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- Amato, Alberto, et al.
(författare)
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Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi
- 2018
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Ingår i: ISME Journal. - : Springer Science and Business Media LLC. - 1751-7362 .- 1751-7370. ; 12, s. 1594-1604
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Tidskriftsartikel (refereegranskat)abstract
- Diatoms and copepods are main actors in marine food webs. The prey-predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affect the diatom prey beyond the direct effect of grazing. Here, we investigated the response of the chain-forming centric diatom Skeletonema marinoi to grazer cues, including changes in morphology, gene expression and metabolic profile. S. marinoi cells were incubated with Calanus finmarchicus or with Centropages typicus and in both cases responded by reducing the chain length, whereas changes in gene expression indicated an activation of stress response, changes in the lipid and nitrogen metabolism, in cell cycle regulation and in frustule formation. Transcripts linked to G protein-coupled receptors and to nitric oxide synthesis were differentially expressed suggesting involvement of these signalling transduction pathways in the response. Downregulation of a lipoxygenase in the transcriptomic data and of its products in the metabolomic data also indicate an involvement of oxylipins. Our data contribute to a better understanding of the gene function in diatoms, providing information on the nature of genes implicated in the interaction with grazers, a crucial process in marine ecosystems.
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- Betsou, F, et al.
(författare)
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Standard PREanalytical Code Version 3.0
- 2018
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Ingår i: Biopreservation and biobanking. - : Mary Ann Liebert Inc. - 1947-5543 .- 1947-5535. ; 16:1, s. 9-12
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Tidskriftsartikel (refereegranskat)
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- Selander, Erik, 1973, et al.
(författare)
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Hydrodynamic properties and distribution of bait downstream of a zooplankton trap
- 2017
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Ingår i: Journal of Plankton Research. - : Oxford University Press (OUP). - 0142-7873 .- 1464-3774. ; 39:6, s. 1020-1027
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Tidskriftsartikel (refereegranskat)abstract
- The flow regime around a chemically baited trap is crucial for the trapping process and distribution of bait downstream of traps. We measured the flow field downstream of a trap prototype in flume experiments and mapped the distribution of bait using laser induced fluorescence. The trap produced a downstream wake, where flow recirculated towards the trap, allowing organisms slower than the free stream flow to interact with the trap. The chemical tracer revealed an average gradient with increasing concentrations towards the trap. Finally, we evaluated trap performance in field experiments. Traps with internal light caught on average 3.4 times more zooplankton than traps without light in short-term deployments (1 h). Trapping efficiency could be manipulated by chemical stimuli; A piece of fish (Salmo salar) inside traps deterred 79% of the zooplankton compared to traps without fish. We conclude that the flow regime around a cylindrical trap may facilitate trapping and that combined stimuli modalities may allow higher selectivity. The effective radius of the trap will depend on the surrounding flow and will likely be small when flow-rate exceeds swimming speed of targeted organisms. Finally, we propose applications for selective traps in aquaculture and pest management.
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