SwePub
Sök i SwePub databas

  Utökad sökning

Träfflista för sökning "WFRF:(Szponar Bogumila) srt2:(2002)"

Sökning: WFRF:(Szponar Bogumila) > (2002)

  • Resultat 1-2 av 2
Sortera/gruppera träfflistan
   
NumreringReferensOmslagsbildHitta
1.
  • Szponar, Bogumila, et al. (författare)
  • Limitations in the use of 3-hydroxy fatty acid analysis to determine endotoxin in mammalian samples
  • 2002
  • Ingår i: Journal of Microbiological Methods. - 1872-8359 .- 0167-7012. ; 50:3, s. 283-289
  • Tidskriftsartikel (refereegranskat)abstract
    • 3-Hydroxy fatty acids (3-OH FAs) of 10-18-carbon chain lengths are constituents of the lipopolysaccharide of Gram-negative bacteria. These acids are used as chemical markers for determining endotoxin in environmental samples. The present communication addresses the question whether this type of analysis also would be applicable to mammalian samples. Low levels (6.1 +/- 1.6-94.0 +/- 23.2 pmol/ml) of the studied 3-OH FAs were detected in blood from both conventional and germ-fine rats. The levels were considerably higher (0.0-1.06 +/- 0.17 nmol/mg) in livers. The amounts of the 3-OH FAs did not differ between the two groups of rats. All analyses were made by gas chromatography-tandem mass spectrometry (GC-MSMS) for unequivocal identification. The results illustrate a limitation in using 3-OH FA analysis to determine endotoxin in mammalian samples since these acids may represent not only endotoxin but also products from mammalian mitochondrial fatty acid beta-oxidation. (C) 2002 Elsevier Science B.V. All rights reserved.
  •  
2.
  • Vanitha, JD, et al. (författare)
  • Identification of a group of nontuberculous mycobacteria isolated from the South Indian BCG trial area by HPLC
  • 2002
  • Ingår i: Current Science. - 0011-3891. ; 82:2, s. 189-191
  • Tidskriftsartikel (refereegranskat)abstract
    • Twenty-five isolates of nontuberculous mycobacteria isolated from the South Indian BCG trial area were analysed by high performance liquid chromatography (HPLC) for mycolic acid pattern. The chromatograms differentiated the isolates into four species, namely M. terrae complex, M. intracellulare, M. parafortuitum and M. fortuitum. Three strains were unidentified, one of which did not show any mycolic acid peaks. All isolates had been identified as M. diernhoferi by biochemical methods in a previous study. Nineteen of the isolates were analysed by gas chromatography-mass spectrometry (GC-MS) for the presence of tuberculostearic acid, 2-eicosanol and mycolic acid cleavage products, and were classified as nonchromogens or rapidly growing mycobacteria. The results show that HPLC can discriminate the described mycobacterial species better than biochemical methods and GC-MS.
  •  
Skapa referenser, mejla, bekava och länka
  • Resultat 1-2 av 2
Typ av publikation
tidskriftsartikel (2)
Typ av innehåll
refereegranskat (2)
Författare/redaktör
Larsson, Lennart (2)
Szponar, Bogumila (2)
Midtvedt, T (1)
Norin, E (1)
Vanitha, JD (1)
Immanuel, C (1)
visa fler...
Paramasivan, CN (1)
visa färre...
Lärosäte
Lunds universitet (2)
Karolinska Institutet (1)
Språk
Engelska (2)
Forskningsämne (UKÄ/SCB)
Medicin och hälsovetenskap (2)
År
2002 (2)Ta bort avgränsningen

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy