| 1. |
- Fissum, Kevin, et al.
(författare)
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High-resolution measurement of the 12C(γ,p)11B reaction to excited states for Eγ=50–70MeV
- 1998
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Ingår i: Physical Review C: covering nuclear physics. - 2469-9985. ; 58:4, s. 2167-2173
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Tidskriftsartikel (refereegranskat)abstract
- Relative population of states in 11B following the 12C(γ,p) reaction has been measured with high resolution using the deexcitation γ-ray technique. The states near 7 MeV in 11B are clearly resolved and the measured population clarifies earlier conflicting data. Comparison of the results with new calculations indicates the importance of both one-nucleon and multinucleon processes.
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| 2. |
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| 3. |
- Sims, D. A., et al.
(författare)
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(γ,n) study of the isovector quadrupole resonance in 40Ca
- 1997
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Ingår i: Physical Review C: covering nuclear physics. - 2469-9985. ; 55:3, s. 1288-1294
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Tidskriftsartikel (refereegranskat)abstract
- The forward-to-backward asymmetry of neutrons emitted in the 40Ca(γ,n0) reaction was measured at photon energies in the range of 27–50 MeV. An energy-dependent asymmetry was observed that is interpreted as evidence of interference between the isovector quadrupole resonance and the giant dipole resonance. Data analysis in terms of semiclassical and direct-semidirect models estimate the isovector quadrupole resonance to be at an excitation energy of 31.0±1.5 MeV, with a width of 16.0±1.5 MeV, and exhausting most of the energy-weighted sum rule for the isovector quadrupole resonance.
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| 4. |
- Berndt, Kurt D, et al.
(författare)
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Designed replacement of an internal hydration water molecule in BPTI : structural and functional implications of a glycine-to-serine mutation.
- 1993
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Ingår i: Biochemistry. - : American Chemical Society (ACS). - 0006-2960 .- 1520-4995. ; 32, s. 4564-4570
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Tidskriftsartikel (refereegranskat)abstract
- The three-dimensional structure of the basic pancreatic trypsin inhibitor (BPTI) contains four internal water molecules, which form a total of nine intermolecular hydrogen bonds with the BPTI polypeptide chain. To investigate the effect of such internal hydration on protein structure and stability, we displaced one of the internal water molecules in a recombinant BPTI analogue, BPTI(G36S), in which Gly 36 is replaced by serine. The replacement of a water molecule by the seryl side chain was established by the absence of the protein-water nuclear Overhauser effects (NOE) that had been attributed to the water molecule near Gly 36 in wild-type BPTI and by the presence of new, intramolecular NOEs to the hydroxyl proton of Ser 36. BPTI(G36S) has slightly reduced thermal stability compared to BPTI, corresponding to a destabilization by delta (delta G) approximately 0.7 kcal/M in 6 M guanidinium hydrochloride solution. Additionally, the stabilities of the complexes formed between BPTI(G36S) and trypsin, plasmin, or kallikrein are significantly reduced when compared to the corresponding complexes with wild-type BPTI.
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| 5. |
- Johansson, Hugo, et al.
(författare)
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Herpes simplex type 1-induced Fc receptor binds to the Cgamma2-Cgamma3 interface region of IgG in the area that binds staphylococcal protein A
- 1989
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Ingår i: Immunology. - 0019-2805. ; 66:1, s. 8-13
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Tidskriftsartikel (refereegranskat)abstract
- The binding site of immunoglobulin G (IgG) to herpes simplex virus (HSV) type 1-induced Fc receptor was investigated using human IgG Fc intermediate (Fc(i)) fragments, fragment D of staphylococcal protein A (SPA) and chemically modified human IgG. Human IgG Fc(i) fragment composed of one Cgamma2 and two Cgamma3 domains, bound strongly to HSV-1-infected cells. Fragment D, a monovalent subunit of SPA, inhibited the binding of radiolabelled human IgG Fc fragments to the HSV Fc receptor. Reductively methylated human IgG reacted equally well to HSV-infected cells, as did chemically unmodified IgG in contrast to N-acetylimidazole-modified and diethylpyrocarbonate-modifed human IgG, which were unreactive. These results suggest a similar binding site on human IgG for SPA and the HSV-1 Fc receptor with involvement of the amino acid residues Tyr and His but not Lys. The similarities of binding sites on the IgG molecule for the HSV-1 Fc receptor and rheumatoid factors (RF) may be important for understanding the mechanism of RF production in rheumatoid arthritis or other disease states.
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| 6. |
- Nardella, F A, et al.
(författare)
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Fc epitopes for human rheumatoid factors and the relationships of rheumatoid factors to the Fc binding proteins of microorganisms
- 1988
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Ingår i: Scandinavian Journal of Rheumatology. Supplement. - : Informa UK Limited. - 1502-7740 .- 0300-9742 .- 1502-7732. ; 17:Suppl. 75, s. 190-198
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Tidskriftsartikel (refereegranskat)abstract
- Work from our laboratories has shown that the major antigenic determinants for rheumatoid factors (RFs) are in the C gamma 2-C gamma 3 interface region of IgG in the same area that binds staphylococcal protein A (SPA). Furthermore, the Fc binding proteins of groups A, C and G streptococci as well as the Fc binding proteins induced on cell surfaces by herpes simplex virus type I also bind to the same area of IgG. These binding site similarities between RFs and the microbial Fc binding proteins suggested conformational similarities between the RF antigen combining regions and the Fc binding regions of the microbial proteins. This hypothesis was supported by the observation that antibodies to SPA bind to the antigen combining regions of most RFs as well as to the Fc binding region of the T15 group A streptococcal Fc binding protein. These findings indicate that RFs bear the conformational internal image of these microbial proteins and suggest that RFs could arise as antibodies to the idiotypic determinants on antibodies to microbial Fc binding proteins. Alternatively, microbial Fc binding proteins could present IgG to the immune system in a way that renders specific areas of the C gamma 2-C gamma 3 interface region immunogenic. These relationships between RFs and microbial Fc binding proteins may prove to be important for our understanding of the generation of RFs in rheumatoid arthritis.
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| 7. |
- Ruijter, Emiel Th, et al.
(författare)
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Rapid microwave-stimulated fixation of entire prostatectomy specimens
- 1997
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Ingår i: Journal of Pathology. - 0022-3417. ; 183:3, s. 369-375
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Tidskriftsartikel (refereegranskat)abstract
- Conventional fixation of large solid surgical specimens is a slow process. Consequently, autolytic damage to tissues may occur if the fixative does not reach the central part of the specimen in time. However, as there is also a time relationship between formalin fixation and antigen masking, fixation for too long can also be detrimental. In seeking the optimum balance for fixation, microwave irradiation might be of assistance. This study set out to evaluate methods for fixing entire prostate glands within a brief period of time, using microwave-stimulated formalin fixation. The results show that entire prostates can be optimally fixed if formalin is present throughout the tissue as the temperature is increased by microwave irradiation. This is achieved by injecting the fixative into the prostate at multiple sites immediately following prostatectomy. The technique described ensures standardization of a critical step during tissue processing, leading to uniform microscopic results with both routine and immunohistochemical stains. It is a simple, rapid method, suitable for routine diagnostic use. Using this modified approach, DNA of much larger sizes can be extracted from paraffin-embedded material, which could expand the possibilities for molecular analysis.
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| 8. |
- Shi, L X, et al.
(författare)
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Characterisation of the PsbX protein from Photosystem II and light regulation of its gene expression in higher plants
- 1999
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Ingår i: Plant Molecular Biology. - 0167-4412 .- 1573-5028. ; 40:4, s. 737-744
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Tidskriftsartikel (refereegranskat)abstract
- The location and expression of the previously uncharacterised photosystem II subunit PsbX have been analysed in higher plants. We show that this protein is a component of photosystem II (PSII) core particles but absent from light-harvesting complexes or PSII reaction centres. PsbX is, however, localised to the near vicinity of the reaction centre because it can be cross-linked to cytochrome b559, which is known to be associated with the D1/D2 dimer. We also show that the expression of this protein is tightly regulated by light, since neither protein nor mRNA is found in dark-grown plants.
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| 9. |
- Sims, D. A., et al.
(författare)
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The He(γ,n) reaction: a potential testing ground for the alpha-particle wavefunction
- 1998
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Ingår i: Physics Letters B. - 0370-2693. ; 442:1-4, s. 43-47
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Tidskriftsartikel (refereegranskat)abstract
- Differential cross sections (σ(Eγ,θn)) for the He(γ,n) reaction have been measured at Eγ=50–71 MeV and θn=30–120°. These data are compared with theoretical predictions where a microscopic calculation of the He and He wavefunctions has been made within the Alt-Grassberger-Sandhas, integral-equation formalism.
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