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E-cadherin: a diffe...
E-cadherin: a differentiation marker in thyroid malignancies.
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Brabant, G (författare)
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Hoang-Vu, C (författare)
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Cetin, Y (författare)
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visa fler...
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Dralle, H (författare)
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Scheumann, G (författare)
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- Mölne, Johan, 1958 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin , Avdelningen för patologi,Institute of Laboratory Medicine, Dept of Pathology
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Hansson, G (författare)
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Jansson, S (författare)
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Ericson, L E (författare)
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Nilsson, M (författare)
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visa färre...
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(creator_code:org_t)
- 1993
- 1993
- Engelska.
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Ingår i: Cancer research. - 0008-5472. ; 53:20, s. 4987-93
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Loss of E-cadherin (uvomorulin), a Ca(2+)-dependent cell adhesion molecule required for normal epithelial function, has been attributed a pathogenetic role in tumor invasion. The expression of E-cadherin was studied in normal and neoplastic follicular epithelium of the human thyroid by Northern blot analysis and immunofluorescence on frozen tissue sections. In the normal thyroid (n = 10) and in benign thyroid disorders (n = 21; toxic diffuse goitre; multinodular goitre; follicular adenomas), E-cadherin mRNA levels were equally high and the follicles were generally stained, mainly along the lateral surface of the epithelial cells, by the anti-E-cadherin monoclonal antibody. In anaplastic thyroid carcinomas (n = 6) E-cadherin expression was very low or lacking. In papillary carcinomas (n = 23), E-cadherin mRNA levels varied from nearly normal to highly reduced, which roughly correlated with the overall immunofluorescence intensity. However, the immunostaining also revealed a heterogeneous "all-or-nothing" expression of E-cadherin among adjacent cells in the same tumor. In the follicular carcinomas (n = 9), E-cadherin mRNA levels were in general rather high but the immunostaining varied considerably. A few papillary and follicular tumors lacked immunoreactive E-cadherin in spite of high mRNA levels. In oxyphilic (Hürthle) cell tumors, comprising both adenomas (n = 4) and carcinomas (n = 2), E-cadherin immunoreactivity was reduced and distributed intracellularly rather than at the cell surface. The expression of E-cadherin in relapsing thyroid carcinomas and in tumors with metastatic spreading was, irrespective of the histiotype, low or lacking. Sequential Northern analysis revealed a close correlation between the expression levels of E-cadherin and the thyrotropin receptor. Together, the data suggest that in human thyroid malignancies both gene expression and posttranscriptional control of E-cadherin may be impaired.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Nyckelord
- Adenoma
- metabolism
- pathology
- Biomarkers
- Tumor
- analysis
- Blotting
- Northern
- Cadherins
- analysis
- biosynthesis
- Carcinoma
- Papillary
- metabolism
- pathology
- Cell Differentiation
- Fluorescent Antibody Technique
- Humans
- RNA
- Messenger
- analysis
- metabolism
- Receptors
- Thyrotropin
- biosynthesis
- Thyroid Gland
- cytology
- metabolism
- Thyroid Neoplasms
- metabolism
- pathology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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Brabant, G
-
Hoang-Vu, C
-
Cetin, Y
-
Dralle, H
-
Scheumann, G
-
Mölne, Johan, 19 ...
-
visa fler...
-
Hansson, G
-
Jansson, S
-
Ericson, L E
-
Nilsson, M
-
visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Endokrinologi oc ...
- Artiklar i publikationen
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Cancer research
- Av lärosätet
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Göteborgs universitet