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Träfflista för sökning "WFRF:(Vanherberghen Bruno) srt2:(2009)"

Sökning: WFRF:(Vanherberghen Bruno) > (2009)

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1.
  • Benninger, Richard K. P., et al. (författare)
  • Live Cell Linear Dichroism Imaging Reveals Extensive Membrane Ruffling within the Docking Structure of Natural Killer Cell Immune Synapses
  • 2009
  • Ingår i: Biophysical Journal. - : Elsevier BV. - 0006-3495 .- 1542-0086. ; 96:2, s. L13-L15
  • Tidskriftsartikel (refereegranskat)abstract
    • We have applied fluorescence imaging of two-photon linear dichroism to measure the subresolution organization of the cell membrane during formation of the activating (cytolytic) natural killer (NK) cell immune synapse (IS). This approach revealed that the NK cell plasma membrane is convoluted into ruffles at the periphery, but not in the center of a mature cytolytic NK cell IS. Time-lapse imaging showed that the membrane ruffles formed at the initial point of contact between NK cells and target cells and then spread radialy across the intercellular contact as the size of the IS increased, becoming absent from the center of the mature synapse. Understanding the role of such extensive membrane ruff ling in the assembly of cytolytic synapses is an intriguing new goal.
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2.
  • Manneberg, Otto, et al. (författare)
  • Flow-free transport of cells in microchannels by frequency-modulated ultrasound
  • 2009
  • Ingår i: Lab on a Chip. - 1473-0197 .- 1473-0189. ; 9, s. 833-837
  • Tidskriftsartikel (refereegranskat)abstract
    • We demonstrate flow-free transport of cells and particles by the use of frequency-modulated ultrasonic actuation of a microfluidic chip. Two different modulation schemes are combined: A rapid (1 kHz) linear frequency sweep around similar to 6.9 MHz is used for two-dimensional spatial stabilization of the force field over a 5 mm long inlet channel of constant cross section, and a slow (0.2-0.7 Hz) linear frequency sweep around similar to 2.6 MHz is used for flow-free ultrasonic transport and positioning of cells or particles. The method is used for controlling the motion and position of cells monitored with high-resolution optical microscopy, but can also be used more generally for improving the robustness and performance of ultrasonic manipulation micro-devices.
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  • Resultat 1-3 av 3

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