Sökning: WFRF:(Witham Katey)
> (2021) >
Cellular responses ...
Cellular responses at the application site of a high-density microarray patch delivering an influenza vaccine in a randomized, controlled phase I clinical trial
-
- Depelsenaire, Alexandra C. I. (författare)
- Vaxxas Pty Ltd, Australia
-
- Witham, Katey (författare)
- Vaxxas Pty Ltd, Australia
-
- Veitch, Margaret (författare)
- Univ Queensland, Australia
-
visa fler...
-
- Wells, James W. (författare)
- Univ Queensland, Australia
-
- Anderson, Christopher D. (författare)
- Linköpings universitet,Institutionen för klinisk och experimentell medicin,Medicinska fakulteten
-
- Lickliter, Jason D. (författare)
- Nucleus Network Pty Ltd, Australia
-
- Rockman, Steve (författare)
- Seqirus Pty Ltd, Australia; Univ Melbourne, Australia
-
- Bodle, Jesse (författare)
- Seqirus Pty Ltd, Australia
-
- Treasure, Peter (författare)
- Peter Treasure Stat Serv Ltd, England
-
- Hickling, Julian (författare)
- Tandem Ltd, England
-
- Fernando, Germain J. P. (författare)
- Vaxxas Pty Ltd, Australia; Univ Queensland, Australia
-
- Forster, Angus H. (författare)
- Vaxxas Pty Ltd, Australia
-
visa färre...
-
(creator_code:org_t)
- 2021-07-30
- 2021
- Engelska.
-
Ingår i: PLOS ONE. - : PUBLIC LIBRARY SCIENCE. - 1932-6203. ; 16:7
- Relaterad länk:
-
https://journals.plo...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Microarray patches (MAPs) have the potential to be a safer, more acceptable, easier to use and more cost-effective method for administration of vaccines when compared to the needle and syringe. Since MAPs deliver vaccine to the dermis and epidermis, a degree of local immune response at the site of application is expected. In a phase 1 clinical trial (ACTRN 12618000112268), the Vaxxas high-density MAP (HD-MAP) was used to deliver a monovalent, split inactivated influenza virus vaccine into the skin. HD-MAP immunisation led to significantly enhanced humoral responses on day 8, 22 and 61 compared with IM injection of a quadrivalent commercial seasonal influenza vaccine (Afluria Quadrivalent (R)). Here, the aim was to analyse cellular responses to HD-MAPs in the skin of trial subjects, using flow cytometry and immunohistochemistry. HD-MAPs were coated with a split inactivated influenza virus vaccine (A/Singapore/GP1908/2015 [H1N1]), to deliver 5 mu g haemagglutinin (HA) per HD-MAP. Three HD-MAPs were applied to the volar forearm (FA) of five healthy volunteers (to achieve the required 15 mu g HA dose), whilst five control subjects received three uncoated HD-MAPs (placebo). Local skin response was recorded for over 61 days and haemagglutination inhibition antibody titres (HAI) were assessed on days 1, 4, 8, 22, and 61. Skin biopsies were taken before (day 1), and three days after HD-MAP application (day 4) and analysed by flow-cytometry and immunohistochemistry to compare local immune subset infiltration. HD-MAP vaccination with 15 mu g HA resulted in significant HAI antibody titres compared to the placebo group. Application of uncoated placebo HD-MAPs resulted in mild erythema and oedema in most subjects, that resolved by day 4 in 80% of subjects. Active, HA-coated HD-MAP application resulted in stronger erythema responses on day 4, which resolved between days 22-61. Overall, these erythema responses were accompanied by an influx of immune cells in all subjects. Increased cell infiltration of CD3(+), CD4(+), CD8(+) T cells as well as myeloid CD11b(+) CD11c(+) and non-myeloid CD11b(-) dendritic cells were observed in all subjects, but more pronounced in active HD-MAP groups. In contrast, CD19(+)/CD20(+) B cell counts remained unchanged. Key limitations include the use of an influenza vaccine, to which the subjects may have had previous exposure. Different results might have been obtained with HD-MAPs inducing a primary immune response. In conclusion, influenza vaccine administered to the forearm (FA) using the HD-MAP was well-tolerated and induced a mild to moderate skin response with lymphocytic infiltrate at the site of application.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
-
PLOS ONE
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas
- Av författaren/redakt...
-
Depelsenaire, Al ...
-
Witham, Katey
-
Veitch, Margaret
-
Wells, James W.
-
Anderson, Christ ...
-
Lickliter, Jason ...
-
visa fler...
-
Rockman, Steve
-
Bodle, Jesse
-
Treasure, Peter
-
Hickling, Julian
-
Fernando, Germai ...
-
Forster, Angus H ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Immunologi inom ...
- Artiklar i publikationen
-
PLOS ONE
- Av lärosätet
-
Linköpings universitet