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| 2. |
- Ellouze, C., et al.
(författare)
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Nucleotide Cofactor-Dependent Structural Change of Xenopus laevis Rad51 Protein Filament Detected by Small-Angle Neutron Scattering Measurements in Solution
- 1997
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Ingår i: Biochemistry. - : American Chemical Society (ACS). - 1520-4995 .- 0006-2960. ; 36:44, s. 13524-13529
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Tidskriftsartikel (refereegranskat)abstract
- Rad51 protein, a eukaryotic homologue of RecA protein, forms a filamentous complex with DNA and catalyzes homologous recombination. We have analyzed the structure of Xenopus Rad51 protein (XRad51.1) in solution by small-angle neutron scattering (SANS). The measurements showed that XRad51.1 forms a helical filament independently of DNA. The sizes of the cross-sectional and helical pitch of the filament could be determined, respectively, from a Guinier plot and the position of the subsidiary maximum of SANS data. We observed that the helical structure is modified by nucleotide binding as in the case of RecA. Upon ATP binding under high-salt conditions (600 mM NaCl), the helical pitch of XRad51.1 filament was increased from 8 to 10 nm and the cross-sectional diameter decreased from 7 to 6 nm. The pitch sizes of XRad51.1 are similar to, though slightly larger than, those of RecA filament under corresponding conditions. A similar helical pitch size was observed by electron microscopy for budding yeast Rad51 [Ogawa, T., et al. (1993) Science 259, 1896-1899]. In contrast to the RecA filament, the structure of XRad51.1 filament with ADP is not significantly different from that with ATP. Thus, the hydrolysis of ATP to ADP does not modify the helical filament of XRad51.1. Together with our recent observation that ADP does not weaken the XRad51.1/DNA interaction, the effect of ATP hydrolysis on XRad51.1 nucleofilament should be very different from that on RecA.
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| 3. |
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| 4. |
- Butorin, S. M., et al.
(författare)
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Resonant inelastic soft X-ray scattering at the 4d edge of Ce-based heavy fermion materials
- 1999
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Ingår i: Journal of Electron Spectroscopy and Related Phenomena. - 0368-2048 .- 1873-2526. ; 101-103, s. 783-786
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Tidskriftsartikel (refereegranskat)abstract
- Resonant X-ray scattering measurements were performed on CeB6, CeAl, γ-Ce, and α-Ce at various incident-photon energies near the Ce 4d threshold. A pronounced inelastic scattering structure which has 4f character is observed at about 4 eV below the elastic peak. The structure shows a distinct resonant behavior as well as a dependence on the degree of 4f hybridization and can therefore be attributed to charge-transfer excitations to the 4f0 state. The intensity of the elastic peak increases when going from the systems with low Kondo temperature TK to those with high TK which is consistent with a Kondo scale behavior. By analyzing the scattering data, a controversial issue on the validity of a single-impurity Anderson model in heavy-fermion materials is addressed.
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| 7. |
- Schmidtke, A, et al.
(författare)
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Suicide rates in the world:: Update
- 1999
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Ingår i: ARCHIVES OF SUICIDE RESEARCH. - 1381-1118. ; 5:1, s. 81-89
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 8. |
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| 9. |
- Butorin, SM, et al.
(författare)
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Resonant inelastic soft-X-ray scattering at the 4d edge of Ce-based heavy-fermion materials
- 1999
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Ingår i: JOURNAL OF ELECTRON SPECTROSCOPY AND RELATED PHENOMENA. - 0368-2048. ; 103, s. 783-786
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Resonant X-ray scattering measurements were performed on CeB6, CeAl, gamma-Ce, and alpha-Ce at various incident-photon energies near the Ce 4d threshold. A pronounced inelastic scattering structure which has 4f character is observed at about 4 eV below th
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| 10. |
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