of greater than 98% of total complement (CH50). Two hours later there was high activity of 51Cr in the plasma, spleen and liver, while lung radioactivity had decreased to below baseline and CH50 had recovered to 55% of baseline. An intraperitoneal (i.p.) injection of ANS was followed by prolonged neutropenia with a maximum after 12 h. Simultaneously peripheral mononuclear cells slightly decreased. There was no change in the number of peripheral platelets in the blood or in the plasma concentration of fibrinogen, alpha 2-antiplasmin, plasminogen or plasminogen activators. Intraperitoneal administration of ANS did not affect CH50. It was concluded that the raised ANS had good specificity against rat PMNLs and was able to induce prolonged neutropenia after i.p. injection without affecting the complement of fibrinolytic system.
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| 2. |
- Sandler, H, et al.
(författare)
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Blockade of histamine receptors and thrombin-induced microembolic pulmonary edema in the rat.
- 1985
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Ingår i: Microcirculation, endothelium, and lymphatics. - 0740-9451. ; 2:4, s. 443-54
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Tidskriftsartikel (refereegranskat)abstract
- Pulmonary edema was induced by an intravenous infusion of bovine thrombin, 500 NIH/kg b.w., given in 5 min, in rats in which fibrinolysis had been inhibited by an intraperitoneal injection of the fibrinolysis inhibitor AMCA. Ninety minutes after termination of the thrombin infusion the lung weight was increased from 1.09 +/- 0.07 to 3.14 +/- 0.12 g due to edema. A high albumin concentration in the extravascular water indicated that the edema was a consequence of increased permeability in the microcirculation. In rats injected with the H1 histamine receptor antagonist mepyramine maleate and H2 receptor antagonist cimetidine after the thrombin infusion, the lung weight was significantly lower at 90 min (2.68 +/- 0.33 g), and the amount of edema as calculated morphometrically was slightly but significantly reduced. The concentration of albumin in the extravascular water (i.e. in the edema fluid) in rats subjected to histamine receptor blockade was unchanged, indicating that the slight decrease in edema was a result of decreased filtration pressure and not of an effect on microvascular permeability.
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| 3. |
- Sandler, H, et al.
(författare)
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Studies on the role of thromboxane in thrombin-induced pulmonary insufficiency in the rat.
- 1986
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Ingår i: Thrombosis Research. - 0049-3848 .- 1879-2472. ; 42:2, s. 165-75
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Tidskriftsartikel (refereegranskat)abstract
- During infusion of thrombin in rats pulmonary arterial pressure rose from 15 +/- 2 to 35 +/- 3 mmHg and mean arterial pressure fell from 120 +/- 6 to 49 +/- 27 mmHg. Plasma thromboxane B2 (TxB2) increased from 0.3 +/- 0.04 to 3.6 +/- 0.5 ng/ml. Ninety minutes later the lung weight and albumin concentration in the lung were increased (2.21 +/- 0.13 g and 22.7 +/- 4.7 mg/g) compared with controls (1.12 +/- 0.14 g and 8.5 +/- 0.9 mg/g). An inhibitor of thromboxane synthetase, Dazoxiben R, reduced the elevated pulmonary arterial pressure and the elevated plasma TxB2 concentration following infusion of thrombin. Ninety minutes after infusion of thrombin, the in vitro synthesis of TxB2 in lung tissue was increased. Dazoxiben and antineutrophil serum reduced this synthesis of TxB2 in vitro. The lung weight (2.18 +/- 0.20 g) and lung albumin concentration (21.4 +/- 3.4 mg/g) was not affected by Dazoxiben. The results indicate that TxA2 is an important mediator of the pressure changes in the early phase after infusion of thrombin and that neutrophils are associated with thromboxane formation in the lung tissue.
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