| 1. |
- Beal, Jacob, et al.
(författare)
-
Robust estimation of bacterial cell count from optical density
- 2020
-
Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
-
Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
|
|
| 2. |
- Fan, Qunping, 1989, et al.
(författare)
-
Over 14% efficiency all-polymer solar cells enabled by a low bandgap polymer acceptor with low energy loss and efficient charge separation
- 2020
-
Ingår i: Energy & Environmental Science. - : Royal Society of Chemistry. - 1754-5692 .- 1754-5706. ; 13:12, s. 5017-5027
-
Tidskriftsartikel (refereegranskat)abstract
- Obtaining both high open-circuit voltage (V-oc) and short-circuit current density (J(sc)) has been a major challenge for efficient all-polymer solar cells (all-PSCs). Herein, we developed a polymer acceptor PF5-Y5 with excellent optical absorption capability (onset extending to similar to 880 nm and maximum absorption coefficient exceeding 105 cm(-1) in a film), high electron mobility (3.18 x 10(3) cm(2) V-1 s(-1)) and high LUMO level (-3.84 eV) to address such a challenge. As a result, the PBDB-T:PF5-Y5-based all-PSCs achieved a high power conversion efficiency of up to 14.45% with both a high Voc (0.946 V) and a high Jsc (20.65 mA cm(-2)), due to the high and broad absorption coverage, small energy loss (0.57 eV) and efficient charge separation and transport in the device, which are among the best values in the all-PSC field. In addition, the all-PSC shows a similar to 15% improvement in PCE compared to its counterpart small molecule acceptor (Y5)-based device. Our results suggest that PF5-Y5 is a very promising polymer acceptor candidate for applications in efficient all-PSCs.
|
|
| 3. |
- Xia, Yuxin, et al.
(författare)
-
All-Polymer High-Performance Photodetector through Lamination
- 2020
-
Ingår i: Advanced Electronic Materials. - : WILEY. - 2199-160X. ; 6
-
Tidskriftsartikel (refereegranskat)abstract
- All-polymer and semitransparent organic photodetectors (OPDs) fabricated through lamination on flexible substrates are demonstrated. Through lamination both bilayer and bulk heterojunction (BHJ) configurations for two different all-polymer blends are easily made, and characterized in terms of detectivity, linear dynamic range (LDR), and bandwidth. These devices show high detectivity up to 10(11) Jones. The importance of measured noise rather than estimated shot noise in detectivity calculation is discussed and emphasized. With this high detectivity and with the geometric flexibility due to the polymer materials, these detectors can be attached to curved surfaces for optical detection. Their use at fingertips for heart rate sensing is demonstrated. These devices also have a very wide bandwidth of more than 300 kHz and an LDR of 75 dB. As such the, roll-to-roll compatible lamination method shows great potential for high-performance OPDs fabrication.
|
|
