| 1. |
- Rich, Rebecca L., et al.
(författare)
-
A global benchmark study using affinity-based biosensors
- 2009
-
Ingår i: Analytical Biochemistry. - : Elsevier BV. - 0003-2697 .- 1096-0309. ; 386:2, s. 194-216
-
Tidskriftsartikel (refereegranskat)abstract
- To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used.
|
|
| 2. |
- Klionsky, Daniel J., et al.
(författare)
-
Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
- 2008
-
Ingår i: Autophagy. - : Landes Bioscience. - 1554-8627 .- 1554-8635. ; 4:2, s. 151-175
-
Forskningsöversikt (refereegranskat)abstract
- Research in autophagy continues to accelerate,1 and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.2,3 There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.
|
|
| 3. |
- Krebs, Frederik C, et al.
(författare)
-
A round robin study of flexible large-area roll-to-roll processed polymer solar cell modules
- 2009
-
Ingår i: SOLAR ENERGY MATERIALS AND SOLAR CELLS. - : Elsevier BV. - 0927-0248. ; 93:11, s. 1968-1977
-
Tidskriftsartikel (refereegranskat)abstract
- A round robin for the performance of roll-to-roll coated flexible large-area polymer solar-cell modules involving 18 different laboratories in Northern America, Europe and Middle East is presented. The study involved the performance measurement of the devices at one location (Riso DTU) followed by transportation to a participating laboratory for performance measurement and return to the starting location (Riso DTU) for re-measurement of the performance. It was found possible to package polymer solar-cell modules using a flexible plastic barrier material in such a manner that degradation of the devices played a relatively small role in the experiment that has taken place over 4 months. The method of transportation followed both air-mail and surface-mail paths.
|
|
| 4. |
- Angelis, Jannis, et al.
(författare)
-
Product and service complexity and high performance work practices in the aerospace industry
- 2007
-
Ingår i: Journal of Industrial Relations. - : SAGE Publications. - 0022-1856 .- 1472-9296. ; 49:5, s. 775-781
-
Tidskriftsartikel (refereegranskat)abstract
- Offering products or services with high specification and complexity is often used as a competitive business strategy for organizations that cannot compete on cost. Theories on high performance work practices suggest that the combination of high employee involvement and aligned human resources and industrial relations allows firms to achieve higher performance. This study explores the adoption of such work practices in the UK aerospace industry. The results are based on responses from 225 firms across the value chain, ranging from service providers and systems integrators to component and material suppliers. The study identifies relevant work practices at each level of product and service complexity and discusses the varying needs firms have depending on their product offerings, and the implications on their work practices.
|
|
| 5. |
- Angelis, Jannis, et al.
(författare)
-
Product and service complexity and high performing aerospace organizations
- 2005
-
Ingår i: Proceedings of the 16<sup>th</sup> Annual Production and Operations Management Society Conference.
-
Konferensbidrag (refereegranskat)abstract
- Offering products or services with high specification and complexity is often used as a competitive business strategy for organizations that cannot compete on cost. Theories on high performance work practices suggest that the combination of high employee involvement and aligned human resources and industrial relations allows firms to achieve higher performance. This study explores the adoption of such work practices in the UK aerospace industry. The results are based on responses from 225 firms across the value chain, ranging from service providers and systems integrators to component and material suppliers. The study identifies relevant work practices at each level of product and service complexity and discusses the varying needs firms have depending on their product offerings, and the implications on their work practices.
|
|
| 6. |
|
|
| 7. |
- Baker, Naomi L., et al.
(författare)
-
Molecular consequences of dominant Bethlem myopathy collagen VI mutations
- 2007
-
Ingår i: Annals of Neurology. - : Wiley. - 1531-8249 .- 0364-5134. ; 62:4, s. 390-405
-
Tidskriftsartikel (refereegranskat)abstract
- Objective: Dominant mutations in the three collagen VI genes cause Bethlem myopathy, a disorder characterized by proximal muscle weakness and commonly contractures of the fingers, wrists, and ankles. Although more than 20 different dominant mutations have been identified in Bethlem myopathy patients, the biosynthetic consequences of only a subset of these have been studied, and in many cases, the pathogenic mechanisms remain unknown. Methods: We have screened fourteen Bethlem myopathy patients for collagen VI mutations and performed detailed analyses of collagen VI biosynthesis and intracellular and extracellular assembly. Results: Collagen VI abnormalities were identified in eight patients. One patient produced around half the normal amount of alpha 1(VI) messenger RNA and reduced amounts of collagen VI protein. Two patients had a previously reported mutation causing skipping of COL6A1 exon 14, and three patients had novel mutations leading to in-frame deletions toward the N-terminal end of the triple-helical domain. These mutations have different and complex effects on collagen VI intracellular and extracellular assembly. Two patients had single amino acid substitutions in the A-domains of COL6A2 and COL6A3. Collagen VI intracellular and extracellular assembly was normal in one of these patients. Interpretation: The key to dissecting the pathogenic mechanisms of collagen VI mutations lies in detailed analysis of collagen VI biosynthesis and assembly. The majority of mutations result in secretion and deposition of structurally abnormal collagen VI. However, one A-domain mutation had no detectable effect on assembly, suggesting that it acts by compromising collagen VI interactions in the extracellular matrix of muscle.
|
|
| 8. |
- Espinoza, Fredrik, et al.
(författare)
-
Intrusiveness management for focused, efficient, and enjoyable activities
- 2007
-
Ingår i: The Disappearing Computer. - Berlin / Heidelberg : Springer. - 9783540727255 ; , s. 143-157
-
Bokkapitel (refereegranskat)abstract
- When technologies for distributed activities develop, in particular the rapidly developing mobile technology, a larger part of our time will be spent connected to our various distributed contexts. When we meet physically we bring technology, both artifacts and services, which enable us to participate in these non-local contexts. Potentially this is a threat to focused and efficient activities due to the intrusiveness of the technology. Our aim is to contribute to the restoration of a number of the desirable properties of traditional local technology-free contexts. The intrusiveness itself is caused by at least four typical phenomena that have influenced current technology: • Focus-demanding and clearly distinguishable artifacts like phones or PCs explicitly mediate interaction with the distributed context • The functionality of services is traditionally based upon the assumption that communication is a deterministic flow of passive information, which for example, does not include information of the participants´ current context • Services in general perform individually and without coordinated communication schemes • The switches between contexts introduce a high cognitive load as each distributed context typically has its own system of characteristic objects and rules. In the FEEL project, we have developed a system called “Focused, Efficient and Enjoyable Local Activities with Intrusiveness Management” (FEELIM) that constitutes an intermediate alternative between the technology-dense and technology-free environments, which addresses the problems cited above. This research is based on a collaborative and cooperative setting where problems of intrusiveness management are confounded by several users meeting and cooperating together as opposed to isolated users dealing with similar problems of interruption management (Chen 2004; Ho 2005).
|
|
| 9. |
- Hirani, Bhavna, et al.
(författare)
-
Cyclometallated iridium and platinum complexes with noninnocent ligands
- 2007
-
Ingår i: Inorganic Chemistry. - : American Chemical Society (ACS). - 1520-510X .- 0020-1669. ; 46:10, s. 3865-3875
-
Tidskriftsartikel (refereegranskat)abstract
- The electronic properties of the cyclometalated ((CN)-N-and) complexes of iridium and platinum metals with a catechol ligand have been studied experimentally and computationally. The synthesis and characterization of (p-tolylpyridine)Ir(3,5-di-tert-butylcatechol) (abbreviated Ir-sq) and (2,4-diflorophenylpyridine)Pt(3,5-di-tert-butylcatechol) (abbreviated Pt-sq) are reported along with their structural, spectral, and electrochemical properties. Reaction of the 3,5-di-tert-butylcatechol (DTBCat) ligand with the prepared cyclometalated metal complex was carried out in air in the presence of a base. The resulting complexes are air stable and are paramagnetic with the unpaired electron residing mainly on the catechol ligand. The bond lengths obtained from X-ray structure analysis and the theoretical results suggest the semiquinone form of the catechol ligand. Low-energy, intense (similar to 10(3) M-1 cm(-1)) transitions are observed in the visible to near-infrared region (600-700 nm) of the absorption spectra of the metal complexes. Electrochemically, the complexes exhibit a reversible reduction of the semiquinone form to the catechol form of the ligand and an irreversible oxidation to the unstable quinone form of the ligand. The noninnocent catechol ligand plays a significant role in the electronic properties of the metal complexes. Density functional theory (DFT) and time-dependent density functional theory (TD-DFT) calculations on the two open-shell molecules provide the ground-state and excited-state energies of the molecular orbitals involved in the observed low-energy transitions. The spin density in the two complexes resides mainly on the catechol ligand. The intense transition arises from excitation of the beta electron from a HOMO-n (n = 1 or 2 here) to the LUMO, rather than from the excitation of the unpaired alpha electron.
|
|
| 10. |
- Kuntz, Philipp, et al.
(författare)
-
More than Just the Final Straw: Stolen Elections as Revolutionary Triggers
- 2005
-
Ingår i: Conference on Authoritarian Regimes: Conditions for Stability and Change. - Istanbul : Friedrich-Alexander Universität Erlangen-Nürnberg.
-
Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Stolen elections are triggering events that overcome barriers to revolutionary action against electoral authoritarian regimes. They mobilize ordinary citizens, strengthen the opposition, and divide the regime. As neo-institutionalist theories of revolution suggest, the relative openness of electoral authoritarianism inhibits mass protest. But when elections are stolen, regimes undergo “closure,” increasing the probability of protest. The failure of other potential revolutionary precipitants underlines that stolen elections are not merely replaceable final straws. Stolen elections have not only been crucial for the emergence of revolutionary situations, they have shaped outcomes as well. Linking popular mobilization to fraudulent elections has become part of the repertoire of contention of democratic revolutionaries.
|
|
