| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Niemi, MEK, et al.
(författare)
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- 2021
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swepub:Mat__t
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| 3. |
- Lind, Lars, et al.
(författare)
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Heterogeneous contributions of change in population distribution of body mass index to change in obesity and underweight NCD Risk Factor Collaboration (NCD-RisC)
- 2021
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Ingår i: eLife. - : eLife Sciences Publications Ltd. - 2050-084X. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- From 1985 to 2016, the prevalence of underweight decreased, and that of obesity and severe obesity increased, in most regions, with significant variation in the magnitude of these changes across regions. We investigated how much change in mean body mass index (BMI) explains changes in the prevalence of underweight, obesity, and severe obesity in different regions using data from 2896 population-based studies with 187 million participants. Changes in the prevalence of underweight and total obesity, and to a lesser extent severe obesity, are largely driven by shifts in the distribution of BMI, with smaller contributions from changes in the shape of the distribution. In East and Southeast Asia and sub-Saharan Africa, the underweight tail of the BMI distribution was left behind as the distribution shifted. There is a need for policies that address all forms of malnutrition by making healthy foods accessible and affordable, while restricting unhealthy foods through fiscal and regulatory restrictions.
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| 4. |
- Mishra, A, et al.
(författare)
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Diminishing benefits of urban living for children and adolescents' growth and development
- 2023
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 1476-4687 .- 0028-0836. ; 615:7954, s. 874-883
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Tidskriftsartikel (refereegranskat)abstract
- Optimal growth and development in childhood and adolescence is crucial for lifelong health and well-being1–6. Here we used data from 2,325 population-based studies, with measurements of height and weight from 71 million participants, to report the height and body-mass index (BMI) of children and adolescents aged 5–19 years on the basis of rural and urban place of residence in 200 countries and territories from 1990 to 2020. In 1990, children and adolescents residing in cities were taller than their rural counterparts in all but a few high-income countries. By 2020, the urban height advantage became smaller in most countries, and in many high-income western countries it reversed into a small urban-based disadvantage. The exception was for boys in most countries in sub-Saharan Africa and in some countries in Oceania, south Asia and the region of central Asia, Middle East and north Africa. In these countries, successive cohorts of boys from rural places either did not gain height or possibly became shorter, and hence fell further behind their urban peers. The difference between the age-standardized mean BMI of children in urban and rural areas was <1.1 kg m–2 in the vast majority of countries. Within this small range, BMI increased slightly more in cities than in rural areas, except in south Asia, sub-Saharan Africa and some countries in central and eastern Europe. Our results show that in much of the world, the growth and developmental advantages of living in cities have diminished in the twenty-first century, whereas in much of sub-Saharan Africa they have amplified.
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| 5. |
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| 6. |
- Taddei, C, et al.
(författare)
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Repositioning of the global epicentre of non-optimal cholesterol
- 2020
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 1476-4687 .- 0028-0836. ; 582:7810, s. 73-
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Tidskriftsartikel (refereegranskat)abstract
- High blood cholesterol is typically considered a feature of wealthy western countries1,2. However, dietary and behavioural determinants of blood cholesterol are changing rapidly throughout the world3 and countries are using lipid-lowering medications at varying rates. These changes can have distinct effects on the levels of high-density lipoprotein (HDL) cholesterol and non-HDL cholesterol, which have different effects on human health4,5. However, the trends of HDL and non-HDL cholesterol levels over time have not been previously reported in a global analysis. Here we pooled 1,127 population-based studies that measured blood lipids in 102.6 million individuals aged 18 years and older to estimate trends from 1980 to 2018 in mean total, non-HDL and HDL cholesterol levels for 200 countries. Globally, there was little change in total or non-HDL cholesterol from 1980 to 2018. This was a net effect of increases in low- and middle-income countries, especially in east and southeast Asia, and decreases in high-income western countries, especially those in northwestern Europe, and in central and eastern Europe. As a result, countries with the highest level of non-HDL cholesterol—which is a marker of cardiovascular risk—changed from those in western Europe such as Belgium, Finland, Greenland, Iceland, Norway, Sweden, Switzerland and Malta in 1980 to those in Asia and the Pacific, such as Tokelau, Malaysia, The Philippines and Thailand. In 2017, high non-HDL cholesterol was responsible for an estimated 3.9 million (95% credible interval 3.7 million–4.2 million) worldwide deaths, half of which occurred in east, southeast and south Asia. The global repositioning of lipid-related risk, with non-optimal cholesterol shifting from a distinct feature of high-income countries in northwestern Europe, north America and Australasia to one that affects countries in east and southeast Asia and Oceania should motivate the use of population-based policies and personal interventions to improve nutrition and enhance access to treatment throughout the world.
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| 7. |
- Kanai, M, et al.
(författare)
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- 2023
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swepub:Mat__t
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| 8. |
- Yang (杨轶), Yi, et al.
(författare)
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The interaction of supernova 2018evt with a substantial amount of circumstellar matter – An SN 1997cy-like event
- 2023
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Ingår i: Monthly notices of the Royal Astronomical Society. - : Oxford University Press (OUP). - 0035-8711 .- 1365-2966. ; 519:2, s. 1618-1647
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Tidskriftsartikel (refereegranskat)abstract
- A rare class of supernovae (SNe) is characterized by strong interaction between the ejecta and several solar masses of circumstellar matter (CSM) as evidenced by strong Balmer-line emission. Within the first few weeks after the explosion, they may display spectral features similar to overluminous Type Ia SNe, while at later phase their observation properties exhibit remarkable similarities with some extreme case of Type IIn SNe that show strong Balmer lines years after the explosion. We present polarimetric observations of SN 2018evt obtained by the ESO Very Large Telescope from 172 to 219 d after the estimated time of peak luminosity to study the geometry of the CSM. The non-zero continuum polarization decreases over time, suggesting that the mass-loss of the progenitor star is aspherical. The prominent H α emission can be decomposed into a broad, time-evolving component and an intermediate-width, static component. The former shows polarized signals, and it is likely to arise from a cold dense shell (CDS) within the region between the forward and reverse shocks. The latter is significantly unpolarized, and it is likely to arise from shocked, fragmented gas clouds in the H-rich CSM. We infer that SN 2018evt exploded inside a massive and aspherical circumstellar cloud. The symmetry axes of the CSM and the SN appear to be similar. SN 2018evt shows observational properties common to events that display strong interaction between the ejecta and CSM, implying that they share similar circumstellar configurations. Our preliminary estimate also suggests that the circumstellar environment of SN 2018evt has been significantly enriched at a rate of ∼0.1 M⊙ yr−1 over a period of >100 yr.
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| 9. |
- Gou, De Hai, et al.
(författare)
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Inhibition of copper transporter 1 prevents α-synuclein pathology and alleviates nigrostriatal degeneration in AAV-based mouse model of Parkinson's disease
- 2021
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Ingår i: Redox Biology. - : Elsevier BV. - 2213-2317. ; 38
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Tidskriftsartikel (refereegranskat)abstract
- The formation of α-synuclein aggregates is a major pathological hallmark of Parkinson's disease. Copper promotes α-synuclein aggregation and toxicity in vitro. The level of copper and copper transporter 1, which is the only known high-affinity copper importer in the brain, decreases in the substantia nigra of Parkinson's disease patients. However, the relationship between copper, copper transporter 1 and α-synuclein pathology remains elusive. Here, we aim to decipher the molecular mechanisms of copper and copper transporter 1 underlying Parkinson's disease pathology. We employed yeast and mammalian cell models expressing human α-synuclein, where exogenous copper accelerated intracellular α-synuclein inclusions and silencing copper transporter 1 reduced α-synuclein aggregates in vitro, suggesting that copper transporter 1 might inhibit α-synuclein pathology. To study our hypothesis in vivo, we generated a new transgenic mouse model with copper transporter 1 conditional knocked-out specifically in dopaminergic neuron. Meanwhile, we unilaterally injected adeno-associated viral human-α-synuclein into the substantia nigra of these mice. Importantly, we found that copper transporter 1 deficiency significantly reduced S129-phosphorylation of α-synuclein, prevented dopaminergic neuronal loss, and alleviated motor dysfunction caused by α-synuclein overexpression in vivo. Overall, our data indicated that inhibition of copper transporter 1 alleviated α-synuclein mediated pathologies and provided a novel therapeutic strategy for Parkinson's disease and other synucleinopathies.
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| 10. |
- Chen, Kun-Chih (jimmy), et al.
(författare)
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A NoC-based simulator for design and evaluation of deep neural networks
- 2020
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Ingår i: Microprocessors and microsystems. - : ELSEVIER. - 0141-9331 .- 1872-9436. ; 77
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Tidskriftsartikel (refereegranskat)abstract
- The astonishing development in the field of artificial neural networks (ANN) has brought significant advancement in many application domains, such as pattern recognition, image classification, and computer vision. ANN imitates neuron behaviors and makes a decision or prediction by learning patterns and features from the given data set. To reach higher accuracies, neural networks are getting deeper, and consequently, the computation and storage demands on hardware platforms are steadily increasing. In addition, the massive data communication among neurons makes the interconnection more complex and challenging. To overcome these challenges, ASIC-based DNN accelerators are being designed which usually incorporate customized processing elements, fixed interconnection, and large off-chip memory storage. As a result, DNN computation involves large memory accesses due to frequent load/off-loading data, which significantly increases the energy consumption and latency. Also, the rigid architecture and interconnection among processing elements limit the efficiency of the platform to specific applications. In recent years, Network-on-Chip-based (NoC-based) DNN becomes an emerging design paradigm because the NoC interconnection can help to reduce the off-chip memory accesses while offers better scalability and flexibility. To evaluate the NoC-based DNN in the early design stage, we introduce a cycle-accurate NoC-based DNN simulator, called DNNoC-sim. To support various operations such as convolution and pooling in the modern DNN models, we first propose a DNN flattening technique to convert diverse DNN operation into MAC-like operations. In addition, we propose a DNN slicing method to evaluate the large-scale DNN models on a resource-constraint NoC platform. The evaluation results show a significant reduction in the off-chip memory accesses compared to the state-of-the-art DNN model. We also analyze the performance and discuss the trade-off between different design parameters.
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