| 1. |
- Wang, Fei, et al.
(författare)
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Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level
- 2022
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Ingår i: Nature Communications. - : Springer Nature. - 2041-1723. ; 13:1
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Tidskriftsartikel (refereegranskat)abstract
- Pigs are valuable large animal models for biomedical and genetic research, but insights into the tissue- and cell-type-specific transcriptome and heterogeneity remain limited. By leveraging single-cell RNA sequencing, we generate a multiple-organ single-cell transcriptomic map containing over 200,000 pig cells from 20 tissues/organs. We comprehensively characterize the heterogeneity of cells in tissues and identify 234 cell clusters, representing 58 major cell types. In-depth integrative analysis of endothelial cells reveals a high degree of heterogeneity. We identify several functionally distinct endothelial cell phenotypes, including an endothelial to mesenchymal transition subtype in adipose tissues. Intercellular communication analysis predicts tissue- and cell type-specific crosstalk between endothelial cells and other cell types through the VEGF, PDGF, TGF-beta, and BMP pathways. Regulon analysis of single-cell transcriptome of microglia in pig and 12 other species further identifies MEF2C as an evolutionally conserved regulon in the microglia. Our work describes the landscape of single-cell transcriptomes within diverse pig organs and identifies the heterogeneity of endothelial cells and evolutionally conserved regulon in microglia.
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| 2. |
- Feng, Zhaoxuan, et al.
(författare)
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Carbon dot/polymer nanocomposites : From green synthesis to energy, environmental and biomedical applications
- 2021
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Ingår i: Sustainable Materials and Technologies. - : Elsevier BV. - 2214-9937. ; 29
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Tidskriftsartikel (refereegranskat)abstract
- Carbon dots (CDs), a novel family of multifunctional carbon-nanomaterials, has the potential to revolutionize several important fields supporting the transformation to sustainable bioeconomy. CDs are characterized by attractive properties such as tunable optoelectronic and fluorescence properties, low toxicity, good biocompatibility, bioactivity and superior water solubility. Diverse methods and precursors have been applied for the synthesis of CDs. The possibility to scale-up the synthesis of CDs by employing Green Chemistry principles is a current research hotspot and a prerequisite for large-scale production and applications. Incorporation of CDs into polymer matrices, is another emerging research area with wide potential application range. This approach endows additional desirable functions and enables easy handling and reuse. This review summarizes recent progress regarding the green synthesis of CDs, the fundamental strategies for the construction of CD/polymer nanocomposites and the state-of-art applications of the CD/polymer nanocomposites in the realm of energy storage, environment and biomedicine. Finally, the outlook including challenges and future potential of CDs and CD/polymer composites are discussed.
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| 3. |
- Feng, Zhaoxuan, et al.
(författare)
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Nano graphene oxide creates a fully biobased 3D-printed membrane with high-flux and anti-fouling oil/water separation performance
- 2024
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Ingår i: Chemical Engineering Journal. - : Elsevier B.V.. - 1385-8947 .- 1873-3212. ; 485
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Tidskriftsartikel (refereegranskat)abstract
- Facile fabrication of green and renewable bio-based membranes with good anti-fouling and oil/water separation performance is of great importance to solve the oil spills and industrial oily wastewater threatening the ecological environment. Here, a fully biobased oil/water separation membrane with an ordered porous structure was 3D printed ultraviolet-assisted direct ink writing. The components of the bio-ink were obtained by methacrylation of chitosan (CS) and gelatin (GEL) to synthesize methacrylated chitosan (CSMA) and methacrylated gelatin (GELMA), while the nanographene oxide (nGO) was derived from CS through a simple microwave-assisted hydrothermal carbonization followed by oxidation step. The addition of nGO boosted the printability of the bio-ink, and the anti-fouling property and water permeation flux of the printed membranes. As a result, the membrane M−CSMA/GELMA/nGO-0.7 with the optimal performance possessed a low water contact angle in air of 0°, and high underwater oil contact angle of 161.5°, demonstrating a combination of superhydrophilic and underwater superoleophobic properties. M−CSMA/GELMA/nGO-0.7 has good corrosion resistance and long service life as evidenced from the separation efficiency of n-heptane/water, which kept above 99.5 % and a high water permeation flux above 38,300 L m−2h−1 after 20 cyclic tests in the harsh aquatic conditions containing 1 M NaCl, 1 M HCl, or 1 M NaOH, respectively. This shows promising potential for real-life applications.
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| 4. |
- Feng, Zhaoxuan, et al.
(författare)
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Recent progress in the use of graphene/polymer composites to remove oil contaminants from water
- 2021
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Ingår i: Xinxing tancailiao. - : Elsevier BV. - 1007-8827. ; 36:2, s. 235-252
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Forskningsöversikt (refereegranskat)abstract
- Frequent oil spill accidents and the massive discharge of industrial oily sewage have destroyed the ecological balance and threatened marine life. Graphene (G) and graphene oxide (GO) have emerged as important materials in the field of oil/water separation because of their remarkable physicochemical properties including high specific surface area, low density, high porosity and tailorable surface functionality. To take full advantage of G and GO, their incorporation with polymers to build functional G/polymer and GO/polymer composites has recently gained increasing popularity because of their improved oil clean-up capability, outstanding mechanical performance, relatively low cost and adjustable surface chemical composition. Tremendous efforts have contributed to the development of G/polymer and GO/polymer composite oil clean-up sorbents and filtration membranes in 3D structural forms such as aerogels, foams, sponges and membranes. In this review, a comprehensive picture from the basic theory of the surface wettability to the recent advances in G/polymer and GO/polymer composite oil clean-up sorbents and filtration membranes are highlighted. The strategies for oil recovery and regeneration of the sorbents are also summarized. Current challenges and future research directions in this topic are provided, aimed at providing new perspectives for in-depth exploration in this field.
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| 5. |
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| 6. |
- Wang, Ying, et al.
(författare)
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Screening for differentially expressed circRNA between Kashin–Beck disease and osteoarthritis patients based on circRNA chips
- 2020
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Ingår i: Clinica Chimica Acta. - : Elsevier. - 0009-8981 .- 1873-3492. ; 501, s. 92-101
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Tidskriftsartikel (refereegranskat)abstract
- Objective: This research aims to explore differentially expressed circRNA between OA and KBD and potential diagnostic biomarkers.Methods: Total RNA was extracted from 5 pairs of KBD and OA knee joint cartilage specimens, and the expression of circRNAs was analyzed by Chip Scanning Analysis. The microarray data was verified by quantitative polymerase chain reaction (qRT-PCR). CircRNA-miRNA network was constructed to predict targeting microRNAs of circRNA genes. Peripheral blood samples from 25 KBD patients and 25 OA patients were collected for verification by qRT-PCR. Diagnostic value was evaluated by the area under the receiver operator characteristic (ROC) curve.Results: A total of 1627 circRNAs were differentially expressed between OA and KBD. Five bone and joint disease-related circRNAs were chosen for qRT-PCR validation. The difference in expression profile of hsa_circRNA_0020014 was confirmed by qRT-PCR, and its circRNA-miRNA regulation network was set up. The ROC curve demonstrated that hsa_circ_0020014_CBC1 in peripheral blood could distinguish patients with KBD and OA.Conclusion: The expression profiles of circRNA were significantly different between OA and KBD. hsa_circRNA_0020014 is a potential biomarker for differential diagnosis between these two diseases.
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| 7. |
- Wei, Yanan, et al.
(författare)
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A universal method for constructing high efficiency organic solar cells with stacked structures
- 2021
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Ingår i: Energy & Environmental Science. - : ROYAL SOC CHEMISTRY. - 1754-5692 .- 1754-5706. ; 14:4, s. 2314-2321
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Tidskriftsartikel (refereegranskat)abstract
- The construction of organic solar cells with stacked structures by the sequential deposition (SD) of donor and acceptor films has great potential in industrial production, as it demonstrates little dependence on the ratio of donor and acceptor materials, solvents, and additives. Herein, we present an eco-friendly solvent protection (ESP) method for the fabrication of high-performance OSCs with stacked structures. Several non-aromatic and non-halogenated solvents are employed as protective agents to build SD devices with a configuration of indium tin oxide (ITO)/poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS Clevios P VP Al 4083)/donor/protective solvent/acceptor/perylene diimide functionalized with amino N-oxide (PDINO)/Al, which shows that n-octane is the optimal choice for SD devices. Nine different SD systems including the fullerene and nonfullerene ones present comparable photovoltaic performance to their BC counterparts, which proves the universality of this ESP method. Significantly, the device of ITO/PEDOT:PSS/D18/N3/PDINO/Al with n-octane as the protective solvent achieves a maximum PCE of 17.52%, which is the record efficiency of SD devices. Furthermore, a protective factor (delta) is proposed to demonstrate the quantitative relationship between delta and PCE after experimental and theoretical investigation, which presents an idea to understand the mechanism and provides a guideline for solvent choices.
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| 8. |
- Wu, Yanan, et al.
(författare)
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Computationally efficient direction of arrival estimation using adaptive grid selection
- 2023
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Ingår i: IET Radar, Sonar and Navigation. - 1751-8784. ; 17:11, s. 1599-1611
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Tidskriftsartikel (refereegranskat)abstract
- The authors propose a computationally efficient approach to estimate the directions of arrival of far-field sources impinging on a sensor array. The proposed estimator is formed using a sparse reconstruction framework, employing a novel adaptive grid selection technique to reduce the dimensionality of the used dictionary matrix. The method further makes use of a SPICE-inspired dictionary to adaptively select an appropriate regularisation parameter and to select the active grid set. An adaptive stepsize selection strategy is also introduced to reduce computation complexity further. The proposed method allows for accurate direction-of-arrival (DOA) estimates using even a single snapshot, also from weak sources. Numerical simulations indicate that the method offers preferable performance in comparison to existing state-of-the-art DOA estimation algorithms.
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| 9. |
- Wu, Yanan, et al.
(författare)
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Estimating Weak DOA Signals Using Adaptive Grid Selection
- 2023
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Ingår i: Proceedings of the 22nd IEEE Statistical Signal Processing Workshop, SSP 2023. - 9781665452458
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Konferensbidrag (refereegranskat)abstract
- In this work, we consider the problem of estimating the directions of arrival of far-field sources impinging on a sensor array using a computationally efficient approach. A novel adaptive grid selection technique is employed to reduce the dimensionality of the used dictionary matrix. The method further makes use of a SPICE-inspired dictionary to adaptively select an appropriate regularization parameter and to select the active grid set. An adaptive stepsize selection strategy is also introduced to reduce computation complexity further. The proposed method allows for accurate DOA estimates using even a single snapshot, also from weak sources. Numerical simulations indicate that the method offers preferable performance in comparison to existing state-of-the-art DOA estimation algorithms.
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| 10. |
- Wu, Yanan, et al.
(författare)
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Super-resolution Direction of Arrival Estimation Using a Minimum Mean-Square Error Framework
- 2023
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Ingår i: Signal Processing. - 0165-1684. ; 212
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Tidskriftsartikel (refereegranskat)abstract
- This paper develops a novel sparse direction-of-arrival (DOA) estimation technique that avoids the common requirement of hyperparameters, which are typically difficult to set suitably in practice. Using the presented minimum mean-square error (MMSE) estimation framework, we propose a computationally efficient super-resolution DOA estimator that is implemented using an alternate updating of the spatial power distribution of the signals and of the dictionary matrix using a ridge regression algorithm. The regularization parameter determining the sparsity of the solution is formed from the previous spatial power distribution estimates using a SPICE-based criteria. The method employs an adaptive gridding strategy to avoid the grid mismatch problem. The computational complexity is further reduced by the use of an integrated wideband dictionary, determing the active dictionary in an iterative manner. The method offers high resolution estimates of closely spaced sources even for very low sample support (single snapshot) without assuming prior knowledge of the number of sources. Our evaluations illustrate the preferable performance of the proposed estimator as compared to various state-of-the art estimators, also indicating that the method's performance approaches the Cramér-Rao lower bound (CRB) for the examined problem as the signal-to-noise ratio (SNR) increases.
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