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- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 3. |
- Liu, Yi, et al.
(författare)
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Self-Assembly at a Macroscale Using Aerodynamics
- 2022
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Ingår i: Applied Sciences. - : MDPI AG. - 2076-3417. ; 12:15, s. 7676-7676
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Tidskriftsartikel (refereegranskat)abstract
- Intuitive self-assembly devices are of great significance to the emerging applications of self-assembly theory. In this paper, a novel intuitive device with an aerodynamic system is fabricated for the self-assembly experiment. Table tennis balls were used as the objects to be assembled during the self-assembly process. To understand more about the system, two experiments were designed—the directed assembly experiment was conducted to organize a specific structure and to explore the influences of environmental variables, and the indirect assembly experiment repeated with the “bottom-up” self-organization process and expressed the characteristics of “the optimization” and “the emergence” in the self-organization process. This article expressed a novel self-assembly approach at a macroscale and created a new choice or idea for the structural design and the optimization method.
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| 5. |
- Wu, Jinming, et al.
(författare)
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Investigation on the wave energy converter that reacts against an internal inverted pendulum
- 2022
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Ingår i: Energy. - : Elsevier. - 0360-5442 .- 1873-6785. ; 247
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Tidskriftsartikel (refereegranskat)abstract
- In this work, a wave energy converter (WEC) that reacts against an internal inverted pendulum, which works as an inertial device to provide reaction for power absorption and is potentially superior due to its natural high elevation of the internal mass compared to a normal pendulum, named IPWEC has been studied. Optimal structural configurations of the WEC have been identified by a genetic algorithm. The equations of motion have been defined and solved explicitly using a linearized model, which has been validated by experiments and a non-linearized model. When comparing IPWEC with the WEC that reacts against a normal pendulum (NPWEC), it is found that, although both WECs present almost the same wave power capture ability, IPWEC possesses several advantages in most sea states due to the naturally high elevation of the pendulum's center of gravity: (1) the pendulum mass and the angular motion amplitude of the pendulum are 35% and 50%, respectively, smaller than those of NPWEC; (2) the averaged reactive power required under complex conjugate control is 75% smaller than NPWEC; (3) the moment which holds the pendulum fixed relative to the hull in the survival mode is merely a half as large as that of NPWEC.
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