| 1. |
- Andersson, Annica, et al.
(författare)
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Relationship of grain fructan content to degree of polymerisation in different barleys
- 2014
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Ingår i: Food and Nutrition Sciences. - : Scientific Research Publishing, Inc.. - 2157-944X .- 2157-9458. ; 5, s. 581-589
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Tidskriftsartikel (refereegranskat)abstract
- Fructans are important in the survival of plants and also valuable for humans as potentially health promoting food ingredients. In this study fructan content and composition were determined in grains of 20 barley breeding lines and cultivars with a wide variation in chemical composition, morphology and country of origin, grown at one site in Chile. There was significant genotypic variation in grain fructan content ranging from 0.9% to 4.2% of grain dry weight. Fructan degree of polymerisation (DP) was analysed using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Changes in the distribution of different chain lengths and the pattern of structures of fructan were detected with increasing amount of fructan in the different barleys. A positive correlation was found between fructan content and the relative amount of long chain fructan (DP > 9) (r = 0.54, p = 0.021). Our results provide a basis for selecting promising barley lines and cultivars for further research on fructan in barley breeding with the aim to produce healthy food products.
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| 2. |
- Aslan, Selcuk, et al.
(författare)
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Wax esters of different compositions produced via engineering of leaf chloroplast metabolism in Nicotiana benthamiana
- 2014
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Ingår i: Metabolic Engineering. - : Elsevier BV. - 1096-7176 .- 1096-7184. ; 25, s. 103-112
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Tidskriftsartikel (refereegranskat)abstract
- In a future bin based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de nova fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl recluctases (EAR) and wax ester synthases (WS) were transiently expressed in Nic"onana benthamiuna loaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. (C) 2014 The Authors. Published by Elsevier Inc. On behalf of International Metabolic Engineering Society.
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| 3. |
- Nalawade, Satish, et al.
(författare)
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Development of an efficient tissue culture after crossing (TCC) system for transgenic improvement of barley as a bioenergy crop
- 2012
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Ingår i: Applied Energy. - : Elsevier BV. - 0306-2619 .- 1872-9118. ; 91, s. 405-411
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Tidskriftsartikel (refereegranskat)abstract
- We have developed a protocol for rapid generation of barley plants with introduced genes by employing techniques of tissue culture, plant regeneration and crossing. Compared to conventional protocols, the time between pollination of To plants and identification of phenotypic expression in the T(1) generation is reduced from around 17-9 weeks. All of the selected candidates after the tissue culture screening exhibited phenotypic expression. Furthermore, by utilizing ordinary pollen crossing, we demonstrated a route for introduction of genes to a recalcitrant barley elite cultivar. This Tissue Culture after Crossing (TCC) procedure significantly enhances the prospects for producing transgenic barley lines from desirable germplasm. We suggest that the TCC method will contribute to improve barley as a promising bioenergy crop. (C) 2011 Elsevier Ltd. All rights reserved.
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| 4. |
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| 5. |
- Sun, Chuanxin
(författare)
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The Future Crops for Biofuels
- 2011
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Ingår i: Economic Effects of Biofuel Production. - : InTech. - 9789533071787 ; , s. 25-38
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 6. |
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| 7. |
- Xie, Zhoupeng, et al.
(författare)
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A selection strategy in plant transformation based on antisense oligodeoxynucleotide inhibition
- 2014
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Ingår i: Plant Journal. - : Wiley. - 0960-7412 .- 1365-313X. ; 77, s. 954-961
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Tidskriftsartikel (refereegranskat)abstract
- Antisense oligodeoxynucleotide (asODN) inhibition was developed in the 1970s, and since then has been widely used in animal research. However, in plant biology, the method has had limited application because plant cell walls significantly block efficient uptake of asODN to plant cells. Recently, we have found that asODN uptake is enhanced in a sugar solution. The method has promise for many applications, such as a rapid alternative to time-consuming transgenic studies, and high potential for studying gene functionality in intact plants and multiple plant species, with particular advantages in evaluating the roles of multiple gene family members. Generation of transgenic plants relies on the ability to select transformed cells. This screening process is based on co-introduction of marker genes into the plant cell together with a gene of interest. Currently, the most common marker genes are those that confer antibiotic or herbicide resistance. The possibility that traits introduced by selectable marker genes in transgenic field crops may be transferred horizontally is of major public concern. Marker genes that increase use of antibiotics and herbicides may increase development of antibiotic-resistant bacterial strains or contribute to weed resistance. Here, we describe a method for selection of transformed plant cells based on asODN inhibition. The method enables selective and high-throughput screening for transformed cells without conferring new traits or functions to the transgenic plants. Due to their high binding specificity, asODNs may also find applications as plant-specific DNA herbicides.
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