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Sökning: WFRF:(Sandström P.) > (2000-2004)

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  • Karlsson, J, 1966-, et al. (författare)
  • Sequencing of the Francisella tularensis strain Schu 4 genome reveals the shikimate and purine metabolic pathways, targets for the construction of a rationally attenuated auxotrophic vaccine.
  • 2000
  • Ingår i: Microbial & Comparative Genomics. - : Mary Ann Liebert Inc. - 1090-6592 .- 2168-6637. ; 5:1, s. 25-39
  • Tidskriftsartikel (refereegranskat)abstract
    • Francisella tularensis is the etiological agent of tularemia, a serious disease in several Northern hemisphere countries. The organism has fastidious growth requirements and is very poorly understood at the genetic and molecular levels. Given the lack of data on this organism, we undertook the sample sequencing of its genome. A random library of DNA fragments from a highly virulent strain (Schu 4) of F. tularensis was constructed and the nucleotide sequences of 13,904 cloned fragments were determined and assembled into 353 contigs. A total of 1.83 Mb of nucleotide sequence was obtained that had a G+C content of 33.2%. Genes located on plasmids pOM1 and pNFL10, which had been previously isolated from low virulence strains of F. tularensis, were absent but all of the other known F. tularensis genes were represented in the assembled data. F. tularensis Schu4 was able to grow in the absence of aromatic amino acids and orthologues of genes which could encode enzymes in the shikimate pathway in other bacteria were identified in the assembled data. Genes that could encode all of the enzymes in the purine biosynthetic and most of the en- zymes in the purine salvage pathways were also identified. This data will be used to develop defined rationally attenuated mutants of F. tularensis, which could be used as replacements for the existing genetically undefined live vaccine strain.
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  • Olafsson, P., et al. (författare)
  • Calculations of electrical resistivity for Al-Cuand Al-Mg-Sialloys
  • 2001
  • Ingår i: Materials Science and Technology. - : Maney Publishing. - 0267-0836 .- 1743-2847. ; 17:6, s. 655-662
  • Tidskriftsartikel (refereegranskat)abstract
    • A system for thermodynamical calculations (Thermo-Calc) was used to derive the solid solubility of the alloying elements in commercial Al-Cu and Al-Mg-Si alloys. The electrical resistivity was then calculated using a model developed by the authors based on the Matthiessen's rule. The calculated resistivity agreed with the observed resistivity within +/-2.5 n Omegam for the Al-Mg-Si alloys and +/- 2n Omegam for the Al-Cu alloys, except for Al-Mg-Si alloys containing boron or chromium and AI-Cu alloys with special compositions. MST/3725.
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10.
  • Sandström, J.P., et al. (författare)
  • Amino acid budgets in three aphid species using the same host plant
  • 2001
  • Ingår i: PHYSIOLOGICAL ENTOMOLOGY. - : BLACKWELL SCIENCE LTD. - 0307-6962. ; 26:3, s. 202-211
  • Tidskriftsartikel (refereegranskat)abstract
    • Nutrient provisioning in aphids depends both on the composition of ingested phloem sap and on the biosynthetic capabilities of the aphid and its intracellular symbionts. Amino acid budgets for three aphid species, Rhopalosiphum padi (L.), Schizaphis grami
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