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| 2. |
- Kohrgruber, N, et al.
(författare)
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Survival, maturation, and function of CD11c- and CD11c+ peripheral blood dendritic cells are differentially regulated by cytokines
- 1999
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Ingår i: Journal of Immunology. - : American association of immunologists. - 0022-1767 .- 1550-6606. ; 163:6, s. 3250-3259
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Tidskriftsartikel (refereegranskat)abstract
- Two types of dendritic cells (DC) are circulating in human blood and can be identified by their differential expression of the myeloid Ag CD11c. In this study, we show that CD11c- peripheral blood (PB)-DC correspond to plasmacytoid DC of lymphoid tissue not only by their surface Ag expression profile but, more impressively, by their peculiar ultramorphology. We also demonstrate that CD11c- and CD11c+ DC differ in the quality of their response to and in their requirement for certain cytokines. Freshly isolated CD11c- cells depend on IL-3 for survival and use autocrine or exogenous TNF-alpha as maturation signal, leading to the appearance of a highly dendritic phenotype, the up-regulation and redistribution of MHC class II from lysosomal compartments to the plasma membrane, the increased expression of costimulatory molecules, and the switch from a high Ag-processing to a low Ag-processing/potent accessory cell mode. Surprisingly, IL-4 efficiently killed freshly isolated CD11c- PB-DC, but did not impair the viability of CD11c+ PB-DC and, together with GM-CSF, induced maturation of these cells. A direct functional comparison revealed that neo-Ag-modified and subsequently matured CD11c- but to a lesser extent CD11c+ DC were able to prime naive Ag-specific CD4+ T cells. Our findings show that two diverse DC types respond to certain T cell-derived cytokines in a differential manner and, thus, suggest that suppression or activation of functionally diverse DC types may be a novel mechanism for the regulation of the quantity and quality of immune responses.
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| 3. |
- Maurer, D, et al.
(författare)
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Peripheral blood dendritic cells express Fc epsilon RI as a complex composed of Fc epsilon RI alpha- and Fc epsilon RI gamma-chains and can use this receptor for IgE-mediated allergen presentation
- 1996
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Ingår i: Journal of Immunology. - : American association of immunologists. - 0022-1767 .- 1550-6606. ; 157:2, s. 607-616
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Tidskriftsartikel (refereegranskat)abstract
- Originally limited to basophils and mast cells, the spectrum of high affinity IgE receptor (Fc epsilon RI-bearing cells has expanded recently to include Langerhans cells, dermal dendritic cells (DC), monocytes, and eosinophils. As a result of studies on the distribution, structure, and function of Fc epsilon RI on APCs, we discovered a minor nonbasophil, nonmonocyte PBMC population that can bind IgE via Fc epsilon RI. This receptor occurs on the surface of these cells as a multimeric structure containing Fc epsilon RI alpha- and Fc epsilon RI gamma-chains but, unlike its counterpart on basophils, lacking Fc epsilon RI beta. Further experiments revealed that these Fc epsilon RI alpha gamma-expressing cells closely resemble peripheral blood DC by immunophenotype (HLA-DRhigh, HLA-DQhhigh; CD4+, CD11a+, CD32+, CD33+, B7/2 (CD86)+; CD11blow, CD14low, CD40low, CD54low, CD64low) and cell morphology. These features allowed us to isolate Fc epsilon RI-expressing DC from the peripheral blood and to investigate their immunostimulatory properties. We found Fc epsilon RI-positive DC to be efficient stimulators of both primary (allogeneic MLR) and Fc epsilon RI/IgE-dependent, secondary T cell responses at low cell numbers. Thus, Fc epsilon RI-expressing DC may not only amplify established type I allergic immune reactions but, unlike Fc epsilon RI-positive semiprofessional APCs, may be able to prime naive T cells to common and/or cryptic epitopes of IgE-reactive Ags.
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| 4. |
- Evangelista, C, et al.
(författare)
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Measurement of the (p)over-barp->KsKs reaction for 0.6 to 1.9 GeV/c
- 1997
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Ingår i: PHYSICAL REVIEW D. - : AMER INST PHYSICS. - 0556-2821. ; 56:7, s. 3803-3810
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Tidskriftsartikel (refereegranskat)abstract
- The <(p)over bar p> --> KSKS --> 4 pi(+/-) cross section was measured at incident antiproton momenta between 0.6 and 1.9 GeV/c using the CERN Low Energy Antiproton Ring. This investigation was part of a systematic study of in-flight antiproton-proton anni
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| 5. |
- Evangelista, C, et al.
(författare)
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Study of the reaction (p)over-bar-p ->phi phi from 1.1 to 2.0 GeV/c
- 1998
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Ingår i: PHYSICAL REVIEW D. - : AMERICAN PHYSICAL SOC. - 0556-2821. ; 57:9, s. 5370-5381
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Tidskriftsartikel (refereegranskat)abstract
- A study has been performed of the reaction <(p)over bar p>-->4K(+/-) using in-flight antiprotons from 1.1 to 2.0 GeV/c incident momentum interacting with a hydrogen jet target. The reaction is dominated by the production of a pair of phi mesons. The <(p)o
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