| 1. |
- Berggård, T, et al.
(författare)
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Alpha1-microglobulin chromophores are located to three lysine residues semiburied in the lipocalin pocket and associated with a novel lipophilic compound
- 1999
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Ingår i: Protein Science. - : Wiley. - 0961-8368. ; 8:12, s. 20-2611
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Tidskriftsartikel (refereegranskat)abstract
- Alpha1-microglobulin (alpha1m) is an electrophoretically heterogeneous plasma protein. It belongs to the lipocalin superfamily, a group of proteins with a three-dimensional (3D) structure that forms an internal hydrophobic ligand-binding pocket. Alpha1m carries a covalently linked unidentified chromophore that gives the protein a characteristic brown color and extremely heterogeneous optical properties. Twenty-one different colored tryptic peptides corresponding to residues 88-94, 118-121, and 122-134 of human alpha1m were purified. In these peptides, the side chains of Lys92, Lys118, and Lys130 carried size heterogeneous, covalently attached, unidentified chromophores with molecular masses between 122 and 282 atomic mass units (amu). In addition, a previously unknown uncolored lipophilic 282 amu compound was found strongly, but noncovalently associated with the colored peptides. Uncolored tryptic peptides containing the same Lys residues were also purified. These peptides did not carry any additional mass (i.e., chromophore) suggesting that only a fraction of the Lys92, Lys118, and Lys130 are modified. The results can explain the size, charge, and optical heterogeneity of alpha1m. A 3D model of alpha1m, based on the structure of rat epididymal retinoic acid-binding protein (ERABP), suggests that Lys92, Lys118, and Lys130 are semiburied near the entrance of the lipocalin pocket. This was supported by the fluorescence spectra of alpha1m under native and denatured conditions, which indicated that the chromophores are buried, or semiburied, in the interior of the protein. In human plasma, approximately 50% of alpha1m is complex bound to IgA. Only the free alpha1m carried colored groups, whereas alpha1m linked to IgA was uncolored.
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| 2. |
- Dalemo, M., et al.
(författare)
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ORWARE – A simulation model for organic waste handling systems. : Part 1: Model description
- 1997
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Ingår i: Resources, Conservation and Recycling. - 0921-3449 .- 1879-0658. ; 21:1, s. 17-37
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Tidskriftsartikel (refereegranskat)abstract
- A simulation model, ORWARE (ORganic WAste REsearch), for the handling of organic waste in urban areas has been constructed. The model provides a comprehensive view of the environmental effects, plant nutrient utilisation and energy turnover for this large and complex system. The ORWARE model consists of several sub-models; sewage plant, incineration, landfill, compost, anaerobic digestion, truck transport, transport by sewers, residue transport and spreading of residues on arable land. The model is intended for simulating different scenarios, and the results are: emissions to air and water, energy turnover and the amount of residues returned to arable land. All results are presented, both as the gross figure for the entire system and figures for each process. Throughout the model all physical flows are described by the same variable vector, consisting of 43 substances. This extensive vector facilitates a thorough analysis of the results, but involves some difficulties in acquiring relevant data. In this paper, the model is described. Results from a hypothetical case study are presented in a companion paper.
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| 3. |
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| 4. |
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| 5. |
- Jonsson, T, et al.
(författare)
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Nonequilibrium dynamics in a three-dimensional spin glass
- 1999
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Ingår i: PHYSICAL REVIEW B-CONDENSED MATTER. - 0163-1829. ; 59:13, s. 8770-8777
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Tidskriftsartikel (refereegranskat)abstract
- Nonequilibrium dynamics in a AE(11% Mn) spin glass has been studied by low frequency ac-susceptibility and magnetic relaxation experiments. The results unequivocally show that spin structures that memorize the cooling process are imprinted in the system.
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| 6. |
- Jönsson, Anders, 1959, et al.
(författare)
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Local anesthetics improve dermal perfusion after burn injury.
- 1998
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Ingår i: The Journal of burn care & rehabilitation. - 0273-8481. ; 19:1 Pt 1, s. 50-6
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Tidskriftsartikel (refereegranskat)abstract
- Deep partial-thickness burn injury was induced in the abdominal skin of anesthetized rats. Dermal perfusion was assessed by laser Doppler flowmetry. In the first set of experiments, one group of rats (n = 15) was topically treated with a lidocaine-prilocaine cream 5% (25 mg of each in 1 g) for 6 hours, starting 5 minutes after inducing the burn injury. In one control group (n = 14), the thermal injury was treated with placebo cream. Results showed a markedly reduced perfusion in the skin of the control animals within the first hour after burn injury, with further decrease during the following 5 hours of observation. In animals treated with the lidocaine-prilocaine cream, skin perfusion in the burned area was significantly increased during the first 30 minutes after the burn injury compared to before the burn (p < 0.01), followed by a decrease to a level below the preburn stage but significantly higher than that of control animals during the first hour after burn injury (p < 0.05). As opposed to burned control animals, skin perfusion gradually recovered toward preburn levels at the end of the experiment in local anesthetic-treated animals. In the second experimental set, four groups of animals were burned and subsequently treated with a bolus dose of lidocaine intravenously (2 mg/kg), followed by continuous intravenous lidocaine infusions at a rate of 50 (n = 10), 100 (n = 11), or 150 (n = 10) micrograms.kg-1.min-1. The infusions were started 5 minutes after the burn injury and lasted for 6 hours. Corresponding volumes of saline solution were given to burned control animals (n = 10). Results showed a significantly improved skin perfusion in the lidocaine-treated group in a dose-response fashion as compared to control animals. A maximum improvement of dermal perfusion in the burned area was induced by intravenous lidocaine at an infusion rate of 150 micrograms.kg-1.min-1 as compared to burned controls treated with isotonic saline solution infusions (p < 0.01). Results showed that topical or systemic administration of local anesthetics can prevent progressive dermal ischemia after thermal injury.
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| 7. |
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| 8. |
- Jönsson, Pär, et al.
(författare)
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Heat and metal transfer in gas metal arc welding using argon and helium
- 1995
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Ingår i: Metallurgical and materials transactions. B, process metallurgy and materials processing science. - 1073-5615 .- 1543-1916. ; 26:2, s. 383-395
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Tidskriftsartikel (refereegranskat)abstract
- This article describes a theoretical investigation on the arc parameters and metal transfer in gas metal arc welding (GMAW) of mild steel using argon and helium shielding gases. Major differences in the predicted arc parameters were determined to be due to large differences in thermophysical properties. Various findings from the study include that an arc cannot be struck in a pure helium atmosphere without the assistance of metal vapor, that a strong electromagnetic cathode force affects the fluid flow and heat transfer in the helium arc, providing a possible explanation for the experimentally observed globular transfer mode and that the tapering of the electrode in an argon arc is caused by electron condensation on the side of the electrode. © 1995 The Minerals, Metals & Material Society.
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| 9. |
- Jönsson, Åsa, 1965, et al.
(författare)
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Life cycle assessment of flooring materials: Case study
- 1997
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Ingår i: Building and Environment. - 0360-1323. ; 32:3, s. 245-255
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Tidskriftsartikel (refereegranskat)abstract
- The environmental impact of the three flooring materials linoleum, vinyl flooring and solid wood flooring during their life cycles was assessed and compared through life cycle assessment (LCA). The scenarios used describe a Swedish situation. Only impacts on the natural environment were studied The quantitative results of the inventory analysis were evaluated by using three different assessment methods. According to the results, solid wood flooring proved to be clearly the most environmentally sound flooring. Linoleum was ranked as more environmentally sound than vinyl flooring, although this was less evident in comparison with ranking the solid wood flooring.
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| 10. |
- Nilsson, T, et al.
(författare)
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Differences in co-variation of inorganic elements in the bulk and surface of human deciduous enamel: an induction analysis study.
- 1998
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Ingår i: Connective tissue research. - 0300-8207. ; 38:1-4
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Tidskriftsartikel (refereegranskat)abstract
- This paper demonstrates a method for determination of co-variation between some inorganic elements in the bulk and surface areas of human tooth enamel. The technique is based on a computerised induction analysis of data obtained by secondary ion mass spectrometry (SIMS). When comparing the present data with an earlier study from our laboratory, it became evident that with only a moderate increase in the amount of data for the induction analysis, the results increased very considerably in reliability and precision. The patterns of co-variation between different elements differed between the bulk- and surface enamel. Only Mg expressed a similar pattern. In the bulk enamel the elements Na and P expressed a high degree of co-variation. Similarly, Mg and C expressed a high degree of co-variation. Mg was an element often found to co-vary with bulk enamel elements. In the enamel surface, F and Cl co-varied. In addition, Cl was an element often found to co-vary with other enamel surface elements.
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