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Inter-laboratory study on standardized MPS libraries : evaluation of performance, concordance, and sensitivity using mixtures and degraded DNA
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- Müller, Petra (författare)
- Medical University of Innsbruck
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- Sell, Christian (författare)
- Bundeskriminalamt (BKA)
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- Hadrys, Thorsten (författare)
- Bayerische Staatliche Polizei
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- Hedman, Johannes (författare)
- Lund University,Lunds universitet,Teknisk mikrobiologi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Applied Microbiology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Swedish National Forensic Center
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- Bredemeyer, Steffi (författare)
- Charité - University Medicine Berlin
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- Laurent, Francois Xavier (författare)
- Institut National de Police Scientifique
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- Roewer, Lutz (författare)
- Charité - University Medicine Berlin
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- Achtruth, Sabrina (författare)
- Landeskriminalamt Berlin, Kriminaltechnisches Institut
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- Sidstedt, Maja (författare)
- Lund University,Lunds universitet,Teknisk mikrobiologi,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Applied Microbiology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Swedish National Forensic Center
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- Sijen, Titia (författare)
- Netherlands Forensic Institute
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- Trimborn, Marc (författare)
- Landeskriminalamt Berlin, Kriminaltechnisches Institut
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- Weiler, Natalie (författare)
- Netherlands Forensic Institute
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- Willuweit, Sascha (författare)
- Charité - University Medicine Berlin
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- Bastisch, Ingo (författare)
- Bundeskriminalamt (BKA)
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- Parson, Walther (författare)
- Pennsylvania State University,Medical University of Innsbruck
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(creator_code:org_t)
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- 2019-11-19
- 2020
- Engelska 14 s.
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Ingår i: International Journal of Legal Medicine. - : Springer Science and Business Media LLC. - 0937-9827 .- 1437-1596. ; 134:1, s. 185-198
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http://dx.doi.org/10... (free)
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- We present results from an inter-laboratory massively parallel sequencing (MPS) study in the framework of the SeqForSTRs project to evaluate forensically relevant parameters, such as performance, concordance, and sensitivity, using a standardized sequencing library including reference material, mixtures, and ancient DNA samples. The standardized library was prepared using the ForenSeq DNA Signature Prep Kit (primer mix A). The library was shared between eight European laboratories located in Austria, France, Germany, The Netherlands, and Sweden to perform MPS on their particular MiSeq FGx sequencers. Despite variation in performance between sequencing runs, all laboratories obtained quality metrics that fell within the manufacturer’s recommended ranges. Furthermore, differences in locus coverage did not inevitably adversely affect heterozygous balance. Inter-laboratory concordance showed 100% concordant genotypes for the included autosomal and Y-STRs, and still, X-STR concordance exceeded 83%. The exclusive reasons for X-STR discordances were drop-outs at DXS10103. Sensitivity experiments demonstrated that correct allele calling varied between sequencing instruments in particular for lower DNA amounts (≤ 125 pg). The analysis of compromised DNA samples showed the drop-out of one sample (FA10013B01A) while for the remaining three degraded DNA samples MPS was able to successfully type ≥ 87% of all aSTRs, ≥ 78% of all Y-STRs, ≥ 68% of all X-STRs, and ≥ 92% of all iSNPs demonstrating that MPS is a promising tool for human identity testing, which in return, has to undergo rigorous in-house validation before it can be implemented into forensic routine casework.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Annan medicin och hälsovetenskap -- Rättsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Other Medical and Health Sciences -- Forensic Science (hsv//eng)
Nyckelord
- Collaborative study
- ForenSeq DNA Signature Prep Kit
- Inter-laboratory study
- Massively parallel sequencing
- Short tandem repeats
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Müller, Petra
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Sell, Christian
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Hadrys, Thorsten
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Hedman, Johannes
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Bredemeyer, Stef ...
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Laurent, Francoi ...
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visa fler...
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Roewer, Lutz
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Achtruth, Sabrin ...
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Sidstedt, Maja
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Sijen, Titia
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Trimborn, Marc
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Weiler, Natalie
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Willuweit, Sasch ...
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Bastisch, Ingo
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Parson, Walther
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