| 1. |
- He, Shu-Lan, et al.
(författare)
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Mitochondrial-related gene expression profiles suggest an important role of PGC-1alpha in the compensatory mechanism of endemic dilated cardiomyopathy.
- 2013
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Ingår i: Experimental Cell Research. - : Elsevier. - 0014-4827 .- 1090-2422. ; 319:17, s. 2604-2616
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Tidskriftsartikel (refereegranskat)abstract
- Keshan disease (KD) is an endemic dilated cardiomyopathy with unclear etiology. In this study, we compared mitochondrial-related gene expression profiles of peripheral blood mononuclear cells (PBMCs) derived from 16 KD patients and 16 normal controls in KD areas. Total RNA was isolated, amplified, labeled and hybridized to Agilent human 4 × 44k whole genome microarrays. Mitochondrial-related genes were screened out by the Third-Generation Human Mitochondria-Focused cDNA Microarray (hMitChip3). Quantitative real-time PCR, immunohistochemical and biochemical parameters related mitochondrial metabolism were conducted to validate our microarray results. In KD samples, 34 up-regulated genes (ratios ≥ 2.0) were detected by significance analysis of microarrays and ingenuity systems pathway analysis (IPA). The highest ranked molecular and cellular functions of the differentially regulated genes were closely related to amino acid metabolism, free radical scavenging, carbohydrate metabolism, and energy production. Using IPA, 40 significant pathways and four significant networks, involved mainly in apoptosis, mitochondrion dysfunction, and nuclear receptor signaling were identified. Based on our results, we suggest that PGC-1alpha regulated energy metabolism and anti-apoptosis might play an important role in the compensatory mechanism of KD. Our results may lead to the identification of potential diagnostic biomarkers for KD in PBMCs, and may help to understand the pathogenesis of KD.
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| 2. |
- Liu, J., et al.
(författare)
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Finite element modeling of a non-isothermal superplastic-like forming process
- 2010
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Ingår i: AIP Conference Proceedings. - Paris : AIP. - 0094-243X. ; , s. 377-382
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Konferensbidrag (refereegranskat)abstract
- Conventional superplastic forming (SPF) has been modified to increase the productivity and reduce some of the drawbacks, such as high forming temperature and high percentage thinning, to suit the automotive industries. One of the modifications was to combine between the conventional SPF and the use of a mechanical preformed blank to form the non-superplastic grade aluminum alloy (AA5083-O). The requirement of high temperature usually results in microstructural defects during forming process. In this paper, finite element modeling was adopted to investigate the superplastic-like forming process using the non-isothermal heating system. In the simulation, two phases (mechanical pre-forming and gas blow for ming) of the process were conducted under different temperatures, where the material was mechanically drawn into the die cavity at 200°C in the first phase, and it formed with gas pressure applied at a global temperature increasing from 400°C to 500°C. Because of the non-isothermal heating of material, it was found that it had enough ductility to flow more easily in the specific zones (die corners and radius). Additionally, FEM results showed that a better formed part can be obtained by the increasing temperature forming, compared to the stable temperature phase. © 2010 American Institute of Physics.
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| 3. |
- Liu, J., et al.
(författare)
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Formability in AA5083 and AA6061 alloys for light weight applications
- 2010
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Ingår i: Materials & Design. - : Elsevier BV. - 0261-3069. ; 31:SUPPL. 1, s. S66-S70
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Tidskriftsartikel (refereegranskat)abstract
- With recent development in the automotive and aerospace industries, the lightweight, yet inexpensive aluminum alloys are of great demand in industrial applications. In this paper, considering the limited applications in non-superplastic materials, two typical alloys of AA5083 and AA6061 were investigated and compared during high temperature tensile tests to study their formability. The results of tensile tests and microstructures were shown, which indicated the deformation properties under different conditions. Both alloys exhibited relatively weak strain hardening effects especially at relatively lower strain rates. Furthermore, the highest strain rate sensitivity index (m value) was obtained, and the peak of percent elongation-to-failure also coincided with the ranges of highest m value. The flow stress coupled with the dynamic grain growth was related with the temperatures and strain rates. The grains appeared to be coarser in the deformed samples. Cavitation and recrystallization have also been found as a result of strain rate and temperature. © 2009 Elsevier Ltd.
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| 4. |
- Liu, J., et al.
(författare)
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Investigation of process parameters in superplastic forming of mechanical pre-formed sheet by FEM
- 2010
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Ingår i: Key Engineering Materials. - 1013-9826 .- 1662-9795. ; 447-448, s. 437-441
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Tidskriftsartikel (refereegranskat)abstract
- Conventional superplastic forming has been applied in automotive and aerospace industries for a few decades. Recently, superplastic forming combined with mechanical pre-forming process has been reported to be capable of forming non-superplastic AA5083 at 400°C to a surface expansion of 200 % [1]. In this paper, finite element modeling (FEM) was used to develop the combined forming process by using the non-superplastic material AA5083-O. The simulation follows the experimental sequence and was divided into two phases (mechanical pre-forming and superplastic forming). A conventional creep equation based on tensile test data was adopted as a material model for the simulation. The pressure cycle and forming time was simulated according to the actual process route. The thickness distributions obtained from simulation validated the capability of the model to be used for this case. The influence of different parameters, such as holder force, friction, and punch depth was investigated by comparing the final sheet thickness and level of material draw-in. It was found that the punch depth played a significant effect on the uniformity of thickness distribution, from which a more uniform formed part can be obtained by using the punch with higher depth during mechanical pre-forming phase. © (2010) Trans Tech Publications.
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| 5. |
- Liu, J., et al.
(författare)
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Superplastic-like forming of non-superplastic AA5083 combined with mechanical pre-forming
- 2011
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Ingår i: The International Journal of Advanced Manufacturing Technology. - : Springer Science and Business Media LLC. - 0268-3768 .- 1433-3015. ; 52:1-4, s. 123-129
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Tidskriftsartikel (refereegranskat)abstract
- Superplastic forming has been considered as an attractive process in the automotive and aerospace industries. However, the disadvantages of slow forming rate, high-temperature requirement, poor thickness distribution, and expensive base material have hindered its widespread use for high production volume. In this paper, the non-superplastic grade of 5083 aluminum alloy (AA5083) sheets with thickness of 3 mm was employed in a superplastic-like forming process, which is a combination of drawing (mechanical pre-forming) and superplastic forming (blow forming). Experimental trials were conducted to verify the possibility of improving the forming rate and lowering the process temperature. The blank was firstly pre-formed during the mechanical pre-forming phase. As a result, some part of material along the flange area was introduced inside the deformation cavity in advance of the blow forming phase. Secondly, argon gas was applied on the sheet, which would be deformed to come into contact with the inner die surface at the end of pressure cycle. It took only 8 min for the blow forming phase, and the process achieved an almost fully formed part at 400°C. The minimum thickness occurred at the inward corners, and the maximum thinning of the formed part was 54%. Grain growth and cavitation were found from the microstructure observations.
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| 6. |
- Wang, Zhaoming, et al.
(författare)
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Imputation and subset-based association analysis across different cancer types identifies multiple independent risk loci in the TERT-CLPTM1L region on chromosome 5p15.33
- 2014
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Ingår i: Human Molecular Genetics. - : Oxford University Press (OUP). - 0964-6906 .- 1460-2083. ; 23:24, s. 6616-6633
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Tidskriftsartikel (refereegranskat)abstract
- Genome-wide association studies (GWAS) have mapped risk alleles for at least 10 distinct cancers to a small region of 63 000 bp on chromosome 5p15.33. This region harbors the TERT and CLPTM1L genes; the former encodes the catalytic subunit of telomerase reverse transcriptase and the latter may play a role in apoptosis. To investigate further the genetic architecture of common susceptibility alleles in this region, we conducted an agnostic subset-based meta-analysis (association analysis based on subsets) across six distinct cancers in 34 248 cases and 45 036 controls. Based on sequential conditional analysis, we identified as many as six independent risk loci marked by common single-nucleotide polymorphisms: five in the TERT gene (Region 1: rs7726159, P = 2.10 × 10(-39); Region 3: rs2853677, P = 3.30 × 10(-36) and PConditional = 2.36 × 10(-8); Region 4: rs2736098, P = 3.87 × 10(-12) and PConditional = 5.19 × 10(-6), Region 5: rs13172201, P = 0.041 and PConditional = 2.04 × 10(-6); and Region 6: rs10069690, P = 7.49 × 10(-15) and PConditional = 5.35 × 10(-7)) and one in the neighboring CLPTM1L gene (Region 2: rs451360; P = 1.90 × 10(-18) and PConditional = 7.06 × 10(-16)). Between three and five cancers mapped to each independent locus with both risk-enhancing and protective effects. Allele-specific effects on DNA methylation were seen for a subset of risk loci, indicating that methylation and subsequent effects on gene expression may contribute to the biology of risk variants on 5p15.33. Our results provide strong support for extensive pleiotropy across this region of 5p15.33, to an extent not previously observed in other cancer susceptibility loci.
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| 7. |
- Aad, G., et al.
(författare)
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- 2014
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Ingår i: Journal of High Energy Physics. - 1029-8479 .- 1126-6708. ; :9
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Tidskriftsartikel (refereegranskat)
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| 8. |
- Aad, G., et al.
(författare)
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- 2014
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Ingår i: Journal of High Energy Physics. - 1029-8479 .- 1126-6708. ; :9
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Tidskriftsartikel (refereegranskat)
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