| 1. |
- Buonaguro, L, et al.
(författare)
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DNA-VLP prime-boost intra-nasal immunization induces cellular and humoral anti-HIV-1 systemic and mucosal immunity with cross-clade neutralizing activity
- 2007
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Ingår i: Vaccine. - : Elsevier BV. - 0264-410X .- 1873-2518. ; 25:32, s. 5968-5977
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Tidskriftsartikel (refereegranskat)abstract
- The immune response to HIV-1 virus-like particles (VLPs), presenting a clade A Ugandan gp120, has been evaluated in a mouse model by intra-nasal (i.n.) administration by a VLP + VLP homologous or a DNA + VLP heterologous prime-boost immunization protocol, including a HIV-1 DNA gp160/rev plasmid. Furthermore, the effect of the Eurocine lipid-based mucosal L3 adjuvant on the VLP immunogenicity has been assessed as well. The designed heterologous protocol is able to increase the env-specific humoral and cellular immune response, compared to the homologous protocol, which is to some extent increased by the administration of L3-adjuvanted VLP boosting dose. The anti-gag response is statistically increased in both homologous and heterologous protocols, particularly when the VLP boosting dose is adjuvanted. Immune sera from immunized animals exhibit >50% ex vivo neutralizing activity against heterologous A and B-clade viral isolates. An envelope B-cell epitope mapping shows an enhanced response against V3 epitopes all across the C2-V5 region in the heterologous prime-boost immunization strategy. The induction of humoral immunity at mucosal sites, which represents the main port of entry for the HIV-1 infection, is extremely relevant. In this framework, the DNA-VLP heterologous prime-boost protocol appears a promising preventive vaccine approach which can significantly benefit from specific mucosal adjuvants, as the Eurocine L3. © 2007 Elsevier Ltd. All rights reserved.
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| 4. |
- Cooper, V.R., et al.
(författare)
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Stacking Interactions and the Twist of DNA
- 2008
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Ingår i: Journal of the American Chemical Society. ; 130, s. 1304-
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Tidskriftsartikel (refereegranskat)abstract
- The importance of stacking interactions for the Twist and stability of DNA is investigated using the fully ab initio van der Waals density functional (vdW-DF). Our results highlight the role that binary interactions between adjacent sets of base pairs play in defining the sequence-dependent Twists observed in high-resolution experiments. Furthermore, they demonstrate that additional stability gained by the presence of thymine is due to methyl interactions with neighboring bases, thus adding to our understanding of the mechanisms that contribute to the relative stability of DNA and RNA. Our mapping of the energy required to twist each of the 10 unique base pair steps should provide valuable information for future studies of nucleic acid stability and dynamics. The method introduced will enable the nonempirical theoretical study of significantly larger pieces of DNA or DNA/amino acid complexes than previously possible.
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| 7. |
- Langreth, D. C., et al.
(författare)
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A density functional for sparse matter
- 2009
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Ingår i: Journal of Physics Condensed Matter. - : IOP Publishing. - 0953-8984 .- 1361-648X. ; 21:8, s. 084203-
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Tidskriftsartikel (refereegranskat)abstract
- Sparse matter is abundant and has both strong local bonds and weak nonbonding forces, in particular nonlocal van der Waals (vdW) forces between atoms separated by empty space. It encompasses a broad spectrum of systems, like soft matter, adsorption systems and biostructures. Density-functional theory (DFT), long since proven successful for dense matter, seems now to have come to a point, where useful extensions to sparse matter are available. In particular, a functional form, vdW-DF (Dion et al 2004 Phys. Rev. Lett. 92 246401; Thonhauser et al 2007 Phys. Rev. B 76 125112), has been proposed for the nonlocal correlations between electrons and applied to various relevant molecules and materials, including to those layered systems like graphite, boron nitride and molybdenum sulfide, to dimers of benzene, polycyclic aromatic hydrocarbons (PAHs), doped benzene, cytosine and DNA base pairs, to nonbonding forces in molecules, to adsorbed molecules, like benzene, naphthalene, phenol and adenine on graphite, alumina and metals, to polymer and carbon nanotube (CNT) crystals, and hydrogen storage in graphite and metal–organic frameworks (MOFs), and to the structure of DNA and of DNA with intercalators. Comparison with results from wavefunction calculations for the smaller systems and with experimental data for the extended ones show the vdW-DF path to be promising. This could have great ramifications.
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| 8. |
- Langreth, D. C., et al.
(författare)
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Van der Waals Density Functional Theory with Applications
- 2005
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Ingår i: International Journal of Quantum Chemistry. - : Wiley. - 0020-7608 .- 1097-461X. ; 101:5, s. 599-610
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Tidskriftsartikel (refereegranskat)abstract
- The details of a density functional that includes van der Waals (vdW) interactions are presented. In particular we give some key steps of the transition from a form for fully planar systems to a procedure for realistic layered compounds that have planar symmetry only on large-distance scales, and which have strong covalent bonds within the layers. It is shown that the random-phase approximation of that original functional can be replaced by an approximation that is exact at large separation between vdW interacting fragments and seamless as the fragments merge. An approximation to the latter which renders the functional easily applicable and which preserves useful accuracy in both limits and in between is given. We report additional data from applications to forms of graphite, boron nitride, and molybdenum sulfide not reported in our previous communication.(C) 2004 Wiley Periodicals, Inc.
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| 9. |
- Morrow, S A, et al.
(författare)
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High-resolution study of the C-12(gamma,p gamma')B-11 reaction using a HpGe detector to resolve excited states of B-11 through the observation of their gamma-ray decays
- 2006
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Ingår i: Physical Review C: covering nuclear physics. ; 73
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Tidskriftsartikel (refereegranskat)abstract
- Relative populations of states in 11B following the 12C(,p)11B reaction have been measured with high resolution using a 70% HpGe detector to observe decay rays from the residual nucleus. The triplet of states near 7 MeV in 11B are resolved and the measured populations compared to previous data. The analysis includes a consideration of -proton angular correlations, which was not made in the previous measurement. The new and previous results corrected for angular correlation effects agree reasonably well with calculations that include one- and two-body nuclear currents, pion exchange, and currents, under the assumption that the photons are mainly absorbed on exchanged pions.
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| 10. |
- Reiter, A., et al.
(författare)
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Measurement and simulation of the neutron response of the Nordball liquid scintillator array
- 2006
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Ingår i: Nuclear Instruments & Methods in Physics Research. Section A: Accelerators, Spectrometers, Detectors, and Associated Equipment. - : Elsevier BV. - 0167-5087 .- 0168-9002. ; 565:2, s. 753-762
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Tidskriftsartikel (refereegranskat)abstract
- The response of the liquid scintillator array Nordball to neutrons in the energy range 1.5 < T-n < 10 MeV has been measured by time of flight using a Cf-252 fission source. Fission fragments were detected by means of a thin-film plastic scintillator. The measured differential and integral neutron detection efficiencies agree well with predictions of a Monte Carlo simulation of the detector which models geometry accurately and incorporates the measured, non-linear proton light output as a function of energy. The ability of the model to provide systematic corrections to photoneutron cross-sections, measured by Nordball at low energy, is tested in a measurement of the two-body deuteron photodisintegration cross-section in the range E-gamma = 14-18 MeV. After correction the present H-2 (gamma, n)p measurements agree well with a published evaluation of the large body of H-2(gamma, p)n data. (c) 2006 Elsevier B.V. All rights reserved.
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