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A new enzymatic ass...
A new enzymatic assay to quantify inorganic pyrophosphate in plasma
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- Lundkvist, Stefan (författare)
- Uppsala universitet,Institutionen för kemi - BMC,Thomas Jefferson Univ, Sidney Kimmel Med Coll, Jefferson Inst Mol Med, Dept Dermatol & Cutaneous Biol, 233 S 10th St, Philadelphia, PA 19107 USA.;Thomas Jefferson Univ, Sidney Kimmel Med Coll, PXE Int Ctr Excellence Res & Clin Care, 233 S 10th St, Philadelphia, PA 19107 USA.
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- Niaziorimi, Fatemeh (författare)
- Thomas Jefferson Univ, Sidney Kimmel Med Coll, Jefferson Inst Mol Med, Dept Dermatol & Cutaneous Biol, 233 S 10th St, Philadelphia, PA 19107 USA.;Thomas Jefferson Univ, Sidney Kimmel Med Coll, PXE Int Ctr Excellence Res & Clin Care, 233 S 10th St, Philadelphia, PA 19107 USA.
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- Szeri, Flora (författare)
- Thomas Jefferson Univ, Sidney Kimmel Med Coll, Jefferson Inst Mol Med, Dept Dermatol & Cutaneous Biol, 233 S 10th St, Philadelphia, PA 19107 USA.;Thomas Jefferson Univ, Sidney Kimmel Med Coll, PXE Int Ctr Excellence Res & Clin Care, 233 S 10th St, Philadelphia, PA 19107 USA.;Inst Enzymol, Res Ctr Nat Sci, Budapest, Hungary.;Semmelweis Univ, Dept Biochem, Budapest, Hungary.
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- Caffet, Matthew (författare)
- PXE Int, Damascus, MD USA.
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- Terry, Sharon F. (författare)
- PXE Int, Damascus, MD USA.
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- Johansson, Gunnar (författare)
- Uppsala universitet,Institutionen för kemi - BMC
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- Jansen, Robert S. (författare)
- Radboud Univ Nijmegen, Dept Microbiol, Nijmegen, Netherlands.
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- van de Wetering, Koen (författare)
- Thomas Jefferson Univ, Sidney Kimmel Med Coll, Jefferson Inst Mol Med, Dept Dermatol & Cutaneous Biol, 233 S 10th St, Philadelphia, PA 19107 USA.;Thomas Jefferson Univ, Sidney Kimmel Med Coll, PXE Int Ctr Excellence Res & Clin Care, 233 S 10th St, Philadelphia, PA 19107 USA.
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(creator_code:org_t)
- 2022-11-19
- 2023
- Engelska.
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Ingår i: Analytical and Bioanalytical Chemistry. - : Springer Nature. - 1618-2642 .- 1618-2650. ; 415:3, s. 481-492
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Inorganic pyrophosphate (PPi) is a crucial extracellular mineralization regulator. Low plasma PPi concentrations underlie the soft tissue calcification present in several rare hereditary mineralization disorders as well as in more common conditions like chronic kidney disease and diabetes. Even though deregulated plasma PPi homeostasis is known to be linked to multiple human diseases, there is currently no reliable assay for its quantification. We here describe a PPi assay that employs the enzyme ATP sulfurylase to convert PPi into ATP. Generated ATP is subsequently quantified by firefly luciferase-based bioluminescence. An internal ATP standard was used to correct for sample-specific interference by matrix compounds on firefly luciferase activity. The assay was validated and shows excellent precision (< 3.5%) and accuracy (93-106%) of PPi spiked into human plasma samples. We found that of several anticoagulants tested only EDTA effectively blocked conversion of ATP into PPi in plasma after blood collection. Moreover, filtration over a 300,000-Da molecular weight cut-off membrane reduced variability of plasma PPi and removed ATP present in a membrane-enclosed compartment, possibly platelets. Applied to plasma samples of wild-type and Abcc6(-/-) rats, an animal model with established low circulating levels of PPi, the new assay showed lower variability than the assay that was previously in routine use in our laboratory. In conclusion, we here report a new and robust assay to determine PPi concentrations in plasma, which outperforms currently available assays because of its high sensitivity, precision, and accuracy.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Pyrophosphate
- Ectopic mineralization
- ATP sulfurylase
- Plasma
- Vascular calcification
- Mineralization inhibitor
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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