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- Bouyoucef, S E, et al.
(författare)
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Poster Session 2 : Monday 4 May 2015, 08
- 2015
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Ingår i: European Heart Journal Cardiovascular Imaging. - : Oxford University Press (OUP). - 2047-2404 .- 2047-2412. ; 16 Suppl 1
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Tidskriftsartikel (refereegranskat)
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| 6. |
- Sun, ND, et al.
(författare)
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Modified VEGF-A mRNA induces sustained multifaceted microvascular response and accelerates diabetic wound healing
- 2018
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Ingår i: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 8:1, s. 17509-
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Tidskriftsartikel (refereegranskat)abstract
- Capable of mediating efficient transfection and protein production without eliciting innate immune responses, chemically modified mRNA holds great potential to produce paracrine factors at a physiologically beneficial level, in a spatiotemporally controlled manner, and with low toxicity. Although highly promising in cardiovascular medicine and wound healing, effects of this emerging therapeutic on the microvasculature and its bioactivity in disease settings remain poorly understood. Here, we longitudinally and comprehensively characterize microvascular responses to AZD8601, a modified mRNA encoding vascular endothelial growth factor A (VEGF-A), in vivo. Using multi-parametric photoacoustic microscopy, we show that intradermal injection of AZD8601 formulated in a biocompatible vehicle results in pronounced, sustained and dose-dependent vasodilation, blood flow upregulation, and neovessel formation, in striking contrast to those induced by recombinant human VEGF-A protein, a non-translatable variant of AZD8601, and citrate/saline vehicle. Moreover, we evaluate the bioactivity of AZD8601 in a mouse model of diabetic wound healing in vivo. Using a boron nanoparticle-based tissue oxygen sensor, we show that sequential dosing of AZD8601 improves vascularization and tissue oxygenation of the wound bed, leading to accelerated re-epithelialization during the early phase of diabetic wound healing.
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| 8. |
- Leonardsson, Kjell, et al.
(författare)
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Calculation of species sensitivity values and their precision in marine benthic faunal quality indices
- 2015
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Ingår i: Marine Pollution Bulletin. - : Elsevier BV. - 0025-326X .- 1879-3363. ; 93:1-2, s. 94-102
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Tidskriftsartikel (refereegranskat)abstract
- A challenging aspect of benthic quality indices used for assessing the marine environment has been to compile reliable measures of the species' sensitivity to disturbances. Sensitivity values and their uncertainties can be calculated, but a problem to cope with is that the results may depend on the actual proportion of samples from disturbed and undisturbed environments. Here we calculated sensitivity values for each species along an artificial disturbance gradient created by bootstrapping varying numbers of samples from disturbed and undisturbed environments. The values were increasing, decreasing, or more or less constant along this gradient. The lowest value with the lowest uncertainty was adopted as the species sensitivity value. Analyses of the uncertainties indicated that the accuracy rather than the precision might be a concern. We suggest a method to exclude species for which the uncertainty is outside predefined limits as a precaution to reduce bias in the environmental status classification.
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