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Sökning: WFRF:(Wenk Markus R.) > (2016) > Characterization of...

Characterization of a dynamic metabolon producing the defense compound dhurrin in sorghum

Laursen, Tomas (författare)
Plant Biochemistry Laboratory, Department of Plant and Environmental Science, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.;bioSYNergy, Center for Synthetic Biology, DK-1871 Frederiksberg C, Denmark.;VILLUM Research Center for Plant Plasticity, DK-1871 Frederiksberg C, Denmark.;Copenhagen Plant Science Center, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.;Feedstocks Division, Joint BioEnergy Institute, Emeryville, CA 94608, USA.
Borch, Jonas (författare)
bioSYNergy, Center for Synthetic Biology, DK-1871 Frederiksberg C, Denmark.;VILLUM Center For Bioanalytical Sciences, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark.
Knudsen, Camilla (författare)
Plant Biochemistry Laboratory, Department of Plant and Environmental Science, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.;bioSYNergy, Center for Synthetic Biology, DK-1871 Frederiksberg C, Denmark.;VILLUM Research Center for Plant Plasticity, DK-1871 Frederiksberg C, Denmark.;Copenhagen Plant Science Center, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.
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Bavishi, Krutika (författare)
Plant Biochemistry Laboratory, Department of Plant and Environmental Science, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.;bioSYNergy, Center for Synthetic Biology, DK-1871 Frederiksberg C, Denmark.;VILLUM Research Center for Plant Plasticity, DK-1871 Frederiksberg C, Denmark.;Copenhagen Plant Science Center, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.
Torta, Federico (författare)
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Singapore.
Martens, Helle Juel (författare)
Copenhagen Plant Science Center, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.
Silvestro, Daniele (författare)
Copenhagen Plant Science Center, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.
Hatzakis, Nikos S. (författare)
bioSYNergy, Center for Synthetic Biology, DK-1871 Frederiksberg C, Denmark.;Department of Chemistry, Nano-Science Center, University of Copenhagen, DK-2100 Copenhagen, Denmark.
Wenk, Markus R. (författare)
Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Singapore.;Department of Biological Sciences, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Singapore.
Dafforn, Timothy R. (författare)
School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK.;Department of Business, Energy and Industrial Strategy, Her Majesty’s Government, UK.
Olsen, Carl Erik (författare)
Plant Biochemistry Laboratory, Department of Plant and Environmental Science, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.;bioSYNergy, Center for Synthetic Biology, DK-1871 Frederiksberg C, Denmark.;VILLUM Research Center for Plant Plasticity, DK-1871 Frederiksberg C, Denmark.
Motawia, Mohammed Saddik (författare)
Plant Biochemistry Laboratory, Department of Plant and Environmental Science, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.;bioSYNergy, Center for Synthetic Biology, DK-1871 Frederiksberg C, Denmark.;VILLUM Research Center for Plant Plasticity, DK-1871 Frederiksberg C, Denmark.;Copenhagen Plant Science Center, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.
Hamberger, Björn (författare)
Plant Biochemistry Laboratory, Department of Plant and Environmental Science, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.;bioSYNergy, Center for Synthetic Biology, DK-1871 Frederiksberg C, Denmark.
Møller, Birger Lindberg (författare)
Plant Biochemistry Laboratory, Department of Plant and Environmental Science, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.;bioSYNergy, Center for Synthetic Biology, DK-1871 Frederiksberg C, Denmark.;VILLUM Research Center for Plant Plasticity, DK-1871 Frederiksberg C, Denmark.;Copenhagen Plant Science Center, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.;Carlsberg Research Laboratory, DK-1799 Copenhagen V, Denmark.
Bassard, Jean-Etienne (författare)
Plant Biochemistry Laboratory, Department of Plant and Environmental Science, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.;bioSYNergy, Center for Synthetic Biology, DK-1871 Frederiksberg C, Denmark.;VILLUM Research Center for Plant Plasticity, DK-1871 Frederiksberg C, Denmark.;Copenhagen Plant Science Center, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.
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Plant Biochemistry Laboratory, Department of Plant and Environmental Science, University of Copenhagen, DK-1871 Frederiksberg C, Denmark;bioSYNergy, Center for Synthetic Biology, DK-1871 Frederiksberg C, Denmark.;VILLUM Research Center for Plant Plasticity, DK-1871 Frederiksberg C, Denmark.;Copenhagen Plant Science Center, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.;Feedstocks Division, Joint BioEnergy Institute, Emeryville, CA 94608, USA. bioSYNergy, Center for Synthetic Biology, DK-1871 Frederiksberg C, Denmark.;VILLUM Center For Bioanalytical Sciences, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark. (creator_code:org_t)
American Association for the Advancement of Science, 2016
2016
Engelska.
Ingår i: Science. - : American Association for the Advancement of Science. - 0036-8075 .- 1095-9203. ; 354:6314, s. 890-893
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • Metabolic highways may be orchestrated by the assembly of sequential enzymes into protein complexes, or metabolons, to facilitate efficient channeling of intermediates and to prevent undesired metabolic cross-talk while maintaining metabolic flexibility. Here we report the isolation of the dynamic metabolon that catalyzes the formation of the cyanogenic glucoside dhurrin, a defense compound produced in sorghum plants. The metabolon was reconstituted in liposomes, which demonstrated the importance of membrane surface charge and the presence of the glucosyltransferase for metabolic channeling. We used in planta fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy to study functional and structural characteristics of the metabolon. Understanding the regulation of biosynthetic metabolons offers opportunities to optimize synthetic biology approaches for efficient production of high-value products in heterologous hosts.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

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