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Träfflista för sökning "WFRF:(Yeo J.) srt2:(1995-1999)"

Sökning: WFRF:(Yeo J.) > (1995-1999)

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  • Son, G.S., et al. (författare)
  • Binding Mode of Norfloxacin to Calf Thymus DNA
  • 1998
  • Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 1520-5126 .- 0002-7863. ; 120:26, s. 6451-6457
  • Tidskriftsartikel (refereegranskat)abstract
    • Norfloxacin, a quinolone antibacterial reagent, has been studied with respect to its binding to calf thymus DNA using fluorescence and linear dichroism techniques and unwinding of supercoiled DNA. The fluorescence of norfloxacin is strongly quenched in the presence of DNA and using this decrease in a fluorescence titration the equilibrium constant of the complex formation was established to be 2.8 x 10(3) M-1. The electric transition moments of the norfloxacin chromophore have been analyzed using fluorescence anisotropy, magnetic circular dichroism, and linear dichroism in stretched poly(vinyl alcohol) film and INDO/S calculations. These data are then used to interpret flow linear dichroism results for the norfloxacin-DNA complex. The transition moments for the long-wavelength transitions are found to be oriented at about 65.0-85.0 degrees with respect to the DNA helix axis. A near perpendicular orientation of the norfloxacin chromophore plane makes it possible to exclude classical groove or surface binding modes. The possibility of a classical intercalation binding mode also can be ruled out from unwinding experiments. However, it is shown that the molecular plane of norfloxacin is near perpendicular relative to the DNA helix axis with a possibility of a bending of the DNA helix at the binding site.
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  • Zaslavsky, Arkady, et al. (författare)
  • Petri nets analysis of transaction and submitter management protocols in mobile distributed computing environment
  • 1995
  • Ingår i: Proceedings. - Los Alamitos, Calif : IEEE Communications Society. - 0818671807 ; , s. 292-299
  • Konferensbidrag (refereegranskat)abstract
    • This paper proposes and discusses a simulation model of a message and transaction queuing protocol which supports submission of transactions from mobile workstations in a distributed multidatabase environment. The model is based on coloured Petri nets formalism. A hierarchical coloured Petri nets model describing the two key components of the protocol, namely message queuing facility and transaction queuing facility, is constructed and analyzed using the software tool Design/CPN. Simulation of message and transaction processing algorithms has been carried out to verify and refine the design of the proposed protocol. The tool has also enabled us to assess the practicality of the developed approach and study the impact of the protocol implementation. Finally, the results of simulation are analysed and discussed
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  • Resultat 1-6 av 6

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