| 1. |
- Uhlén, Mathias, et al.
(författare)
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A human protein atlas for normal and cancer tissues based on antibody proteomics
- 2005
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Ingår i: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 4:12, s. 1920-1932
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Tidskriftsartikel (refereegranskat)abstract
- Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, similar to 400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.
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| 2. |
- Chen, DeJiu, et al.
(författare)
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Model integration in the development of embedded control systems - A characterization of current research efforts
- 2006
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Ingår i: 2006 IEEE Conference on Computer-Aided Control System Design, Vols 1 and 2. - : IEEE conference proceedings. - 9780780397972 ; , s. 283-289
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Konferensbidrag (refereegranskat)abstract
- The design of advanced embedded control systems requires a systematic approach in handling their increasing complexity and in particular integration of the different aspects and parts of a product worked on by different experts. Several variants of model-based approaches are today advocated to facilitate systems integration. This paper describes a number of representative efforts that address multiple concerns or views including modeling languages such as AADL and EAST-ADL as well as model integration environments such as GeneralStore, ToolNet, and Fujaba. We present a characterization of the approaches and thereby highlight their commonalities and differences regarding basic integration mechanisms and engineering support. We conclude with a prospect for future work.
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| 3. |
- Enarsson, Karin, 1975, et al.
(författare)
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Differential mechanisms for T lymphocyte recruitment in normal and neoplastic human gastric mucosa
- 2006
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Ingår i: Clin Immunol. - : Elsevier BV. ; 118:1, s. 24-34
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Forskningsöversikt (refereegranskat)abstract
- Worldwide, gastric adenocarcinoma (GC) is the second most common cause of death from malignant disease. The reason why immune responses are unable to clear the tumour is not fully understood, although aberrant lymphocyte recruitment to the tumour site might be one factor. Therefore, we investigated the homing phenotype of mucosal T lymphocytes in GC, compared to tumour-free mucosa. We could detect significantly decreased frequencies of mucosal homing alpha4beta7+ T cells in the tumour tissues and increased frequencies of L-selectin+ T cells. This was probably due to the correlated decrease in MAdCAM-1 positive and increase in PNAd positive blood vessels in the tumour mucosa. There were also fewer CXCR3+ T lymphocytes in the tumour tissue. These findings provide evidence that endothelial cells within tumours arising at mucosal sites do not support extravasation of typical mucosa-infiltrating T cells. This may be of major relevance for future immunotherapeutic strategies for treatment of GC.
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| 4. |
- Ericsson, Stefan, et al.
(författare)
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Towards automatic detection of local bearing defects in rotating machines
- 2005
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Ingår i: Mechanical systems and signal processing. - : Elsevier BV. - 0888-3270 .- 1096-1216. ; 19:3, s. 509-535
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Tidskriftsartikel (refereegranskat)abstract
- In this paper we derive and compare several different vibration analysis techniques for automatic detection of local defects in bearings. Based on a signal model and a discussion on to what extent a good bearing monitoring method should trust it, we present several analysis tools for bearing condition monitoring and conclude that wavelets are especially well suited for this task. Then we describe a large-scale evaluation of several different automatic bearing monitoring methods using 103 laboratory and industrial environment test signals for which the true condition of the bearing is known from visual inspection. We describe the four best performing methods in detail (two wavelet-based, and two based on envelope and periodisation techniques). In our basic implementation, without using historical data or adapting the methods to (roughly) known machine or signal parameters, the four best methods had 9-13% error rate and are all good candidates for further fine-tuning and optimisation. Especially for the wavelet-based methods, there are several potentially performance improving additions, which we finally summarise into a guiding list of suggestion.
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| 5. |
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| 6. |
- Gustafsson, Erik, 1976-
(författare)
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Optimization of Castings by using Surrogate Models
- 2007
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- In this thesis structural optimization of castings and thermomechanical analysis of castings are studied.In paper I an optimization algorithm is created by using Matlab. The algorithm is linked to the commercial FE solver Abaqus by using Python script. The optimization algorithm uses the successive response surfaces methodology (SRSM) to create global response surfaces. It is shown that including residual stresses in structural optimization of castings yields an optimal shape that differs significantly from the one obtained when residual stresses are excluded.In paper II the optimization algorithm is expanded to using neural networks. It is tested on some typical bench mark problems and shows very promising results. Combining paper I and II the response surfaces can be either analytical functions, both linear and non-linear, or neural networks. The optimization is then performed by using sequential linear programming or by using a zero-order method called Complex. This is all gathered in a package called StuG-OPT.In paper III and IV focus is on the thermomechanical problem when residual stresses are calculated. In paper III a literature review is performed and some numerical simulations are performed to see where numerical simulations can be used in the industry today. In paper IV simulations are compared to real tests. Several stress lattices are casted and the residual stresses are measured. Simulations are performed by using Magmasoft and Abaqus. In Magmasoft a J2-plasticity model is used and in Abaqus two simulations are performed using either J2-plasticity or the ”Cast Iron Plasticity” available in Abaqus that takes into account the different behavior in tension and compression for grey cast iron.
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| 7. |
- Gustafsson, Erik, et al.
(författare)
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Residual stresses in a stress lattice : experiments and finite element simulations
- 2009
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Ingår i: Journal of Materials Processing Technology. - : Elsevier. - 0924-0136 .- 1873-4774. ; 209:9, s. 4320-4328
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Tidskriftsartikel (refereegranskat)abstract
- In this work residual stresses in a stress lattice are studied. The residual stresses are both measured and simulated. The stress lattice is casted of low alloyed grey cast iron. In fact, nine similar lattices are casted and measured. The geometry of the lattice consists of three sections in parallel. The diameter of the two outer sections are thinner than the section in the middle. When the stress lattice cools down, this difference in geometry yields that the outer sections start to solidify and contract before the section in the middle. Finally, an equilibrium state, with tensile stresses in the middle and compressive stresses in the outer sections, is reached. The thermo-mechanical simulation of the experiments is performed by using Abaqus. The thermo-mechanical solidification is assumed to be uncoupled. First a thermal analysis, where the lattice is cooled down to room temperature, is performed. Latent heat is included in the analysis by letting the fraction of solid be a linear function of the temperature in the mushy zone. After the thermal analysis a quasi-static mechanical analysis is performed where the temperature history is considered to be the external force. A rate independent J2-plasticity model with isotropic hardening is considered, where the material data depend on the temperature. Tensile tests are performed at room temperature, 200°C, 400°C, 600°C and 800°C in order to evaluate the Young´s modulus, the yield strength and the hardening accurate. In addition, the thermal expansion coefficient is evaluated for temperatures between room temperature and 1000°C. The state of residual stresses is measured by cutting the mid section or the outer section. The corresponding elastic spring-back reveals the state of residual stresses. The measured stresses are compared to the numerical simulations. The simulations show good agreement with the results from the experiments.
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| 8. |
- Gustafsson, Erik, et al.
(författare)
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Shape Optimization of Castings by using Successive Response Surface Methodology
- 2008
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Ingår i: Structural and multidisciplinary optimization (Print). - : Springer Science and Business Media LLC. - 1615-147X .- 1615-1488. ; 35:1, s. 11-28
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Tidskriftsartikel (refereegranskat)abstract
- In this paper, an optimization routine for a thermomechanical problem is presented. The optimization routine is based on the successive response surface methodology where the panning and zooming technique presented by Stander and Craig has been implemented and improved. The optimization routine has been applied to an optimization problem of a three-dimensional beam that undergoes a solidification process. The material in the beam is assumed to be low-alloyed gray iron. The thermomechanical solidification analysis is uncoupled where, first, a thermal analysis is performed to determine the thermal history. This thermal history is then used to calculate the residual stresses in the beam. The residual stresses are solved by using classical J 2-plasticity with temperature-dependent material properties. The residual stresses from solidification are then carried on to the structural analysis where a mechanical load is applied. These are all linked together via scripts, and the commercial FE software Abaqus is used as the FE solver. The obtained maximum von Mises stress and mass information for every set of parameters are then exported to Matlab where general quadratic response surfaces are fitted by a least square method. Taken together, these response surfaces define a minimum of weight problem, which is solved by using sequential linear programming. To minimize the number of evaluations needed, the parameters are chosen to be D-optimally selected. The numerical results show that the residual stresses from solidification might influence the optimal shape significantly. The residual stress results have been compared with those obtained from casting simulation softwares, and the results are similar. The optimization has been compared with a commercial optimization software and shows very promising results.
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| 9. |
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| 10. |
- Gustafsson, Erik, et al.
(författare)
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Structural optimization of castings by using ABAQUS and Matlab
- 2005
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Ingår i: Competent Design by Castings. - Espoo, Finland : VTT. - 9513862984 - 9513862992 ; , s. 39-52
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Konferensbidrag (refereegranskat)abstract
- In this work a general method for structural optimization of nonlinear structures is implemented using FE-analysis. The method utilizes the response surface methodology with polynomial surfaces and nonlinear programming. In such manner a method that is applicable for a large number of different classes of nonlinear problems is obtained. In this paper, the method is utilized to minimize weight of castings by including residual stresses from solidification. This is performed by first determine the residual stresses by a thermomechanical analysis of a metal structure that is cooled from a temperature above liquidus temperature down to room temperature. The thermomechanical analysis is uncoupled where the temperature distribution within the casting as a function of time is determined first and is later on used for residual stress calculations. These residual stresses are then included when the mechanical load is applied to the structure and the problem of minimum of weight is formulated. The structure shown in this paper is an example of a two dimensional geometry. The shape of the structures will of course affect the residual stress distribution during the optimization. The nonlinear models are then solved using ABAQUS/Standard. A set of solutions are generated by solving the model for a pre-defined set of parameters. In order to minimize the number of simulations and still achieve good surface approximations these parameters are taken to be D-optimal. The sets of solutions and parameters are in turn exported to Matlab where general quadratic response surfaces are fitted by the least square method. By utilizing these surfaces the problem of minimum of weight subjected to constraints on stresses is formulated. Finally, the nonlinear optimization problem is solved by sequential linear programming.
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