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- Ramström, Olof, et al.
(författare)
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Recent Advances in the Use of Molecularly Imprinted Materials in Separation and Synthesis
- 1998
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Ingår i: Molecular and Ionic Recognition with Imprinted Polymers. - Washington, DC : American Chemical Society. - 0097-6156. - 9780841235748 - 9780841216853 ; 703, s. 82-89
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Bokkapitel (refereegranskat)abstract
- Molecularly imprinted materials, prepared using self-assembly imprinting protocols using only non-covalent interactions, can be used as chromatography media in aqueous phase. The recognition properties are highly dependent on the interacting species used in the imprinting protocol. Ionic interactions, together with strong hydrogen bonding, represent useful means of obtaining recognition. With increasing levels of water in the recognition media, the hydrophobic effect comes into play. Molecularly imprinted materials can furthermore be used as auxiliary agents in enzymatic syntheses in water-saturated organic phases. When materials molecularly imprinted for the reaction product were applied to the thermolysin-catalyzed aspartame synthesis, an increase in yield was observed. Introduction of a thermodynamic trap provided by the molecularly imprinted matrices allowed a non-favorable equilibrium for the thermolysin reaction to be pushed in the forward direction.
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| 2. |
- Yu, Cong
(författare)
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Molecular Recognition Studies Based on Imprinting Technology
- 1998
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- In this thesis, I have focused on five main research areas: 1). A new type of imprinted polymer has been developed, namely the amide MIP. The amide functional monomers showed better hydrogen bonding capabilities in polar organic solvents and the resulting polymers exhibited good enantiomeric recognition properties in organic media. Furthermore, by using the amide monomers instead of the carboxyl monomers, imprinted polymers could be prepared without the excess of charged groups which often leads to the problem of swelling and non-specific interactions. 2). By using the amide MIPs, the recognition mechanism of imprinted polymers has been studied in detail. It was found that the shape, size and the nature and distribution of the functional groups of the sample molecule all had a marked influence on the selectivity. 3). The solvent effect was also studied, it was found that the recognition properties of imprinted polymers could be dramatically influenced by the assay solvent system used. By using a simply optimized solvent system, our results showed that the imprinted polymers? recognition performances could be greatly improved compared with the results obtained previously. 4). The amide MIPs were also found to be capable of selective recognition in aqueous media. Our results showed that the selective binding in organic media by imprinted polymers was mainly determined by the polar interactions between the sample molecules and the recognition sites, whereas in aqueous media, hydrophobic interactions played a major role. 5). Attempts have also been made to use imprinted polymers to separate chiral compounds, as enzyme and receptor mimics and to make imprinted polymer beads in aqueous media.
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