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- Malacrinò, Antonino, et al.
(författare)
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A Metabarcoding Survey on the Fungal Microbiota Associated to the Olive Fruit Fly
- 2017
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Ingår i: Microbial Ecology. - : SPRINGER. - 0095-3628 .- 1432-184X. ; 73:3, s. 677-684
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Tidskriftsartikel (refereegranskat)abstract
- The occurrence of interaction between insects and fungi is interesting from an ecological point of view, particularly when these interactions involve insect pests and plant pathogens within an agroecosystem. In this study, we aimed to perform an accurate analysis on the fungal microbiota associated to Bactrocera oleae (Rossi) through a metabarcoding approach based on 454 pyrosequencing. From this analysis, we retrieved 43,549 reads that clustered into 128 operational taxonomic units (OTUs), of which 29 resulted in the “core” associate fungi of B. oleae. This fungal communitywasmainly represented by sooty mould fungi, such as Cladosporium spp., Alternaria spp. and Aureobasidium spp., by plant pathogens like Colletotrichum spp. and Pseudocercospora spp., along with several other less abundant taxa whose ecology is unclear in most of the cases. Our findings lead to new insights into the microbial ecology of this specific ecological niche, enabling the understanding of a complex network of interactions within the olive agroecosystem.
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| 2. |
- Malacrinó, Antonino, et al.
(författare)
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Fungal communities associated with bark and ambrosia beetles trapped at international harbours
- 2017
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Ingår i: Fungal ecology. - : Elsevier. - 1754-5048 .- 1878-0083. ; 28, s. 44-52
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Tidskriftsartikel (refereegranskat)abstract
- Bark and ambrosia beetles (Coleoptera; Scolytinae) establish trophic relationships with fungi, which could be also agents of plant diseases. Orthotomicus erosus (Wollaston) and Xyleborinus saxesenii (Ratzeburg) are two species of Palaearctic origin that have been introduced in several countries around the world. Here, we investigated their associated fungal communities using individuals trapped at harbours in their native range, without strictly focusing on nutritional symbionts. Targeting the ITS2 region of the fungal rDNA through pyrosequencing, we retrieved taxa known to be agents of plant diseases, taxa never previously reported associated with these beetle species, and sequence clusters not linked to any known fungus. These findings underline that surveillance at harbours should be extended to the fungi associated with trapped bark and ambrosia beetles, taking into account their role as potential vectors of plant pathogens. (C) 2017 Elsevier Ltd and British Mycological Society. All rights reserved.
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