| 1. |
- Ekesbo, Rickard, et al.
(författare)
-
Chronic Helicobacter pylori infection in a population in southern Sweden analysed by histopathology, immunoblot and ELISA serology.
- 2006
-
Ingår i: European Journal of Gastroenterology and Hepathology. - 1473-5687. ; 18:6, s. 589-593
-
Tidskriftsartikel (refereegranskat)abstract
- Background. Many individuals are infected with the bacterium Helicobacter pylori. Some develop ulcers or mucosal atrophy. Aims. To correlate the histological characteristics of the H. pylori-induced gastritis to the immunoblot pattern of the H. pylori infection and to compare the presence of H. pylori bacteria in tissue specimens with ELISA serology and immunoblot analysis. Methods. One hundred and sixty-six consecutive patients were referred to gastroscopy. Forty patients were excluded for various reasons and 126 were included in the study. Results. Twenty-three patients had ulcerations and 25 erosions. Ninety-two (73%) had a chronic gastritis and in 90 (71%) it involved both the antrum and corpus. Ninety-one (72%), of whom 96% had a chronic gastritis, had visible bacteria in the tissue specimens, used as the 'gold standard' for the detection of infection. In patients with chronic gastritis 65 (70%) had positive H. pylori ELISA serology, 27 (30%) had negative H. pylori ELISA, while 76 (83%) had a positive immunoblot pattern. The ELISA positive patients had more advanced chronic gastritis but a lower frequency of metaplasia and atrophy. Acute inflammatory activity in the chronic gastritis had a high immunoreactivity to 120 kDa (CagA) protein and was significantly correlated to antibody reactivity to proteins in the 53-65 kDa range (heat shock proteins) and to a 43 kDa subunit. Metaplasia and atrophy in antrum was associated with a 62 kDa protein band. Conclusion. Almost all H. pylori-infected patients had a pangastritis, visible in both antrum and corpus. Acute inflammatory activity in the chronic gastritis and the presence of metaplasia and atrophy in antrum were associated with a specific immunoblot pattern, indicating infection with more virulent strains. Immunoblot analysis had a better sensitivity than ELISA H. pylori serology.
|
|
| 2. |
- Sjölund, Maria, et al.
(författare)
-
Dissemination of multidrug-resistant bacteria into the Arctic
- 2008
-
Ingår i: Emerging Infectious Diseases. - Atlanta, GA, United States : Centers for Disease Control and Prevention (CDC). - 1080-6040 .- 1080-6059. ; 14:1, s. 70-72
-
Tidskriftsartikel (refereegranskat)abstract
- We show that Escherichia coli isolates originating from Arctic birds carry antimicrobial drug resistance determinants. This finding implies that dissemination of drug-resistant bacteria is worldwide. Resistance genes can be found even in a region where no selection pressure for resistance development exists.
|
|
| 3. |
- Sjölund, Maria, et al.
(författare)
-
Persistence of resistant Staphylococcus epidermidis after single course of clarithromycin
- 2005
-
Ingår i: Emerging Infectious Diseases. - : Centers for Disease Control and Prevention (CDC). - 1080-6040 .- 1080-6059. ; 11:9, s. 1389-1393
-
Tidskriftsartikel (refereegranskat)abstract
- We examined how a common therapy that includes clarithromycin affects normally colonizing Staphylococcus epidermidis. Samples from the nostrils of 5 patients receiving therapy were collected before, immediately after, 1 year after, and 4 years after treatment. From each patient and sample, S. epidermidis strains were isolated and analyzed for clarithromycin susceptibility and presence of the erm(C) gene. We show that macrolide-resistant strains of S. epidermidis were selected during therapy and that the same resistant strain may persist for 4 years, in the absence of further antimicrobial treatment.
|
|
| 4. |
|
|
| 5. |
- Sjölund, Maria, et al.
(författare)
-
Staphylococci in primary skin and soft tissue infections in a Swedish county
- 2008
-
Ingår i: Scandinavian Journal of Infectious Diseases. - : Informa UK Limited. - 0036-5548 .- 1651-1980. ; 40:11-12, s. 894-8
-
Tidskriftsartikel (refereegranskat)abstract
- Patients with skin and soft tissue infections (SSTI) are frequently encountered in primary health care. The majority are uncomplicated and treated empirically by surgical incision and drainage and/or antibiotics. This strategy may risk delaying the detection of methicillin-resistant Staphylococcus aureus (MRSA), which, although still rare in Sweden, is increasingly being found in patients with SSTI. To avoid 'late detection' of MRSA, primary health care physicians in Kronoberg county, Sweden, were asked to perform a culture as soon as the patient's first visit. Samples from 175 patients with primary SSTI confirmed that S. aureus is the dominant pathogen. Two cases of MRSA were detected. Furthermore, isolates of S. aureus producing the Panton-Valentine leukocidin (PVL) toxin were more common among isolates from SSTI than among S. aureus from secondary infections. Finally, we confirmed the importance of the coagulase-negative staphylococcal species S. lugdunensis as a pathogen as it was isolated as the only pathogen in 10% of the skin and soft tissue samples.
|
|
| 6. |
- Sjölund, Maria, 1978-
(författare)
-
What is the great challange?
- 2006
-
Ingår i: Movement as the memory of the body. - Stockholm : University College of Dance. ; , s. 132-133
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)
|
|
| 7. |
- Wierup, Nils, et al.
(författare)
-
Ghrelin and motilin are cosecreted from a prominent endocrine cell population in the small intestine
- 2007
-
Ingår i: Journal of Clinical Endocrinology and Metabolism. - : The Endocrine Society. - 1945-7197 .- 0021-972X. ; 92:9, s. 3573-3581
-
Tidskriftsartikel (refereegranskat)abstract
- Context: Ghrelin is a novel hormone produced mainly in the gastric body. Hitherto, mapping studies of ghrelin cells covering the entire gastrointestinal (GI) tract in humans have been lacking. Furthermore, the phenotype of extragastric ghrelin cells is not known. Objective: The objective of the study was to perform a detailed mapping with specimens from all parts of the GI tract, and colocalization studies to phenotype ghrelin cells along the tract. In addition, mapping of ghrelin cells was performed in porcine GI tract, and the plasma profiles of ghrelin and motilin in blood from the porcine intestine were measured. Design: Biopsies from patients were obtained during gastroscopy or surgery. Ghrelin cell density and phenotyping was assessed with immunocytochemistry, in situ hybridization, and immunogold electron microscopy. Plasma ghrelin and motilin levels were measured in pigs, fitted with cannulas in the mesenteric vein. Results: The upper small intestine is unexpectedly rich in ghrelin cells, and these cells contribute to circulating ghrelin. Ghrelin and motilin are coproduced in the same cells in the duodenum and jejunum of both species, and ghrelin and motilin are stored in all secretory granules of such cells in humans, indicating cosecretion. The plasma profiles of ghrelin and motilin in pig were parallel, and a correlation between ghrelin and motilin ( r(2) = 0.22; P < 0.001) was evident in intestinal blood. Conclusions: The upper small intestine is an important source of ghrelin. The likely cosecretion of intestinal ghrelin and motilin suggests concerted actions of the two hormones. These data may have implications for understanding gut motility and clinical implications for dysmotility and bariatric surgery.
|
|
