| 1. |
- Klionsky, Daniel J., et al.
(författare)
-
Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
-
Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
-
Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
|
|
| 2. |
- Deng, Min, et al.
(författare)
-
Genome-wide association analyses in Han Chinese identify two new susceptibility loci for amyotrophic lateral sclerosis
- 2013
-
Ingår i: Nature Genetics. - : Nature Publishing Group. - 1061-4036 .- 1546-1718. ; 45:6, s. 697-
-
Tidskriftsartikel (refereegranskat)abstract
- To identify susceptibility genes for amyotrophic lateral sclerosis (ALS), we conducted a genome-wide association study (GWAS) in 506 individuals with sporadic ALS and 1,859 controls of Han Chinese ancestry. Ninety top SNPs suggested by the current GWAS and 6 SNPs identified by previous GWAS were analyzed in an independent cohort of 706 individuals with ALS and 1,777 controls of Han Chinese ancestry. We discovered two new susceptibility loci for ALS at 1q32 (CAMK1G, rs6703183, P-combined = 2.92 x 10(-8), odds ratio (OR) = 1.31) and 22p11 (CABIN1 and SUSD2, rs8141797, P-combined = 2.35 x 10(-9), OR = 1.52). These two loci explain 12.48% of the overall variance in disease risk in the Han Chinese population. We found no association evidence for the previously reported loci in the Han Chinese population, suggesting genetic heterogeneity of disease susceptibility for ALS between ancestry groups. Our study identifies two new susceptibility loci and suggests new pathogenic mechanisms of ALS.
|
|
| 3. |
- Huang, Hongyun, et al.
(författare)
-
Consensus of Clinical Neurorestorative Progress in Patients With Complete Chronic Spinal Cord Injury
- 2014
-
Ingår i: Cell Transplantation. - 0963-6897 .- 1555-3892. ; 23:S1, s. S5-S17
-
Forskningsöversikt (refereegranskat)abstract
- Currently, there is a lack of effective therapeutic methods to restore neurological function for chronic complete spinal cord injury (SCI) by conventional treatment. Neurorestorative strategies with positive preclinical results have been translated to the clinic, and some patients have gotten benefits and their quality of life has improved. These strategies include cell therapy, neurostimulation or neuromodulation, neuroprosthesis, neurotization or nerve bridging, and neurorehabilitation. The aim of this consensus by 31 experts from 20 countries is to show the objective evidence of clinical neurorestoration for chronic complete SCI by the mentioned neurorestorative strategies. Complete chronic SCI patients are no longer told, "nothing can be done." The clinical translation of more effective preclinical neurorestorative strategies should be encouraged as fast as possible in order to benefit patients with incurable CNS diseases. This manuscript is published as part of the International Association of Neurorestoratology (IANR) special issue of Cell Transplantation.
|
|
| 4. |
- Weinstein, John N., et al.
(författare)
-
The cancer genome atlas pan-cancer analysis project
- 2013
-
Ingår i: Nature Genetics. - : Springer Science and Business Media LLC. - 1061-4036 .- 1546-1718. ; 45:10, s. 1113-1120
-
Forskningsöversikt (refereegranskat)abstract
- The Cancer Genome Atlas (TCGA) Research Network has profiled and analyzed large numbers of human tumors to discover molecular aberrations at the DNA, RNA, protein and epigenetic levels. The resulting rich data provide a major opportunity to develop an integrated picture of commonalities, differences and emergent themes across tumor lineages. The Pan-Cancer initiative compares the first 12 tumor types profiled by TCGA. Analysis of the molecular aberrations and their functional roles across tumor types will teach us how to extend therapies effective in one cancer type to others with a similar genomic profile. © 2013 Nature America, Inc. All rights reserved.
|
|
| 5. |
- Chen, Bin, et al.
(författare)
-
TICT based fluorescence "turn-on" hydrazine probes
- 2014
-
Ingår i: Sensors and actuators. B, Chemical. - : Elsevier BV. - 0925-4005 .- 1873-3077. ; 199, s. 93-100
-
Tidskriftsartikel (refereegranskat)abstract
- Fluorescence "turn-on" probes PC3 and C3 with weak background emissions were developed for hydrazine sensing. The aldehyde and dicyanovinyl groups were used as the recognition units for PD and C3, respectively. Because of low reactivity of the aldehyde group, the fluorescence of PC3 was enhanced by only ca. 93 folds upon addition of a large amount of 1646 eq. hydrazine. In contrast, 0 exhibited fluorescence enhancement by ca. 239 folds upon addition of only 1.3 eq. hydrazine, and thus it showed high sensitivity towards hydrazine, with the detection limit of 7 ppb. In aqueous systems, it also works well with improved selectivity for hydrazine over CN-. The weak fluorescence of PC3 and 0 can be ascribed to twisted intramolecular charge transfer (TICT) processes by the combination of the bulky diphenylamino and 9-anthryl units, which were well demonstrated by theoretical calculations, viscosity dependent fluorescence, and fluorescence decay behaviour. Addition of hydrazine induced the disappearance of the TICT deactivation pathway, resulting in the observed fluorescence enhancement. It can be concluded that the combination of the bulky diphenylamino and 9-anthryl units is an effective approach for developing fluorescence turn-on hydrazine probes based on the TICT mechanism.
|
|
| 6. |
- Sun, Qiming, et al.
(författare)
-
High performance nanosheet-like silicoaluminophosphate molecular sieves : synthesis, 3D EDT structural analysis and MTO catalytic studies
- 2014
-
Ingår i: Journal of Materials Chemistry A. - : Royal Society of Chemistry (RSC). - 2050-7488. ; 2:42, s. 17828-17839
-
Tidskriftsartikel (refereegranskat)abstract
- Nanosheet-like silicoaluminophosphate (SAPO) molecular sieves SAPO-34 (CHA-type) and SAPO-18 (AEI-type) with different silicon contents were synthesized under hydrothermal conditions by using tetraethylammonium hydroxide as the template. Three-dimensional electron diffraction tomography (3D EDT) technique was applied for ab initio structure solutions. Electron microscopy observations confirmed the existence of defects, i.e., intergrowth of CHA- and AEI-type frameworks caused by the different stacking manners of double 6-ring layers, but the layers were highly coherent along c direction. Compositions, acidities, chemical environments, and texture properties of all the samples were characterized by ICP, EDS, NH3-TPD, MAS NMR, and N-2 adsorption-desorption measurements. The catalytic performances of methanol-to-olefin (MTO) reactions over nano SAPO catalysts with different silicon contents were systematically studied. All of these catalysts showed excellent catalytic activity, among which SAPO-34 showed superior catalytic performance compared to SAPO-18. DFT calculations were utilized to study the different catalytic performance of CHA and AEI. Significantly to date, SAPO-34 with the lowest silicon content exhibited the longest catalyst lifetime and the lowest coking rate in the MTO reaction than any of the reported catalysts, tested under similar conditions. The straight 8-ring pore channel along the c direction provided the optimum diffusion pathway as well as the shortest diffusion length for reactant and generated olefins, thus significantly reduced the coking rate. This work demonstrates that a 3D EDT approach combined with TEM and EDS analysis from a single nanocrystal can provide a clear crystal structure, crystal orientation and compositional information of nanocrystals, which are useful for the better understanding of the catalytic performance of nanosized crystalline catalysts.
|
|
| 7. |
|
|
| 8. |
- Yang, Tao, et al.
(författare)
-
Regional frequency analysis and spatio-temporal pattern characterization of rainfall extremes in the Pearl River Basin, China
- 2010
-
Ingår i: Journal of Hydrology. - : Elsevier BV. - 0022-1694 .- 1879-2707. ; 380:3-4, s. 386-405
-
Tidskriftsartikel (refereegranskat)abstract
- This paper presents a method for regional frequency analysis and spatio-temporal pattern characterization of rainfall-extreme regimes (i.e. extremes, durations and timings) in the Pearl River Basin (PRB) using the well-known L-moments approach together with advanced statistical tests including stationarity test and serial correlation check, which are crucial to the valid use of L-moments for frequency analysis. Results indicate that: (1) the entire Pearl River Basin (40 sites) can be categorized into six regions by cluster analysis together with consideration of the topography and spatial patterns of mean precipitation in the basin. The results of goodness-of-fit measures indicate that the GNO, GLO, GEV, and PE3 distributions fit well for most of the basin for different HOM regions, but their performances are slightly different in term of curve fitting; (2) the estimated quantiles and their biases approximated by Monte Carlo simulation demonstrate that the results are reliable enough for the return periods of less than 100 years; (3) excessive precipitation magnitude records are observed at Guilin region of Guangxi Province and Fogang region of Guangdong Province, which have sufficient climate conditions (e.g. precipitation and humidity) responsible for the frequently occurred flood disasters in the regions. In addition, the spatial variations of precipitation in different return periods (Return period = 1, 10, 50 years to 100 years) increase from the upstream to downstream at the regional scale; (4) the seasonal patterns of precipitation extremes for different topographical regions are different. The major precipitation events of AM1R, AM3R, AM5R and AM7R in regions of low-elevation in lower (south-eastern) part of the basin occur mainly in May, June, July and August, while the main precipitation periods for the mountainous region upstream are June, July and August. Further analysis of the NCAR/NCEP reanalysis data indicates that the eastern Asian summer monsoon and typhoons (or hurricanes) are major metrological driving forces on the precipitation regimes. Additionally, topographical features (i.e. elevation, distance to the sea, and mountain’s influences) also exert different impacts on the spatial patterns of such regimes. To the best of our knowledge, this study is the first attempt to conduct a systematic regional frequency analysis on various annual precipitation extremes (based on consecutive 1-, 3-, 5-, 7-day averages) and to establish the possible links to climate pattern and topographical features in the Pearl River Basin and even in China. These findings are expected to contribute to exploring the complex spatio-temporal patterns of extreme rainfall in this basin in order to reveal the underlying linkages between precipitation and floods from a broad geographical perspective. (C) 2009 Published by Elsevier B.V.
|
|
| 9. |
- Zhang, Wei-Min, et al.
(författare)
-
Synthesis of mesoporous silica by a surface charge reversal route
- 2010
-
Ingår i: Journal of Colloid and Interface Science. - : Elsevier BV. - 0021-9797 .- 1095-7103. ; 349:2, s. 473-476
-
Tidskriftsartikel (refereegranskat)abstract
- Pore size adjustable mesoporous silica was synthesized by adsorption of varying amount of sodium dodecyl benzenesulfonate at the surface of silica activated by zinc ion via a novel surface charge reversal route. The pore size and volume can be adjusted from 5.9 to 13.76 nm and 0.88 to 1.08 cm3·g-1 respectively with increasing the SDBS concentration from 0.77 to 3.08 mmol·L-1. Adsorption of Zn2+ as a function of pH and N2 adsorption/desorption isotherms demonstrated that the metal ions such as Zn2+ could be readily removed with dilute nitric acid without apparent collapse of the pore structure at the proper range of SDBS concentration.
|
|
| 10. |
- Zhang, Wei-Min, et al.
(författare)
-
Synthesis of mesoporous ZnS synergistically templated by butylamine and alkanols
- 2012
-
Ingår i: Microporous and Mesoporous Materials. - : Elsevier BV. - 1387-1811 .- 1873-3093. ; 147:1, s. 222-228
-
Tidskriftsartikel (refereegranskat)abstract
- Pore size and pore volume adjustable mesoporous ZnS was synthesized through a co-template method, which was achieved by the combined interaction between butylamine and some alkanols with proper lengths of the straight carbon chain. The pore size for mesoporous ZnS templated by butyl amine alone was 4.29 nm, and could be enlarged to 6.96 and 8.33 nm respectively through adding certain amounts of hexanol and octanol. Correspondingly, the pore volume also exhibited an augmentation with increasing carbon chain lengths of alkanols from C6 to C8. However, the pore size and pore volume dropped abruptly when decanol was added as the auxiliary agent. The formation of mesopores for ZnS prepared using butylamine molecules as the only templating agent is considered to be attributed to the coordination between N atoms in amines and Zn2+ ions at the surfaces of zinc suphide. The templating effect of butylamines might be improved by adding hexanols and octanols to form aggregates through solubilization to tailor the pore size and pore volume of ZnS effectively, while the function of decanols for changing the porous structure is restricted by its low solubility.
|
|
