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Purification and ch...
Purification and characterization of murine MZ and T2-MZP cells
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Rosado, M. M. (författare)
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- Aranburu, Alaitz (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
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Scarsella, M. (författare)
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Cascioli, S. (författare)
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Giorda, E. (författare)
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Carsetti, R. (författare)
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(creator_code:org_t)
- 2021-01-22
- 2021
- Engelska.
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Ingår i: Regulatory B Cells. Methods in Molecular Biology, vol 2270. Mion F., Tonon S. (eds). - New York, NY : Springer. - 1064-3745. - 9781071612361 ; , s. 3-25
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The spleen is the second major reservoir of B cells in the adult. In the spleen, cells, generated in the bone marrow, are selected, mature, and become part of the peripheral B-cell pool. Murine spleen comprises several B-cell subsets representing various maturation stages and/or cell functions. The spleen is a complex lymphoid organ organized into two main structures with different functions: the red and white pulp. The red pulp is flowed with blood while the white pulp is organized in primary follicles, with a B-cell area composed of follicular B cells and a T-cell area surrounding a periarterial lymphatic sheath. The frontier between the red and white pulp is defined as the marginal zone (MZ) and contains the MZ B cells. Because B cells, localized in different areas, are characterized by distinct expression levels of B-cell receptor (BCR) and of other surface markers, splenic B-cell subsets can be easily identified and purified by flow cytometry analyses and fluorescence-activated cell sorting (FACS). Here, we will focus on MZ B cells and on their precursors, giving some experimental hints to identify, generate, and isolate these cells. We will combine the use of FACS analysis and confocal microscopy to visualize MZ B cells in cell suspensions and in tissue sections, respectively. We will also give some clues to analyze B-cell repertoire on isolated MZ-B cells. © Springer Science+Business Media, LLC, part of Springer Nature 2021.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- Flow cytometry
- Heavy chain repertoire
- Heavy chain sequencing
- Marginal zone B cells
- Spleen
- Transitional B cells
Publikations- och innehållstyp
- vet (ämneskategori)
- kap (ämneskategori)
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