Dynamic behavior analysis of single cells using droplet microfluidics

• We present a droplet microfluidic platform to automatically track and characterize the behavior of single cells over time. Time series analysis of single cells was enabled by encapsulating the cells in microdroplets followed by trapping microdroplets in static array of microwells that were fabricated on chip to make the droplets addressable by their position and imaging them over time. In this paper, we demonstrate the potential of automated time-lapse imaging and image analysis approach in droplet microfluidics by studying the viability of large number of single cells over time.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

Automatic tracking

Cell viability

Droplet microfluidics

Fluorescent imaging

Time series analysis

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