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- Reisner, Walter, et al.
(författare)
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Single-molecule denaturation mapping of DNA in nanofluidic channels
- 2010
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Ingår i: Proceedings of the National Academy of Sciences. - : Proceedings of the National Academy of Sciences. - 1091-6490 .- 0027-8424. ; 107:30, s. 13294-13299
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Tidskriftsartikel (refereegranskat)abstract
- Here we explore the potential power of denaturation mapping as a single-molecule technique. By partially denaturing YOYO (R)-1-labeled DNA in nanofluidic channels with a combination of formamide and local heating, we obtain a sequence-dependent "barcode" corresponding to a series of local dips and peaks in the intensity trace along the extended molecule. We demonstrate that this structure arises from the physics of local denaturation: statistical mechanical calculations of sequence-dependent melting probability can predict the barcode to be observed experimentally for a given sequence. Consequently, the technique is sensitive to sequence variation without requiring enzymatic labeling or a restriction step. This technique may serve as the basis for a new mapping technology ideally suited for investigating the long-range structure of entire genomes extracted from single cells.
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- Tommerup, Henrik, et al.
(författare)
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Existing sustainable renovation concepts, Deliverable D1.1 : Successful Sustainable Renovation Business for Single-Family Houses - SuccessFamilies
- 2010
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- The Nordic single-family house renovation market is dominated by a craftsman based approach with individual solutions, traditional warehouses ”do-it-yourself-shops” and some actors marketing single products. To speed up the implementation of sustainable renovation of single-family houses there is a great need for full-service packages including consulting, contract work, follow-up, financing and operation and maintenance. There are few Nordic examples of such service models for renovation of single-family houses which entered the market recently. The success of these concepts is yet to be evaluated.One successful full-service package described in the report is a campaign by an energy company in Sweden who convinced 78% of 456 owners of houses with resistance heaters to connect to its biomass based district heating network. The campaign was successful because of its package offer and information provision with emphasis on economic aspects and functional reliability.The existing technical renovation concepts, typically focussing on application of only a few of the available technical solutions, have not been successful in realizing large scale energy efficiency gains. Renovation of single-family houses might be carried out based on design solutions with good combinations of the possible range of technical solutions including e.g. “passive (insulation) measures” and “solar measures” in order to reach a low primary energy level. Key aspects of reaching a low primary energy level in connection with renovation are described in the form of typical energy renovation measures and technical principles of low energy renovation including recommended extent of measures.Full service and technical renovation concepts should make it easy, simple and secure for the consumer to invest in a low energy renovation of their house. The building sector needs easy to use knowledge and initiatives which ensures that they can offer solutions which fulfil the demand for quality, economy and a simple process. To speed up the implementation of sustainable renovation of single-family houses, society needs to stimulate the process including better incentives structures, e.g. increased tax on energy and/or subsidy programmes. Combined with an outlook for rising global energy prices, sustainable renovation of single-family houses then has the potential to become an important market area in the future.
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