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- Akdis, M, et al.
(författare)
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Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cells
- 2004
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Ingår i: Journal of Experimental Medicine. - : Rockefeller University Press. - 0022-1007 .- 1540-9538. ; 199:11, s. 1567-1575
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Tidskriftsartikel (refereegranskat)abstract
- The mechanisms by which immune responses to nonpathogenic environmental antigens lead to either allergy or nonharmful immunity are unknown. Single allergen-specific T cells constitute a very small fraction of the whole CD4(+) T cell repertoire and can be isolated from the peripheral blood of humans according to their cytokine profile. Freshly purified interferon-gamma-, interleukin (IL)-4-, and IL-10-producing allergen-specific CD4(+) T cells display characteristics of T helper cell (Th)1-, Th2, and T regulatory (Tr)1-like cells, respectively. Tr1 cells consistently represent the dominant subset specific for common environmental allergens in healthy individuals; in contrast, there is a high frequency of allergen-specific IL-4-secreting T cells in allergic individuals. Tr1 cells use multiple suppressive mechanisms, IL-10 and TGF-beta as secreted cytokines, and cytotoxic T lymphocyte antigen 4 and programmed death 1 as surface molecules. Healthy and allergic individuals exhibit all three allergen-specific subsets in different proportions, indicating that a change in the dominant subset may lead to allergy development or recovery. Accordingly, blocking the suppressor activity of Tr1 cells or increasing Th2 cell frequency enhances allergen-specific Th2 cell activation ex vivo. These results indicate that the balance between allergen-specific Tr1 cells and Th2 cells may be decisive in the development of allergy.
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| 7. |
- Valenta, J., et al.
(författare)
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Active planar optical waveguide made from luminescent silicon nanocrystals
- 2003
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Ingår i: Applied Physics Letters. - : AIP Publishing. - 0003-6951 .- 1077-3118. ; 82:6, s. 955-957
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Tidskriftsartikel (refereegranskat)abstract
- We show experimentally that a layer of silicon nanocrystals, prepared by the Si-ion implantation (with the energy of 400 keV) into a synthetic silica slab and exhibiting room-temperature red photoluminescence, can serve simultaneously as a single-mode planar optical waveguide. The waveguide is shown to self-select guided transverse electric and transverse magnetic modes from the broad photoluminescence emission of the nanocrystals resulting in a substantially narrower emission spectrum for these modes. We further report on an investigation of optical gain in a sample implanted to a dose of 4x10(17) cm(-2). Despite the occurrence of strong waveguiding, results of the variable stripe length method turned out not to be able to give unambiguous evidence for optical gain.
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| 8. |
- Valenta, J., et al.
(författare)
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Photonic band-gap effects on photoluminescence of silicon nanocrystals embedded in artificial opals
- 2003
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Ingår i: Journal of Applied Physics. - : AIP Publishing. - 0021-8979 .- 1089-7550. ; 93:8, s. 4471-4474
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Tidskriftsartikel (refereegranskat)abstract
- Si nanocrystals were formed in synthetic opals by Si-ion implantation and their optical properties studied using microphotoluminescence and reflection techniques. The properties of areas with high crystalline quality are compared with those of disordered regions of samples. The photoluminescence spectrum from Si nanocrystals embedded in silica spheres is narrowed by the inhibition of emission at wavelengths corresponding to the opal photonic pseudoband gap (similar to690 nm). Measurements of photoluminescence spectra from individual implanted silica spheres is also demonstrated and the number of emitting Si nanocrystals in single brightly emitting spheres is estimated to be of the order of one thousand.
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- van Hage-Hamsten, M, et al.
(författare)
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Nasal challenges with recombinant derivatives of the major birch pollen allergen Bet v 1 induce fewer symptoms and lower mediator release than rBet v 1 wild-type in patients with allergic rhinitis
- 2002
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Ingår i: Clinical and Experimental Allergy. - : Wiley. - 1365-2222 .- 0954-7894. ; 32:10, s. 1448-1453
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Tidskriftsartikel (refereegranskat)abstract
- Background Genetic engineering of the major birch pollen allergen (Bet v 1) has led to the generation of recombinant Bet v 1 derivatives with markedly reduced IgE-binding capacity, but with retained T cell activating ability. Objective To compare the mucosal reactivity to rBet v 1 derivatives with rBet v 1 wild-type as basis for new therapeutic strategies for birch pollen allergy based on mucosal tolerance induction. Methods Outside the pollen season, 10 patients with birch pollen allergic rhinitis and mild asthma underwent four nasal challenge-sessions in a randomized, double-blind, and cross-over design, employing increasing doses of rBet v 1 fragment mix, rBet v 1 trimer, rBet v 1 wild-type and diluent (albumin). Nasal lavage fluids (NAL) were collected before the challenge-series as well as 10 min, 4 and 24 h thereafter. Nasal lavage fluid levels of tryptase as well as EPO and ECP were measured as indices of mast cell and eosinophil activity, respectively. Results All 10 patients tolerated the highest accumulated dose, 8.124 mug, when challenged with rBet v 1 trimer, eight with rBet v 1 fragments compared to one when challenged with rBet v 1 wild-type. No late phase reactions were observed. The change in tryptase levels (pre-challenge vs. 10 min) was significantly lower after challenges with rBet v 1 trimer and rBet v 1 fragments than with rBet v 1 wild-type. The change in EPO/ECP concentration pre-challenge versus 4 h post-challenge was lower for rBet v 1 trimer and the change was significantly lower when pre-challenge versus 24 h post-challenge to rBet v 1 fragments and rBet v 1 wild-type was examined. Conclusion The derivatives induced significantly fewer symptoms and lower mast cell and eosinophil activation than rBet v 1 wild-type upon application to the nasal mucosa. They could in the future be candidates for immunotherapy based on mucosal tolerance induction.
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