Sökning: WFRF:(Sonesson Anders)
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Cytoskeleton compon...
Cytoskeleton components of inside-out and right-side-out plasma membrane vesicles from plants
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- Sonesson, Anders (författare)
- Lund University,Lunds universitet,Medicinska fakultetens centrum för undervisning och lärande, MedCUL,Medicinska fakulteten,Högskolepedagogisk utveckling, AHU,Institutionen för utbildningsvetenskap,Institutioner,Humanistiska och teologiska fakulteterna,The Faculty of Medicine Centre for Teaching and Learning,Faculty of Medicine,Division for Higher Education Development,Department of Educational Sciences,Departments,Joint Faculties of Humanities and Theology
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- Widell, Susanne (författare)
- Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Molecular Cell Biology,Department of Biology,Faculty of Science
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(creator_code:org_t)
- 1993
- 1993
- Engelska 8 s.
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Ingår i: Protoplasma. - 1615-6102. ; 177:1-2, s. 45-52
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Isolated plasma membrane vesicles purified by aqueous polymer two-phase partitioning were used as a model system for studies on the membrane-associated (cortical) cytoskeleton in plants. Actin, as identified by immunoblotting, was found to be specifically attached to plasma membrane vesicles from cauliflower (Brassica oleracea L.). The actin was not washed off as the vesicles were turned inside-out, indicative of a fairly strong attachment. Triton X-100 extraction of plasma membrane vesicles resulted in an insoluble and hence pelletable fraction where actin could be found together with several other proteins. Our results show that the cortical cytoskeleton is to some extent co-purified with the plasma membrane, and we believe that well defined, inside-out and right-side-out plasma membrane vesicles can be used to study the structure and dynamics of the plant cortical cytoskeleton.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Actin
- Cauliflower
- Cortical cytoskeleton
- Membrane cytoskeleton
- Plant plasma membrane.
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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