| 1. |
- Bekou, V, et al.
(författare)
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Detection of laminin 5-specific auto-antibodies in mucous membrane and bullous pemphigoid sera by ELISA
- 2005
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Ingår i: Journal of Investigative Dermatology. - 1523-1747. ; 124:4, s. 732-740
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Tidskriftsartikel (refereegranskat)abstract
- Mucous membrane pemphigoid (MMP) is an autoimmune bullous disease that primarily affects mucous membranes leading to a scarring phenotype. MMP patients produce auto-antibodies (auto-ab) that preferentially recognize two components of the dermoepidermal basement membrane zone (BMZ): bullous pemphigoid (BP)180 and laminin 5 (LN5). Since detection of disease-specific auto-ab may be critical for diagnosis of MMP, we developed an ELISA with affinity-purified native human LN5. A total of 24 MMP, 72 BP, and 51 control sera were analyzed for LN5-specific auto-ab: 18/24 (75.0%) MMP and 29/72 (40.3%) BP sera were LN5 reactive. Sensitivity and specificity of the LN5 ELISA for MMP were 75% and 84.3%, respectively, and 40.3% and 88.2% for BP, respectively. The LN5 ELISA was more sensitive than a dot blot assay with native LN5, which detected LN5-reactive IgG in 14/24 (58.3%) MMP and 16/72 (22.2%) BP sera. In MMP, but not BP, levels of LN5-reactive IgG correlated with disease severity. Furthermore, IgG reactivity to LN5 of the MMP and BP sera was not significantly associated with IgG reactivity against other autoantigens of the BMZ, such as BP180 or BP230. Thus, the established LN5 ELISA holds great promise as a novel diagnostic and prognostic parameter for MMP.
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| 2. |
- Khrapak, S. A., et al.
(författare)
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Particle charge in the bulk of gas discharges
- 2005
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Ingår i: Physical Review E. - 1539-3755. ; 72:1
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Tidskriftsartikel (refereegranskat)abstract
- An experimental determination of particle charge in a bulk dc discharge plasma covering a wide range of neutral gas pressures, was recently reported [S. Ratynskaia , Phys. Rev. Lett. 93, 085001 (2004)]. The charges obtained were several times smaller than the predictions of collisionless orbital motion limited theory. This discrepancy was attributed to the effect of ion-neutral collisions. In the present paper a more detailed description of this experiment is provided and additional experimental results obtained with particles of different sizes are reported. The measurements are compared with molecular dynamics simulations of particle charging for conditions similar to those of the experiment, with other available experimental data on particle charge in the bulk of gas discharges, and with a simple analytical model accounting for ion-neutral collisions. All the considered evidence indicates that ion-neutral collisions represent a very important factor, which significantly affects (reduces) the particle charge under typical discharge conditions.
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| 3. |
- Yaroshenko, V., et al.
(författare)
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Determination of the ion-drag force in a complex plasma
- 2005
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Ingår i: Physics of Plasmas. - : AIP Publishing. - 1070-664X .- 1089-7674. ; 12:9
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Tidskriftsartikel (refereegranskat)abstract
- The ion-drag force acting on dust particles in the positive column of a dc discharge is measured in the pressure range of 20-120 Pa. The force is obtained by a method which does not require a priori knowledge of the particle charge, but uses the charge gradient determined from the same experiment. The method depends only on two experimentally determined quantities: the particle drift velocity and the electric field. The comparison of experimental results with theoretical models is presented and discussed.
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| 4. |
- Yaroshenko, V. V., et al.
(författare)
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Measurements of the dust-ion momentum transfer frequency and ion drag force in complex plasmas
- 2005
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Ingår i: Contributions to Plasma Physics. - : Wiley. - 0863-1042 .- 1521-3986. ; 45:04-mar, s. 223-228
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Tidskriftsartikel (refereegranskat)abstract
- A simple self-consistent method to estimate the effect of the streaming ions on microparticles in a positive column of a dc discharge plasma is presented. The momentum transfer frequency in dust-ion collisions and the ion drag force are determined within a wide range of gas pressures, p = 20 - 120 Pa. The method does not require a priory knowledge of the particle charge, but involves only the particle charge gradient which is recovered from the same experimental data. The method depends only on two experimental quantities: dust particle drift velocity and electric field, thus minimizing the errors in the estimates. Reasonable agreement is found between our force measurements and theoretical predictions, corresponding to the regime of moderate ion-dust particle coupling.
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