| 1. |
- Köhler, Th., et al.
(författare)
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Precision measurement of strong interaction isotope effects in antiprotonic 16O, 17O, and 18O atoms
- 1986
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Ingår i: Physics Letters B. - : Elsevier BV. - 0370-2693 .- 1873-2445. ; 176:3-4, s. 327-333
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Tidskriftsartikel (refereegranskat)abstract
- The strong-interaction effects in antiprotonic 16O, 17O, and 18O atoms were measured at the CERN antiproton facility, LEAR. The shifts ε{lunate} and the widths Γ of the 3d level were determined to be -112±20 eV (16O), -140±46 eV (17O), -195±20 eV (18O), and 495±45 eV (16O), 540±150 (17O), 640±40 eV (18O), respectively. © 1986.
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| 2. |
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| 3. |
- Rehnstam, A.-S., et al.
(författare)
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Identification of Vibrio anguillarum in fish using partial 16S rRNA sequences and a specific 16S rRNA oligonucleotide probe.
- 1989
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Ingår i: Applied and Environmental Microbiology. - 0099-2240 .- 1098-5336. ; 55:8, s. 1907-1910
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Tidskriftsartikel (refereegranskat)abstract
- 16S rRNA from seven different Vibrio anguillarum strains waspartially sequenced and compared. From this sequence informationwe could design a 25-base-long oligonucleotide and use it asa specific probe for identification of V. anguillarum. Thiswas determined by RNA-DNA colony hybridization and slot-blothybridization. Strong, specific hybridization to the probe wasobserved for all V. anguillarum strains tested. Furthermore,no cross-hybridization could be seen against five other bacterialspecies. The detection limit was 5 x 10(3) bacteria per ml.It was even possible to detect V. anguillarum, by slot-blothybridization, directly in a homogenized kidney from a fishthat had died of vibriosis. The partial sequence informationrevealed small but significant differences between strains ofthe same species. These sequence differences are sufficientlysignificant to allow serotyping on the RNA level. Comparingstrains of different serotypes revealed a 10-base and an 11-basedifference in V. anguillarum serotypes O8 and O9, respectively,in a 122-base partial sequence.
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| 4. |
- Ding, Yu-Shin, et al.
(författare)
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Synthesis of L-[5-11C]ornithine.
- 1989
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Ingår i: Journal of labelled compounds & radiopharmaceuticals. - 0362-4803 .- 1099-1344. ; 27, s. 1079-
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Tidskriftsartikel (refereegranskat)
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| 5. |
- Norquist, A., et al.
(författare)
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Protection against vibriosis and furunculosis by the use of attenuated strains of Vibrio anguillarum.
- 1989
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Ingår i: Applied and Environmental Microbiology. - 0099-2240 .- 1098-5336. ; 55:6, s. 1400-1405
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Tidskriftsartikel (refereegranskat)abstract
- The fish pathogen Vibrio anguillarum causes a lethal infectionin rainbow trout (Salmo gairdneri). Three different avirulentmutants, constructed by transposon insertion mutagenesis (VAN20and VAN70) or as antibiotic-resistant mutants (VAN1000), wereisolated by screening 200 individual isolated mutants for avirulence.When used as live vaccines, all three avirulent mutants wereable to induce protective immunity against the homologous aswell as a heterologous strain of V. anguillarum. When VAN1000was used, protective immunity could be recorded 1 week afterbath vaccination with 10(7) bacteria per ml of water for 30min. A single-dose immunization was effective for at least 12weeks. Western immunoblotting showed that strains of V. anguillarumhave antigenic determinants in common with Aeromonas strains.Therefore, we tested and confirmed that VAN1000 also was ableto induce protective immunity against challenge with Aeromonassalmonicida.
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