| 1. |
- Bozóky, Benedek, et al.
(författare)
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Novel signatures of cancer-associated fibroblasts
- 2013
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Ingår i: International Journal of Cancer. - : Wiley. - 0020-7136 .- 1097-0215. ; 133:2, s. 286-293
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Tidskriftsartikel (refereegranskat)abstract
- Increasing evidence indicates the importance of the tumor microenvironment, in particular cancer-associated fibroblasts, in cancer development and progression. In our study, we developed a novel, visually based method to identify new immunohistochemical signatures of these fibroblasts. The method employed a protein list based on 759 protein products of genes identified by RNA profiling from our previous study, comparing fibroblasts with differential growth-modulating effect on human cancers cells, and their first neighbors in the human protein interactome. These 2,654 proteins were analyzed in the Human Protein Atlas online database by comparing their immunohistochemical expression patterns in normal versus tumor-associated fibroblasts. Twelve new proteins differentially expressed in cancer-associated fibroblasts were identified (DLG1, BHLHE40, ROCK2, RAB31, AZI2, PKM2, ARHGAP31, ARHGAP26, ITCH, EGLN1, RNF19A and PLOD2), four of them can be connected to the Rho kinase signaling pathway. They were further analyzed in several additional tumor stromata and revealed that the majority showed congruence among the different tumors. Many of them were also positive in normal myofibroblast-like cells. The new signatures can be useful in immunohistochemical analysis of different tumor stromata and may also give us an insight into the pathways activated in them in their true in vivo context. The method itself could be used for other similar analysis to identify proteins expressed in other cell types in tumors and their surrounding microenvironment.
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| 2. |
- Felföldi, Tamás, et al.
(författare)
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Polyphasic bacterial community analysis of an aerobic activated sludge removing phenols and thiocyanate from coke plant effluent
- 2010
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Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 101:10, s. 3406-3414
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Tidskriftsartikel (refereegranskat)abstract
- Biological purification processes are effective tools in the treatment of hazardous wastes such as toxic compounds produced in coal coking. In this study, the microbial community of a lab-scale activated sludge system treating coking effluent was assessed by cultivation-based (strain isolation and identification, biodegradation tests) and culture-independent techniques (sequence-aided T-RFLP, taxon-specific PCR). The results of the applied polyphasic approach showed a simple microbial community dominated by easily culturable heterotrophic bacteria. Comamonas badia was identified as the key microbe of the system, since it was the predominant member of the bacterial community, and its phenol degradation capacity was also proved. Metabolism of phenol, even at elevated concentrations (up to 1500 mg/L), was also presented for many other dominant (Pseudomonas, Rhodanobacter, Oligella) and minor (Alcaligenes, Castellaniella, Microbacterium) groups, while some activated sludge bacteria (Sphingomonas, Rhodopseudomonas) did not tolerate it even in lower concentrations (250 mg/L). In some cases, closely related strains showed different tolerance and degradation properties. Members of the genus Thiobacillus were detected in the activated sludge, and were supposedly responsible for the intensive thiocyanate biodegradation observed in the system. Additionally, some identified bacteria (e.g. C. badia and the Ottowia-related strains) might also have had a significant impact on the structure of the activated sludge due to their floc-forming abilities. © 2009 Elsevier Ltd. All rights reserved.
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| 3. |
- Küpper, Clemens, et al.
(författare)
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Heterozygosity-fitness correlations of conserved microsatellite markers in Kentish plovers Charadrius alexandrinus
- 2010
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Ingår i: Molecular Ecology. - 1365-294X. ; 19:23, s. 5172-5185
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Tidskriftsartikel (refereegranskat)abstract
- Abstract Heterozygosity-fitness correlations (HFCs) are frequently used to examine the relationship between genetic diversity and fitness. Most studies have reported positive HFCs, although there is a strong bias towards investigating HFCs in genetically impoverished populations. We investigated HFCs in a large genetically diverse breeding population of Kentish plovers Charadrius alexandrinus in southern Turkey. This small shorebird exhibits highly variable mating and care systems, and it is becoming an ecological model species to understand breeding system evolution. Using 11 conserved and six anonymous microsatellite markers, we tested whether and how heterozygosity was associated with chick survival, tarsus and body mass growth controlling for nongenetic effects (chick sex, hatching date, length of biparental care and site quality) that influence survival and growth. There was no genome-wide effect of heterozygosity on fitness, and we did not find any significant effects of heterozygosity on growth rates. However, two of the 11 conserved markers displayed an association with offspring survival: one marker showed a positive HFC, whereas the other marker showed a negative HFC. Heterozygosity at three further conserved loci showed significant interaction with nongenetic variables. In contrast, heterozygosity based on anonymous microsatellite loci was not associated with fitness or growth. Markers that were correlated with chick survival were not more likely to be located in exons or introns than other markers that lacked this association.
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| 4. |
- Székely, Éva, et al.
(författare)
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Synthesizing expressive speech from amateur audiobook recordings
- 2012
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Ingår i: Spoken Language Technology Workshop (SLT). ; , s. 297-302
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Konferensbidrag (refereegranskat)abstract
- Freely available audiobooks are a rich resource of expressive speech recordings that can be used for the purposes of speech synthesis. Natural sounding, expressive synthetic voices have previously been built from audiobooks that contained large amounts of highly expressive speech recorded from a profes- sionally trained speaker. The majority of freely available au- diobooks, however, are read by amateur speakers, are shorter and contain less expressive (less emphatic, less emotional, etc.) speech both in terms of quality and quantity. Synthesiz- ing expressive speech from a typical online audiobook there- fore poses many challenges. In this work we address these challenges by applying a method consisting of minimally su- pervised techniques to align the text with the recorded speech, select groups of expressive speech segments and build expres- sive voices for hidden Markov-model based synthesis using speaker adaptation. Subjective listening tests have shown that the expressive synthetic speech generated with this method is often able to produce utterances suited to an emotional mes- sage. We used a restricted amount of speech data in our exper- iment, in order to show that the method is generally applicable to most typical audiobooks widely available online.
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