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- Ikoma, T., et al.
(författare)
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Protein adsorption on hydroxyapatite nanosensors with different crystal sizes studied in situ by a quartz crystal microbalance with the dissipation method
- 2009
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Ingår i: Journal of the American Ceramic Society. - : Wiley. - 0002-7820 .- 1551-2916. ; 92:5, s. 1125-1128
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Tidskriftsartikel (refereegranskat)abstract
- Hydroxyapatite (HAp) nanocrystals with different crystal sizes were deposited by the electrophoretic deposition method on the gold surface of a quartz crystal microbalance with a dissipation probe. The nanosensors formed this way were used to elucidate the adsorption mechanism of proteins with a similar pI value. The crystal sizes and the area of the a-plane affected only the adsorption amount of human serum albumin, but not that of bovine plasma fibrinogen. The viscoelastic property, Delta D/Delta f, of each absorbed layer on the nanosensors was almost constant. The protein adsorption mechanism can be explained as follows: the dissociated carboxyl groups (negative charge) of albumin were interacted with calcium ions and the hydrated amine groups (positive charge) at the alpha C domain of fibrinogen were with phosphate ions on the HAp surface.
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