| 1. |
- Ferrando, R, et al.
(författare)
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3-Hydroxy fatty acids in saliva as diagnostic markers in chronic periodontitis
- 2005
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Ingår i: Journal of Microbiological Methods. - : Elsevier BV. - 1872-8359 .- 0167-7012. ; 62:3, s. 285-291
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Tidskriftsartikel (refereegranskat)abstract
- Saturated straight- and branched-chain 3-hydroxy fatty acids (3-OH FAs) of 10-18 carbon chain lengths were determined in saliva from 27 individuals with chronic periodontitis and 18 healthy individuals by using gas chromatography-tandem mass spectrometry. Of the 14 different 3-OH FAs detected, 3-OH-C-i17:0 was the most abundant in the periodontitis samples while 3-OH-C-14:0 was the most abundant in the healthy individuals. Considering the relative percentages of 3-OH-C-12:0, 3-OH-C-14:0, 3OH-C-i17:0, and 3-OH-C-17:0, 95.6% of all cases were correctly classified as healthy individuals or periodontitis patients by means of discriminant analysis. The sensitivity, specificity, positive predictive value and negative predictive value of 3-OH FA analysis in diagnosing peridontitis were, respectively, 0.92, 1.00, 1.00, and 0.90. The results indicate that 3-OH FA analysis of saliva samples is a useful diagnostic method in chronic periodontitis.
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| 2. |
- Larsson, L, et al.
(författare)
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Tobacco smoke as a major source of endotoxin in indoor air
- 2005
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Ingår i: Indoor Air 2005: Proceedings of the 10th International Conference on Indoor Air Quality and Climate, Vols 1-5. ; , s. 1655-1658
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Konferensbidrag (refereegranskat)abstract
- Mass spectrometry was used to determine the endotoxin (lipopolysaccharide, LPS) marker 3-hydroxytetradecanoic acid and 3-deoxy -D-manno-2-octulosonic acid in cigarette tobacco and smoke particles. Tobacco from cigarettes of different brands purchased in four different countries in Europe and Asia contained similar amounts of LPS. Indoor exposure to environmental tobacco smoke (ETS) in rooms of smoking students entailed inhalation of LPS in amounts that were 6-60 times higher than in student rooms without ongoing smoking. The fact that cigarette smoke contains large amounts of endotoxin may partly explain the high prevalence of respiratory disorders among smokers and may also draw attention to a hitherto unknown or neglected risk factor of ETS.
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| 3. |
- Sebastian, Aleksandra, et al.
(författare)
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Characterization of the microbial community in indoor environments by chemical marker analysis: an update and critical evaluation.
- 2005
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Ingår i: Indoor Air. - : Hindawi Limited. - 0905-6947 .- 1600-0668. ; 15 Suppl 9:9, s. 20-26
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Tidskriftsartikel (refereegranskat)abstract
- We published recently an integrated procedure for applying chemical marker analysis to characterize the microbiology of indoor environments comprising a scheme for extraction and analysis of markers of endotoxin, peptidoglycan/bacterial biomass, and fungal biomass. In the present paper, we report some significant improvements and also new possibilities of the described approach. We found that while 3-hydroxy fatty acids (3-OH FAs) of 10-14 carbon chain lengths are useful endotoxin markers, longer 3-OH FAs (i.e. with 16 carbon atoms and more) may rather serve as markers of Actinobacteria. We introduced C-13-labeled 3-hydroxytridecanoic acid, from labeled Pectinatus cerevisiiphilus, as an internal standard to improve quantification of the 3-OH FAs in the gas chromatography-mass spectrometry analysis. Finally, in experiments aiming to identify a suitable method for collection of house dust for chemical marker analysis, we found that the marker compositions of dusts sedimented on plexiglass plates that were spatially well-distributed in a studied room at different heights above floor level, were undistinguishable. This type of sampling thus appears to be well suited for use, e.g. in epidemiological studies. In summary, the presented work describes important new capabilities of chemical marker analysis in defining human exposure to microorganisms in indoor environments.
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