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Träfflista för sökning "WFRF:(Sutherland Duncan) srt2:(2005-2009)"

Sökning: WFRF:(Sutherland Duncan) > (2005-2009)

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  • Agheli, Hossein, 1965, et al. (författare)
  • Nanostructured biointerfaces
  • 2006
  • Ingår i: Materials Science and Engineering C. ; 26, s. 911-917
  • Tidskriftsartikel (refereegranskat)
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  • Dahlin, Andreas, 1980, et al. (författare)
  • Localized surface plasmon sensing of lipid-membrane-mediated biorecognition events
  • 2005
  • Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 1520-5126 .- 0002-7863. ; 127:14, s. 5043-5048
  • Tidskriftsartikel (refereegranskat)abstract
    • Supported phospholipid bilayers (SPBs) have emerged as important model systems for studies of the natural cell membrane and its components, which are essential for the integrity and function of cells in all living organisms, and also constitute common targets for therapeutic drugs and in disease diagnosis. However, the preferential occurrence of spontaneous SPB formation on silicon-based substrates, but not on bare noble-metal surfaces, has so far excluded the use of the localized surface plasmon resonance (LSPR) sensing principle for studies of lipid-membrane-mediated biorecognition reactions. This is because the LSPR phenomenon is associated with, and strongly confined to, the interfacial region of nanometric noble-metal particles. This problem has been overcome in this study by a self-assembly process utilizing localized rupture of phospholipid vesicles on silicon dioxide in the bottom of nanometric holes in a thin gold film. The hole-induced localization of the LSPR field to the voids of the holes is demonstrated to provide an extension of the LSPR sensing concept to studies of reactions confined exclusively to SPB-patches supported on SiO2. In particular, we emphasize the possibility of performing label-free studies of lipid-membrane-mediated reaction kinetics, including the compatibility of the assay with array-based reading (similar to 7 x 7 mu m(2)) and detection of signals originating from bound protein in the zeptomole regime.
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  • Dalby, Matthew J, et al. (författare)
  • Nanomechanotransduction and interphase nuclear organization influence on genomic control.
  • 2007
  • Ingår i: Journal of Cellular Biochemistry. - : Wiley. - 0730-2312 .- 1097-4644. ; 102:5, s. 1234-44
  • Tidskriftsartikel (refereegranskat)abstract
    • The ability of cells to alter their genomic regulation in response to mechanical conditioning or through changes in morphology and the organization of the interphase nuclei are key questions in cell biology. Here, two nanotopographies have been used as a model surfaces to change cell morphology in order to investigate spatial genomic changes within the nuclei of fibroblasts. Initially, centromeres for chromosome pairs were labeled and the average distance on different substrates calculated. Further to this, Affymetrix whole genome GeneChips were used to rank genomic changes in response to topography and plot the whereabouts on the chromosomes these changes were occurring. It was seen that as cell spreading was changed, so were the positions along the chromosomes that gene regulations were being observed. We hypothesize that as changes in cell and thus nuclear morphology occur, that this may alter the probability of transcription through opening or closing areas of the chromosomes to transcription factors.
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  • Resultat 1-10 av 22
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Sutherland, Duncan, ... (13)
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