| 1. |
- Jansson, Christer, et al.
(författare)
-
Cassava, a potential biofuel crop in (the) People's Republic of China
- 2009
-
Ingår i: Applied Energy. - : Elsevier BV. - 0306-2619 .- 1872-9118. ; 86, s. S95-S99
-
Tidskriftsartikel (refereegranskat)abstract
- Cassava ranks fifth among crops in global starch production. It is used as staple food in many tropical countries of Africa, Asia and Latin America. In (the) People's Republic of China, although not yet a staple food, cassava is of major economic importance for starch for a large area of southern (the) PRC, especially in the provinces of Guangdong, Guanxi, Yunnan and Hainan. Recently, cassava-derived bioethanol production has been increasing due to its economic benefits compared to other bioethanol-producing crops in the country. We discuss here the possible potentials of cassava for bioethanol production
|
|
| 2. |
|
|
| 3. |
- Mutisya, Joel, et al.
(författare)
-
Diurnal oscillation of SBE expression in sorghum endosperm
- 2009
-
Ingår i: Journal of plant physiology (Print). - : Elsevier BV. - 0176-1617 .- 1618-1328. ; 166:4, s. 428-434
-
Tidskriftsartikel (refereegranskat)abstract
- Summary Spatial and temporal expression patterns of the sorghum SBEI, SBEIIA and SBEIIB genes, encoding, respectively, starch branching enzyme (SBE) I, IIA and IIB, in the developing endosperm of sorghum (Sorghum bicolor) were studied. Full-length genomic and cDNA clones for sorghum were cloned, and the SBEIIA cDNA was used together with gene-specific probes for sorghum SBEIIB and SBEI. In contrast to sorghum SBEIIB, which was expressed primarily in endosperm and embryo, SBEIIA was also expressed in vegetative tissues. All three genes shared a similar temporal expression profile during endosperm development, with a maximum activity at 15–24 d after pollination. This differed from barley and maize, in which SBEI gene activity showed a significantly later onset compared to that of SBEIIA and SBEIIB. Expression of the three SBE genes in the sorghum endosperm exhibited a diurnal rhythm during a 24-h cycle.
|
|
| 4. |
- Mutisya, Joel, et al.
(författare)
-
Transcriptional regulation of the sbeIIb genes in sorghum (Sorghum bicolor) and barley (Hordeum vulgare) : Importance of the barley sbeIIb second intron
- 2006
-
Ingår i: Journal of plant physiology (Print). - : Elsevier BV. - 0176-1617 .- 1618-1328. ; 163:7, s. 770-780
-
Tidskriftsartikel (refereegranskat)abstract
- Summary The transcriptional activity of the sorghum sbeIIb gene, encoding starch branching enzyme IIb, is seed specific, with expression in both the endosperm and the embryo. In comparison, expression of barley sbeIIb is confined to the endosperm, whereas that of barley sbeIIa occurs in endosperm, embryonic and vegetative tissues. It has been suggested that the second intron of barley sbeIIb may be instrumental in conferring endosperm-specific expression. Therefore, to further investigate the regulatory mechanisms of barley and sorghum sbe, we examined the tissue-specific activity of the sorghum sbe promoter in transient assays of green fluorescent protein (gfp) reporter constructs. We found that, when linked to the barley sbeIIb second intron, the sorghum sbeIIb promoter could not drive gfp transcription in sorghum or barley embryonic cells. Similar results were obtained for the barley sbeIIa promoter. Database searches showed that sequences homologous to the barley sbeIIb intron also exist in introns and flanking regions of some other grass genes. Deletion mutagenesis of the sorghum sbeIIb promoter identified the minimal promoters required for high- and low-level expression, respectively, but did not reveal any putative promoter elements crucial for expression. A sequence with similarity to the SURE element, implicated in sugar signaling, was located in the distal promoter region of sorghum sbeIIb, upstream of the minimal promoters. SURE elements are present in the proximal promoter regions of the sugar-regulated barley iso1 gene, and barley sbeIIb. In keeping in line with these observations, RNA-gel blot analyses demonstrated that expression of barley sbeIIb was sugar inducible, whereas that of sorghum sbeIIb was not.
|
|
