Sökning: WFRF:(Shabestary Kiyan)
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Genetic and nutrien...
Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production
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- Anfelt, Josefine (författare)
- KTH,Proteomik och nanobioteknologi,Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)
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- Kaczmarzyk, Danuta (författare)
- KTH,Proteomik och nanobioteknologi,Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)
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- Shabestary, Kiyan (författare)
- KTH,Proteomik och nanobioteknologi,Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)
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- Renberg, Björn (författare)
- KTH,Proteomik och nanobioteknologi,Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)
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- Rockberg, Johan (författare)
- KTH,Proteomik och nanobioteknologi,Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)
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- Nielsen, Jens B, 1962 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Uhlén, Mathias (författare)
- KTH,Proteomik och nanobioteknologi,Tech Univ Denmark,Danmarks Tekniske Universitet,Technical University of Denmark,Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)
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- Hudson, Elton P. (författare)
- KTH,Proteomik och nanobioteknologi,Kungliga Tekniska Högskolan (KTH),Royal Institute of Technology (KTH)
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(creator_code:org_t)
- 2015-10-16
- 2015
- Engelska.
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Ingår i: Microbial Cell Factories. - : BioMed Central. - 1475-2859. ; 14
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Abstract
Ämnesord
Stäng
- Background: There is a strong interest in using photosynthetic cyanobacteria as production hosts for biofuels and chemicals. Recent work has shown the benefit of pathway engineering, enzyme tolerance, and co-factor usage for improving yields of fermentation products. Results: An n-butanol pathway was inserted into a Synechocystis mutant deficient in polyhydroxybutyrate synthesis. We found that nitrogen starvation increased specific butanol productivity up to threefold, but cessation of cell growth limited total n-butanol titers. Metabolite profiling showed that acetyl-CoA increased twofold during nitrogen starvation. Introduction of a phosphoketolase increased acetyl-CoA levels sixfold at nitrogen replete conditions and increased butanol titers from 22 to 37 mg/L at day 8. Flux balance analysis of photoautotrophic metabolism showed that a Calvin-Benson-Bassham-Phosphoketolase pathway had higher theoretical butanol productivity than CBB-Embden-Meyerhof-Parnas and a reduced butanol ATP demand. Conclusion: These results demonstrate that phosphoketolase overexpression and modulation of nitrogen levels are two attractive routes toward increased production of acetyl-CoA derived products in cyanobacteria and could be implemented with complementary metabolic engineering strategies.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- Biofuel
- Butanol
- Cyanobacteria
- Metabolic engineering
- Phosphoketolase
- Starvation
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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