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Multiple effects of...
Multiple effects of paclitaxel are modulated by a high c-myc amplification level.
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Bottone, Maria Grazia (författare)
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Soldani, Cristiana (författare)
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- Tognon, Gianluca, 1976 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine
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visa fler...
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Gorrini, Chiara (författare)
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Lazzè, M Claudia (författare)
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Brison, Olivier (författare)
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Ciomei, Marina (författare)
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Pellicciari, Carlo (författare)
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Scovassi, A Ivana (författare)
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visa färre...
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(creator_code:org_t)
- 2003
- 2003
- Engelska.
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Ingår i: Experimental cell research. - 0014-4827. ; 290:1, s. 49-59
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Paclitaxel affects microtubule stability by binding to beta-tubulin, thus leading to cell accumulation in the G(2)/M phase, polyploidization, and apoptosis. Because both cell proliferation and apoptosis could be somehow regulated by the protooncogene c-myc, in this work we have investigated whether the c-myc amplification level could modulate the multiple effects of paclitaxel. To this aim, paclitaxel was administered to SW613-12A1 and -B3 human colon carcinoma cell lines (which are characterized by a high and low c-myc endogenous amplification level, respectively), and to the B3mycC5 cell line, with an enforced exogenous expression of c-myc copies. In this experimental system, we previously demonstrated that a high endogenous/exogenous level of amplification of c-myc enhances serum deprivation- and DNA damage-induced apoptosis. Accordingly, the present results indicate that a high c-myc amplification level potentiates paclitaxel cytotoxicity, confers a multinucleated phenotype, and promotes apoptosis to a great extent, thus suggesting that c-myc expression level is relevant in modulating the cellular responses to paclitaxel. We have recently shown in HeLa cells that the phosphorylated form of c-Myc accumulates in the nucleus, as distinct nucleolar and extranucleolar spots; here, we demonstrated that, after the treatment with paclitaxel, phosphorylated c-Myc undergoes redistribution, becoming diffused in the nucleoplasm.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Nyckelord
- Antineoplastic Agents
- Phytogenic
- pharmacology
- Apoptosis
- drug effects
- genetics
- Carcinoma
- drug therapy
- genetics
- metabolism
- Cell Compartmentation
- drug effects
- physiology
- Cell Division
- drug effects
- genetics
- Cell Nucleus
- drug effects
- genetics
- metabolism
- Colonic Neoplasms
- drug therapy
- genetics
- metabolism
- Eukaryotic Cells
- drug effects
- metabolism
- Gene Amplification
- drug effects
- genetics
- Gene Dosage
- Genes
- myc
- drug effects
- physiology
- Humans
- Neoplasms
- drug therapy
- genetics
- metabolism
- Paclitaxel
- pharmacology
- Phosphorylation
- drug effects
- Tumor Cells
- Cultured
- Up-Regulation
- drug effects
- genetics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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