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Sökning: WFRF:(Xue S.) > (2000-2004)

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  • Kaminski, Clemens, et al. (författare)
  • Flame growth and wrinkling in a turbulent flow
  • 2000
  • Ingår i: Applied physics. B, Lasers and optics (Print). - : Springer Science and Business Media LLC. - 0946-2171 .- 1432-0649. ; 71:5, s. 711-716
  • Tidskriftsartikel (refereegranskat)abstract
    • High-speed planar laser-induced fluorescence (PLIF) and 3-D large eddy simulations (LES) are used to study turbulent flame kernel growth, wrinkling and the formation of separated flame pockets in methane/air mixtures. Turbulence was effected by a set of rotary fans situated in a cylindrical enclosure. Flame wrinkling was followed on sequential 2-D OH images captured at kHz repetition rates. Under stoichiometric conditions and low turbulence levels the flame kernel remains singly connected and close to spherical in shape. By increasing turbulence or reducing the stoichiometry of the mixture the formation of separated pockets could be observed and studied. The mechanisms behind these phenomena are investigated qualitatively by LES of a level-set G-equation describing the flame surface propagation in turbulent flows.
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  • Xue, Yongtao, et al. (författare)
  • A DNA microarray for fission yeast : minimal changes in global gene expression after temperature shift
  • 2004
  • Ingår i: Yeast. - : Wiley. - 0749-503X .- 1097-0061. ; 21:1, s. 25-39
  • Tidskriftsartikel (refereegranskat)abstract
    • Completion of the fission yeast genome sequence has opened up possibilities for post-genomic approaches. We have constructed a DNA microarray for genome-wide gene expression analysis in fission yeast. The microarray contains DNA fragments, PCR-amplified from a genomic DNA template, that represent >99% of the 5000 or so annotated fission yeast genes, as well as a number of control sequences. The GenomePRIDE software used attempts to design similarly sized DNA fragments corresponding to gene regions within single exons, near the 3'-end of genes that lack homology to other fission yeast genes. To validate the design and utility of the array, we studied expression changes after a 2 h temperature shift from 25degreesC to 36degreesC, conditions widely used when studying temperature-sensitive mutants. Obligingly, the vast majority of genes do not change more than two-fold, supporting the widely held view that temperature-shift experiments specifically reveal phenotypes associated with temperature-sensitive mutants. However, we did identify a small group of genes that showed a reproducible change in expression. Importantly, most of these corresponded to previously characterized heat-shock genes, whose expression has been reported to change after more extreme temperature shifts than those used here.. We conclude that the DNA microarray represents a useful resource for fission yeast researchers as well as the broader yeast community, since it will facilitate comparison with the distantly related budding yeast, Saccharomyces cerevisiae. To maximize the utility of this resource, the array and its component parts are fully described in On-line Supplementary Information and are also available commercially.
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