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- Dunham, I, et al.
(författare)
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The DNA sequence of human chromosome 22
- 1999
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 402:6761, s. 489-495
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Tidskriftsartikel (refereegranskat)
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| 7. |
- Newman, D J, et al.
(författare)
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Serum cystatin C measured by automated immunoassay : a more sensitive marker of changes in GFR than serum creatinine
- 1995
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Ingår i: Kidney International. - : Elsevier BV. - 0085-2538. ; 47, s. 312-318
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Tidskriftsartikel (refereegranskat)abstract
- Serum cystatin C has been suggested as a new marker of GFR. For the introduction of this marker into clinical use a rapid and automated method is required. We have developed and validated an assay for serum cystatin C using latex particle-enhanced immunoturbidimetry. Intra- and inter-assay precision were < 3% and < 5% across the assay range. Analytical recovery was 93 +/- 3.8% and no lack of parallelism was demonstrated. Regression analysis of a method comparison with an enzyme-enhanced radial-immunodiffusion method, gave PETIA = 0.074 + 0.93 x SRID, r = 0.98, N = 100. Inter-assay precision profiles showed cystatin C was measured with two-fold better precision than creatinine on the same analyzer. Cystatin C measurement was neither interfered with by icterus nor by hemolysis. 1/cystatin C versus 1/creatinine concentrations gave r = 0.67, N = 469. Comparison of Cr EDTA GFR with 1/cystatin C and 1/creatinine gave r = 0.81 and 0.50, respectively, N = 206. Calculating diagnostic sensitivity for abnormal GFR showed cystatin C to be significantly (P < 0.05) more sensitive than creatinine (71.4 vs. 52.4%). Cystatin C measurement using PETIA technology can be automated on the same instruments used routinely for the measurement of creatinine and offers better analytical performance and probably improved clinical sensitivity as a screening test for early renal damage.
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| 8. |
- Newman, Jeffrey D., et al.
(författare)
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Catalytic materials, membranes and fabrication technologies suitable for the construction of amperometric biosensors
- 1995
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 67:24, s. 4594-4599
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- A selection of recently available catalytic carbon powders were assessed and compared with the more conventionally used platinized material. Their suitability for incorporation in amperometric biosensors is discussed, In conjunction with this study, methods of applying membranes to the surfaces of these devices were investigated. Advanced fabrication technologies, potentially suitable for scale-up of sensor production, such as screen printing and ink-jet printing, were used for manufacture of the complete sensor structure. Hydrogen peroxide-sensing electrodes and glucose biosensors were produced as model systems, demonstrating the advantages of these approaches. The commercially available rhodinized carbon MCA4 produced a high current density at low potentials over a plateau region (300-400 mV vs SCE). In addition, direct oxidation of glucose (seen with platinized carbon) was not observed at the chosen potential of +350 mV. Further interference studies using fermentation media highlighted its suitability as an electrode material for use in complex samples. Ink-jet printing proved to be a successful method for the deposition of Nafion membranes of defined and reproducible geometry.
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| 9. |
- Prellner, Karin, et al.
(författare)
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Immunization and protection in pneumococcal otitis media studied in a rat model
- 1999
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Ingår i: Microbial Drug Resistance. - 1076-6294 .- 1931-8448. ; 5:1, s. 73-82
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Tidskriftsartikel (refereegranskat)abstract
- The recent and growing problem of bacterial resistance to common antibiotics has generated great interest in different methods for prevention of infections. The treatment of the pathogens causing upper airway infections and especially acute otitis media (AOM) is especially interesting in this context because these infections are a common cause of prescription of antibiotics all over the world. Both in AOM and recurrent AOM, Streptococcus pneumoniae, the most frequently occurring bacterium is isolated in 30-50% of all AOM attacks. In the last decade, multiresistant S. pneumoniae have emerged as a major problem. Thus, it is important to explore possibilities that immunization may protect against pneumococcal OM. In a well-defined animal model using Sprague-Dawley rats, we have investigated the effects of different routes of immunization with different antigens and whole cells. Together with otomicroscopical evaluation of middle ear (ME) status, samples for bacterial cultivation as well as for studies of histopathological changes have been collected. Antibody titers have been followed during and after pneumococcal AOM by an enzyme-linked immunosorbent assay (ELISA) method.
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