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Träfflista för sökning "WFRF:(Xie Xiao) srt2:(1998-1999)"

Sökning: WFRF:(Xie Xiao) > (1998-1999)

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1.
  • Tang, Xiao-Jing, et al. (författare)
  • Polyethyleneimine-coated reticulated vitreous carbon electrode with immobilized enzymes as a substrate detector
  • 1998
  • Ingår i: Analytica Chimica Acta. - 0003-2670. ; 374:2-3, s. 185-190
  • Tidskriftsartikel (refereegranskat)abstract
    • Polyethyleneimine (PEI) was covalently coupled to carbodiimide-activated reticulated vitreous carbon (RVC). The PEI-coated RVC was activated with glutaraldehyde, and glycerol dehydrogenase and diaphorase were then immobilized. The PEI-coated RVC with immobilized enzymes functioned both as an enzyme reactor and a working electrode in an amperometric detection system where NAD+/NADH was recycled by the immobilized enzymes. The coated RVC electrode showed good properties compared to uncoated RVC, such as a long lifetime and a constant response to a series of injections in a flow-injection system, resulting in a relative standard deviation of 1.4%. The calibration graph was linear from the detection limit 0.1μM to 2mM NADH in the absence of recycling.
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2.
  • Xie, Xiao-Qi, et al. (författare)
  • AML and Ets proteins regulate the I alpha1 germ-line promoter.
  • 1999
  • Ingår i: European Journal of Immunology. - 0014-2980 .- 1521-4141. ; 29:2, s. 488-498
  • Tidskriftsartikel (refereegranskat)abstract
    • The immunoglobulin heavy chain (IgH) class switch recombination of B lymphocytes preferentially targets unrearranged IgH genes that have already been rendered transcriptionally active. Transcription of the germ-line IgH genes is controlled by intervening (I) regions upstream of their switch regions. The I alpha1 promoter activates transcription of the human germ-line C alpha1 gene for IgA1 and mediates the transforming growth factor (TGF)-beta1 responsiveness of this locus. Here we show that the I alpha1 promoter contains several binding sites for the AML/PEBP2/CBF family of transcription factors and that AML and Ets proteins are major regulators of the basal and TGF-beta-inducible promoter activity. Our data constitute a starting point for studies to elucidate the molecular mechanism by which TGF-beta regulates IgA production.
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