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- 2019
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Tidskriftsartikel (refereegranskat)
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| 3. |
- Zhang, Yuehui, et al.
(författare)
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Hyperandrogenism and insulin resistance contribute to hepatic steatosis and inflammation in female rat liver.
- 2018
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Ingår i: Oncotarget. - : Impact Journals, LLC. - 1949-2553. ; 9:26, s. 18180-18197
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Tidskriftsartikel (refereegranskat)abstract
- Women with polycystic ovary syndrome (PCOS) are at high risk for nonalcoholic fatty liver disease (NAFLD). While insulin resistance is a common trait for both PCOS and NAFLD, hyperandrogenism is also considered to be a key factor contributing to PCOS, and the molecular mechanisms behind the interactions between insulin resistance and hyperandrogenism in the female liver remain largely unexplored. Using chronic treatment with insulin and/or human chorionic gonadotropin (hCG), we showed that all female rats with different treatments induced imbalance between de novo lipogenesis and mitochondrial β-oxidation via the Pparα/β-Srebp1/2-Acc1 axis, resulting in varying degrees of hepatic steatosis. Given the fact that hepatic lipid metabolism and inflammation are tightly linked processes, we found that hCG-induced hyperandrogenic rats had strongly aggravated hepatic inflammation. Further mechanistic investigations revealed that dysregulation of the IRS-PI3K-Akt signaling axis that integrated aberrant inflammatory, apoptotic and autophagic responses in the liver was strongly associated with hyperandrogenism itself or combined with insulin resistance. Additionally, we found that hCG-treated and insulin+hCG-induced rats developed visceral adipose tissue inflammation characterized by the presence of "crown like" structure and increased inflammatory gene expression. Because a more pronounced hepatic steatosis, inflammatory responses, and hepatocyte cell damage were observed in insulin+hCG-induced PCOS-like rats, our finding suggest that NAFLD seen in PCOS patients is dependent of hyperandrogenism and insulin resistance.
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- Zhang, Yuehui, et al.
(författare)
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Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus.
- 2016
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Ingår i: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 6
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Tidskriftsartikel (refereegranskat)abstract
- Peripheral insulin resistance and hyperandrogenism are the primary features of polycystic ovary syndrome (PCOS). However, how insulin resistance and hyperandrogenism affect uterine function and contribute to the pathogenesis of PCOS are open questions. We treated rats with insulin alone or in combination with human chorionic gonadotropin (hCG) and showed that peripheral insulin resistance and hyperandrogenism alter uterine morphology, cell phenotype, and cell function, especially in glandular epithelial cells. These defects are associated with an aberration in the PI3K/Akt signaling pathway that is used as an indicator for the onset of insulin resistance in classical metabolic tissues. Concomitantly, increased GSK3β (Ser-9) phosphorylation and decreased ERK1/2 phosphorylation in rats treated with insulin and hCG were also observed. We also profiled the expression of glucose transporter (Glut) isoform genes in the uterus under conditions of insulin resistance and/or hyperandrogenism. Finally, we determined the expression pattern of glycolytic enzymes and intermediates during insulin resistance and hyperandrogenism in the uterus. These findings suggest that the PI3K/Akt and MAPK/ERK signaling pathways play a role in the onset of uterine insulin resistance, and they also suggest that changes in specific Glut isoform expression and alterations to glycolytic metabolism contribute to the endometrial dysfunction observed in PCOS patients.
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- Sun, Xiaoyan, et al.
(författare)
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APOE ε4 carriers may undergo synaptic damage conferring risk ofAlzheimer's disease.
- 2016
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Ingår i: Alzheimer's & dementia : the journal of the Alzheimer's Association. - : Wiley. - 1552-5279. ; 12:11, s. 1159-1166
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Tidskriftsartikel (refereegranskat)abstract
- Pathogenesis of Alzheimer's disease (AD) in apolipoprotein E ε4 (APOE ε4) carriers remains unclear. We hypothesize that APOE isoforms have differential effects on synaptic function.
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| 7. |
- Sun, Yunhao, et al.
(författare)
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Modeling Thermodynamic Derivative Properties and Gas Solubility of Ionic Liquids with ePC-SAFT
- 2019
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Ingår i: Industrial & Engineering Chemistry Research. - : American Chemical Society (ACS). - 0888-5885 .- 1520-5045. ; 58:19, s. 8401-8417
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Tidskriftsartikel (refereegranskat)abstract
- In this work, the ion-specific electrolyte perturbed-chain statistical associating fluid theory (ePC-SAFT) was extended to predict the second-order thermodynamic derivative properties and gas solubility of the ionic liquids (ILs) containing one of the IL cations ([Cnmim]+, [Cnpy]+, [Cnmpy]+, [Cnmpyr]+, and [THTDP]+) and one of the IL anions ([Tf2N]−, [PF6]−, [BF4]−, [tfo]−, [DCA]−, [SCN]−, [C1SO4]−, [C2SO4]−, [eFAP]−, Cl–, [Ac]−, and Br–). The ideal-gas isobaric heat capacities of ILs were estimated by the group contribution method for obtaining the heat capacity. The model prediction results were compared with the available experimental data, and the comparison shows that the ePC-SAFT prediction is reliable for most ILs. Furthermore, one adjustable ion-specific binary interaction parameter between the IL ion and CO2 can be used to further improve the model prediction performance for the CO2 solubility in ILs.
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- Sun, Yunhao, et al.
(författare)
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Modeling Viscosity of Ionic Liquids with Electrolyte Perturbed-Chain Statistical Associating Fluid Theory and Free Volume Theory
- 2018
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Ingår i: Industrial & Engineering Chemistry Research. - : American Chemical Society (ACS). - 0888-5885 .- 1520-5045. ; 57:26, s. 8784-8801
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Tidskriftsartikel (refereegranskat)abstract
- Viscosity is one of the most important physical properties when developing ionic liquids (ILs) for industrial applications such as CO2 separation. The viscosities of ILs have been measured experimentally, while the modeling work is still limited. In this work, the electrolyte perturbed-chain statistical associating fluid theory (ePC-SAFT) was combined with the free volume theory (FVT) to model the viscosities of pure ILs and IL mixtures up to high pressures and temperatures, in which the ePC-SAFT was used to calculate the density as inputs for modeling the viscosity of ILs with FVT. The ILs under consideration contain one of the IL cations [Cnmim]+, [Cnpy]+, [Cnmpy]+, [Cnmpyr]+, or [THTDP]+ and one of the IL anions [Tf2N]−, [PF6]−, [BF4]−, [tfo]−, [DCA]−, [SCN]−, [C1SO4]−, [C2SO4]−, [eFAP]−, Cl–, [Ac]−, or Br–. In total, 89 ILs were considered combined with a thorough literature survey of the available experimental viscosity data and evaluation. The comparison with the available experimental viscosities shows that the model can provide reliable representation and prediction for most of the pure ILs in a wide temperature and pressure range, and it can be further used to predict and describe the viscosity of IL mixtures reliably.
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- Wu, Di, et al.
(författare)
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Profiling surface proteins on individual exosomes using a proximity barcoding assay
- 2019
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Ingår i: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- Exosomes have been implicated in numerous biological processes, and they may serve as important disease markers. Surface proteins on exosomes carry information about their tissues of origin. Because of the heterogeneity of exosomes it is desirable to investigate them individually, but this has so far remained impractical. Here, we demonstrate a proximity-dependent barcoding assay to profile surface proteins of individual exosomes using antibody-DNA conjugates and next-generation sequencing. We first validate the method using artificial streptavidin-oligonucleotide complexes, followed by analysis of the variable composition of surface proteins on individual exosomes, derived from human body fluids or cell culture media. Exosomes from different sources are characterized by the presence of specific combinations of surface proteins and their abundance, allowing exosomes to be separately quantified in mixed samples to serve as markers for tissue-specific engagement in disease.
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- Yuan, Shengjuan, et al.
(författare)
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CO2 absorption in mixed aqueous solution of MDEA and cholinium glycinate
- 2017
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Ingår i: Energy & Fuels. - : American Chemical Society (ACS). - 0887-0624 .- 1520-5029. ; 31:7, s. 7325-7333
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Tidskriftsartikel (refereegranskat)abstract
- A new mixed solvent system that consists of cholinium glycinate ([Cho][Gly]) and aqueous N-methyldiethanolamine (MDEA) solution was developed in this work to serve as CO2 absorbent. The equilibrium absorption was carried out to investigate the effect of solution composition, pressure and temperature on CO2 absorption performance. The effect of CO2 absorption on the viscosity of the aqueous solutions was studied, and the regeneration efficiency of the aqueous solutions was also investigated. The results showed that the CO2 absorption loading decreased with increasing [Cho][Gly] concentration and temperature, and the absorption loading strongly depended on CO2 partial pressure. The reactivity of MDEA was significantly enhanced with the addition of [Cho][Gly]. The aqueous solution with (10 wt % [Cho][Gly] + 20 wt % MDEA) showed an optimal CO2 absorption and high regeneration efficiency. Furthermore, the CO2 absorption mechanism in the aqueous [Cho][Gly]-MDEA solution was explored by 13C Nuclear Magnetic Resonance (NMR), which indicated that the CO2 absorption in the aqueous [Cho][Gly]-MDEA solution was zwitterion mechanism.
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